PROTECTIVE EFFECT OF LYSOSTAPHIN FROM STAPHYLOCOCCUS SIMULANS AGAINST GROWTH OF STAPHYLOCOCCUS AUREUS IN MILK AND SOME OTHER FOOD PRODUCTS

Abstract

The effect of lysostaphin from Staphylococcus simulans expressed in Escherichia coli TOP10 strain on Staphylococcus aureus used for inoculation of milk, ground pork and mayonnaise salad was investigated. The populations of this pathogen in ultrahigh-temperature milk preserved at 4C by lysostaphin added up to concentrations of 1.5 or 3.0 mg/mL were reduced by 0.73 and 0.92 log(cfu/mL) in control samples without enzyme addition. The protective influence of lysostaphin was diminished in case of milk storage (20C) prolonged up to 24 h. Furthermore, a final reduction level by 0.92 log(cfu/mL) was achieved after 24 h of pork storage. The smaller and more dependent on enzyme concentration inactivation of S. aureus was observed in the case of the mayonnaise salad, and it led to the conclusion that some food components or proteolytic enzymes originating from other bacteria caused lysostaphin inactivation.

Peptidoglycan hydrolases-potential weapons against Staphylococcus aureus

“…Also, purified recombinant lysostaphin expressed in E. coli cells has been developed for food protection against S . aureus by Szweda et al ( 2007 ). The population of S .…”

Food applications of bacterial cell wall hydrolases

“…Lysostaphin was shown already a long time ago to suppress and even slightly kill S. aureus cells in various foods, a finding that has been recently confirmed in milk, mayonnaise salad and ground pork [33]. Oyster lysozyme in calcium alginate coatings effectively suppressed growth of Listeria monocytogenes and Salmonella anatum in smoked salmon samples, but its effectiveness was not different from that of HEWL [34].…”

Chitosan-protein scaffolds loaded with lysostaphin as potential antistaphylococcal wound dressing materials

“…The recombinant lysostaphin was produced in the cells of Escherichia coli TOP10F' strain (Invitrogen, Carlsbad, CA), transformed with the plasmid pBAD2Lys and constructed earlier in our laboratory (Szweda et al . ). The production of the enzyme was carried out in a 5‐l bioreactor (Biostat C; Braun Co., Germany) according to the procedure optimized by Szweda et al .…”