Protective effect of N2-mercaptopropionylglycine on rats and dogs liver during ischemia/reperfusion process

Abstract

These results suggest a significant release of free radicals of oxygen in the process and that N2-mercaptopropionylglycine may have a significant protective effect on liver parenchyma when submitted to ischemia/reperfusion.

The Current State of Knowledge of Hepatic Ischemia-Reperfusion Injury Based on Its Study in Experimental Models

“…Small animals such as mice and rats are exceptionally useful because they are easy to manage, present minimal logistical, financial, or ethical problems, and provide the potential for genetic alterations (e.g., transgenic and knock-out animals). However, an important drawback is that the results of studies performed in small animals are of limited applicability to human beings due to their varying size and anatomy of the liver and their faster metabolism [15]. Large animals such as pigs, sheep, and dogs exhibit greater similarity in their anatomy and physiology to human beings.…”

“…However, their use is restricted by serious logistical and financial difficulties and often by ethical concerns. Furthermore, the technical possibilities of blood and tissue processing are extremely restricted because of the limited availability of immunological tools for use in large animal species [15]. …”

Experimental Models in Liver Surgery

“…Small animals such as mice and rats are exceptionally useful because they are easy to manage, present minimal logistical, financial, or ethical problems, and provide the potential for genetic alterations (e.g., transgenic and knockout animals). However, an important drawback is that the results of studies performed in small animals are of limited applicability to human beings due to their varying size and anatomy of the liver and their faster metabolism [17]. Large animals such as pigs, sheep, and dogs exhibit greater similarity in their anatomy and physiology to human beings.…”

Efeito da solução salina hipertônica nas lesões resultantes da isquemia/reperfusão hepática: estudo experimental em ratos

“…Durante a fase tardia da reperfusão, há maior ativação das células de Kupffer e, principalmente, ativação dos neutrófilos recrutados na microcirculação durante a fase inicial. 27,28,29 O fígado é o órgão que contém o maior número de macrófagos (células de Kupffer) que são as principais fontes de EOR durante a fase inicial da reperfusão. A produção de EOR pelas CK ativadas é potencializada pela ativação de fatores do complemento, prolongando o estresse oxidativo.…”

“…Destacam-se o pré-condicionamento hepático, a oclusão vascular intermitente e a utilização de algumas substâncias como antioxidantes, antagonistas dos fatores ativadores de plaquetas e anti-TNF. 18,29,47,48,49 A administração de solução salina hipertônica (SSH) a 7,5% representa mais uma das possíveis terapias que podem ser empregadas no tratamento da I/R. 10,50,51 Vários trabalhos demonstraram a eficácia da administração endovenosa de solução salina hipertônica no tratamento do choque hemorrágico, que é um modelo de isquemia/reperfusão que envolve vários órgãos.…”