Protective Effect of Melatonin on Water Avoidance Stress Induced Degeneration of the Bladder

Çetınel, Şule; Ercan, Feríha; Çikler, Esra; Contuk, Gazi; Şener, Göksel

doi:10.1097/01.ju.0000145891.35810.56

Cited by 35 publications

(40 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus future studies should be pursued in anesthetized rats where potential effects of the outlet including the urethra and EUS can be evaluated after RVS. RVS-induced increases in voiding frequency are similar to other reports regarding several other animal models of stress including social defeat (4,15,46,74), nontraumatic immobilization/restraint (11,74), and WAS (14,54,62). We suggest that the 7-day RVS model has several advantages compared with other stress models.…”

Section: Rvs Induces Changes In Urinary Bladder Functionsupporting

confidence: 73%

“…The most well-established animal models of stress used to examine effects on urinary bladder structure and function include the resident-intruder model (15,22,74), immobilization stress (10,11), water avoidance stress (WAS) (14,54,55,62), and electrical footshock (7,55). Although these stress paradigms produce bladder dysfunction and target tissue (i.e., urinary bladder) abnormalities similar to conditions like IC/BPS, the data in some cases are conflicting, and the stress models used in these studies may not be relevant to the variety of life stressors experienced by humans on a daily basis.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Repeated variate stress in male rats induces increased voiding frequency, somatic sensitivity, and urinary bladder nerve growth factor expression

Merrill

Malley

Vizzard

2013

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

Merrill L, Malley S, Vizzard MA. Repeated variate stress in male rats induces increased voiding frequency, somatic sensitivity, and urinary bladder nerve growth factor expression. Am J Physiol Regul Integr Comp Physiol 305: R147-R156, 2013. First published May 8, 2013 doi:10.1152/ajpregu.00089.2013.-Stress exacerbates symptoms of functional lower urinary tract disorders including interstitial cystitis (IC)/bladder pain syndrome (BPS) and overactive bladder (OAB) in humans, but mechanisms contributing to symptom worsening are unknown. These studies address stress-induced changes in the structure and function of the micturition reflex using an animal model of stress in male rats. Rats were exposed to 7 days of repeated variate stress (RVS). Target organ (urinary bladder, thymus, adrenal gland) tissues were collected and weighed following RVS. Evans blue (EB) concentration and histamine, myeloperoxidase (MPO), nerve growth factor (NGF), brain-derived neurotropic factor (BDNF), and CXCL12 protein content (ELISA) were measured in the urinary bladder, and somatic sensitivity of the hindpaw and pelvic regions was determined following RVS. Bladder function was evaluated using continuous, open outlet intravesical infusion of saline in conscious rats. Increases in body weight gain were significantly (P Յ 0.01) attenuated by day 5 of RVS, and adrenal weight was significantly (P Յ 0.05) increased. Histamine, MPO, NGF, and CXCL12 protein expression was significantly (P Յ 0.01) increased in the urinary bladder after RVS. Somatic sensitivity of the hindpaw and pelvic regions was significantly (P Յ 0.01) increased at all monofilament forces tested (0.1-4 g) after RVS. Intercontraction interval, infused volume, and void volume were significantly (P Յ 0.01) decreased after RVS. These studies demonstrate increased voiding frequency, histamine, MPO, NGF, and CXCL12 bladder content and somatic sensitivity after RVS suggesting an inflammatory component to stress-induced changes in bladder function and somatic sensitivity. micturition; stress; nerve growth factor; ELISA; somatic sensitivity STRESS CONTRIBUTES to symptom exacerbation in many disease states, including functional disorders of the urinary bladder such as overactive bladder (OAB) and interstitial cystitis (IC)/ bladder pain syndrome (BPS) (3,38,58,72). Urinary frequency is a common symptom among patients with OAB or IC/BPS, although the end result of frequent voiding may differ [reduce incontinence episodes (OAB) vs. reduce pain with bladder filling (IC/BPS)]. Patients with IC/BPS report symptom worsening during stress, as do patients with other disorders associated with IC/BPS including rheumatoid arthritis, psoriasis, and irritable bowel syndrome (47,72). Symptom worsening during stress may be due, in part, to disruption of the hypothalamic-pituitary-adrenal (HPA) axis. Cortisol, through feedback on the HPA axis, normally acts to attenuate inflammation (47); however, abnormalities in the feedback may cause dysregulation of the inflammatory response. Therefore, patients...

show abstract

Section: Rvs Induces Changes In Urinary Bladder Functionsupporting

confidence: 73%

mentioning

confidence: 99%

Repeated variate stress in male rats induces increased voiding frequency, somatic sensitivity, and urinary bladder nerve growth factor expression

Merrill

Malley

Vizzard

2013

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…Literatür bilgisine paralel olarak çalışmamızda SKS uygulanan erkek sıçanların mesanelerinde dişi sıçanlara benzer morfolojik ve biyokimyasal değişiklikler gözlenmiştir (17,21,22). OT tedavisi sonucunda, SKS'ye bağlı olarak mesane mukozasında polimorf nüveli lökosit ve degranüle olmuş mast hücre sayısında artış tespit edilmiştir.…”

Section: Discussionunclassified

“…Stresin ağrılı bir mesane hastalığı olan İS'in patofizyolojisinde önemli bir rol oynadığı birçok klinik ve deneysel çalışmada gösterilmiştir (3,4). SKS uygulanmış dişi sıçan mesane dokusuna ait ışık mikroskobik incelemelerde, lamina propriyada polimorf nüveli lökosit infiltrasyonu, ürotelyum hücrelerinde hasar, GAG tabakasında düzensizlik ve granüllü ve degranüle olmuş mast hücre artışı izlenmiştir (17,21,22). Vakuol oluşumu, perinüklear sisterna dilatasyonu, pleomorfik ve uniform mikrovillus oluşumu ve intersellüler sıkı bağlantılarda belirgin bir dilatasyon elektron mikroskobik seviyesindeki incelemeler ile gösterilmiştir (17).…”

Section: Discussionunclassified

“…Sistematik rastgele örnekleme yapılarak toplam olarak 5 kesit (ilk kesit rastgele olmak üzere her 5 kesitten biri) alındı. Bilgisayar görüntüleme yardımıyla her bir kesitte x100 büyütmede rastgele seçilen 10 ayrı alanda (her bir alan 0.0908 mm 2 ) x400 büyütmede granüllü ve degranüle olmuş mast hücre sayımı yapıldı ve mast hücre yoğunluğu her bir alandaki hücre sayısı olarak ifade edildi (17).…”

Section: Mast Hücre Sayımıunclassified

See 1 more Smart Citation

Sudan kaçınma stresi uygulanmış erkek sıçan mesanesinde oksitosin etkisi: Işık ve elektron mikroskobik inceleme

Ak¹,

Dülger²,

Şehirli³

et al. 2015

mpj

View full text Add to dashboard Cite

Water avoidance stress results in an altered voiding phenotype in male mice

McGonagle

Smith

Butler

et al. 2012

Neurourology and Urodynamics

View full text Add to dashboard Cite

show abstract

Protective Effect of Melatonin on Water Avoidance Stress Induced Degeneration of the Bladder

Abstract: The results show that melatonin reverses WAS induced degenerative changes in the bladder.

Cited by 35 publications

References 17 publications

Repeated variate stress in male rats induces increased voiding frequency, somatic sensitivity, and urinary bladder nerve growth factor expression

Repeated variate stress in male rats induces increased voiding frequency, somatic sensitivity, and urinary bladder nerve growth factor expression

Sudan kaçınma stresi uygulanmış erkek sıçan mesanesinde oksitosin etkisi: Işık ve elektron mikroskobik inceleme

Water avoidance stress results in an altered voiding phenotype in male mice

Contact Info

Product

Resources

About