Protective effect of linoleic acid against inflammatory reactions by mast cell via caspase‐1 cascade pathways

Abstract

Blockade of caspase‐1 was reported to be a new target for allergic inflammation treatment. Here, we present the effect of linoleic acid (LA), a constituent of Allium hookeri (AH), to alleviate mast cell‐mediated allergic inflammation. Pretreatment of LA and AH significantly reduced caspase‐1 activation without displaying host cell cytotoxicity in activated human mast cells. IC50 value of LA on caspase‐1 activity is 0.014 μM. LA and AH pretreatment effectively regulated increased levels of interleukin (IL)‐1β, IL‐6, IL‐8, thymic stromal lymphopoietin, and tumor necrosis factor on activated human mast cells. Moreover, LA and AH were effective against activations of nuclear factor‐κB and mitogen‐activated protein kinases in human mast cells. In summary, LA and AH alleviate allergic inflammatory reactions via blocking caspase‐1 cascade signaling pathway. These results provide evidence for the anti‐allergic inflammatory properties of LA and AH and corroborate its potential use for the treatment and prevention of allergic diseases. Practical applications Allium hookeri (AH) is used as traditional food to treat various diseases and contains an essential fatty acid, linoleic acid (LA). LA and AH alleviate mast cell‐mediated allergic inflammatory reactions via inhibiting inflammatory mediators. These results provide evidence for the anti‐allergic inflammatory properties of LA and AH and corroborate its potential use for the treatment and prevention of allergic diseases.

Characterization of plasma metabolites and proteins in patients with herpetic neuralgia and development of machine learning predictive models based on metabolomic profiling

“…Regarding metabolomics in PHN patients, the dysregulated metabolites were mostly related to four metabolic pathways: fatty acid, tryptophan, glutamate, and ornithine. Dysregulated fatty acid metabolites were identified as vital inflammatory mediators of arachidonic acid, and its metabolites could activate the endogenous transmitter TRPV1 to increase the sensitivity of nerves ( Zimmer et al, 2018 ; Kim et al, 2019 ; Osthues and Sisignano, 2019 ). More importantly, the activation of the tryptophan metabolic pathway by inflammatory stimuli in the PHN patients led to abnormalities in 5-HT metabolism and reuptake and might respond to the absence of the action of the downstream inhibitory system and increased neuro sensitivity leading to the development of PHN ( Roth et al, 2021 ).…”

Chemical Fingerprint Analysis for Discovering Markers and Identifying Saussurea involucrata by HPLC Coupled with OPLS-DA

“…SIP–B2-85-3 (120 mg), which was separated by HPLC, followed by eluting with MeCN–H 2 O (90%), yielding linolenic acid (7.6 mg) and linoleic acid (8.2 mg). Significantly, the two polyunsaturated fatty acids have anti-inflammatory activity [ 32 , 33 ], showing potential for medicinal applications.…”

Mechanism and Experimental Verification of Rhodiola rosea L. for the Treatment of Acute Lung Injury Through Network Pharmacology and Molecular Docking

“…Linoleic acid demonstrates efficacy in diminishing, IL-6, Interleukin 8, and IL-1β content in allergic inflammation. 26 Kae, a natural flavonoid, displays remarkable antiinflammatory activity. It reduces Interleukin 4 and Interleukin 13 expression, inhibiting inflammation in specific dermatitis mouse models.…”