Protective effect of Lactobacillus fermentum CQPC04 on dextran sulfate sodium–induced colitis in mice is associated with modulation of the nuclear factor-κB signaling pathway

Zhou, Xianrong; Liu, Huazhi; Zhang, Jing; Mu, Jianfei; Zalán, Zsolt; Hegyi, Ferenc; Takács, Krisztina; Zhao, Xin; Du, Muying

doi:10.3168/jds.2019-16840

Cited by 43 publications

(32 citation statements)

References 51 publications

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“…Park et al showed that, in a dextran sulfate sodium-induced colitis, mice fed with L. acidophilus showed an increase in Treg cells and splenic IL-10 coupled with a reduction of splenic IL-17 and colonic IL-6, TNF-b, IL-1b, and IL-17 (97). In a recent study, the administration of L. fermentum CQPC04 significantly inhibited pro-inflammatory cytokines production (IFN-g, IL-1b, TNF-a, IL-6, and IL-12), and promoted the release of IL-10 in serum ameliorating the colonic damage (98). Choi et al demonstrated that the oral administration of L. plantarum strain CAU1055 significantly decreased the levels of inducible NOS, COX-2, TNF-a, and IL-6 (99).…”

Section: Animal Studiesmentioning

confidence: 95%

Anti-Inflammatory and Immunomodulatory Effects of Probiotics in Gut Inflammation: A Door to the Body

et al. 2021

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Hosting millions of microorganisms, the digestive tract is the primary and most important part of bacterial colonization. On one side, in cases of opportunistic invasion, the abundant bacterial population inside intestinal tissues may face potential health problems such as inflammation and infections. Therefore, the immune system has evolved to sustain the host–microbiota symbiotic relationship. On the other hand, to maintain host immune homeostasis, the intestinal microflora often exerts an immunoregulatory function that cannot be ignored. A field of great interest is the association of either microbiota or probiotics with the immune system concerning clinical uses. This microbial community regulates some of the host’s metabolic and physiological functions and drives early-life immune system maturation, contributing to their homeostasis throughout life. Changes in gut microbiota can occur through modification in function, composition (dysbiosis), or microbiota–host interplays. Studies on animals and humans show that probiotics can have a pivotal effect on the modulation of immune and inflammatory mechanisms; however, the precise mechanisms have not yet been well defined. Diet, age, BMI (body mass index), medications, and stress may confound the benefits of probiotic intake. In addition to host gut functions (permeability and physiology), all these agents have profound implications for the gut microbiome composition. The use of probiotics could improve the gut microbial population, increase mucus-secretion, and prevent the destruction of tight junction proteins by decreasing the number of lipopolysaccharides (LPSs). When LPS binds endothelial cells to toll-like receptors (TLR 2, 4), dendritic cells and macrophage cells are activated, and inflammatory markers are increased. Furthermore, a decrease in gut dysbiosis and intestinal leakage after probiotic therapy may minimize the development of inflammatory biomarkers and blunt unnecessary activation of the immune system. In turn, probiotics improve the differentiation of T-cells against Th2 and development of Th2 cytokines such as IL-4 and IL-10. The present narrative review explores the interactions between gut microflora/probiotics and the immune system starting from the general perspective of a biological plausibility to get to the in vitro and in vivo demonstrations of a probiotic-based approach up to the possible uses for novel therapeutic strategies.

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Section: Animal Studiesmentioning

confidence: 95%

Anti-Inflammatory and Immunomodulatory Effects of Probiotics in Gut Inflammation: A Door to the Body

et al. 2021

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“…After rinsing in 1 × TBST five times, the secondary antibodies (Thermo Fisher Scientific, Inc.) were incubated at 25°C for 1 hr. Last, SuperSignal West Pico PLUS Chemiluminescent Substrate was spread on the PVDF membrane and visualized on the iBright Fl1000 system (Thermo Fisher Scientific, Inc.) (Zhou et al, 2019).…”

Section: Methodsmentioning

confidence: 99%

Protective effect of Lactobacillus plantarum YS3 on dextran sulfate sodium‐induced colitis in C57BL/6J mice

Fan

Long

et al. 2021

J. Food Biochem.

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The protective effect of Lactobacillus plantarum YS3 (LP-YS3) on ulcerative colitis (UC) was assessed using a mouse model of dextran sodium sulfate (DSS)-induced colitis. Different concentrations of LP-YS4 were administered to the experimental mice by daily gavage. Several inflammatory and biochemical indices, such as interleukin-2 (IL-2), interleukin-10 (IL-10), interleukin-6 (IL-6), interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNFα), glutathione (GSH), malondialdehyde (MDA), myeloperoxidase (MPO), and nitric oxide (NO), were examined in mouse serum and colon tissue. The mRNA and protein expression levels of c-Kit, CXC chemokine receptor type 2 (CXCR2), interleukin-8 (IL-8), and stem cell factor (SCF) in mouse colon tissue were assessed using Western blot and quantitative polymerase chain reaction (qPCR) assays. The findings indicated that LP-YS3 remarkably decreased the disease activity index (DAI) of UC mice (p < .05), inhibited colon length shortening induced by UC, and elevated the value of colon weight/length ratio. LP-YS3 could also markedly reduce (p < .05) the activities of MDA, MPO, and NO; while an increase in the GSH content in the colonic tissue of UC mice. Moreover, LP-YS3 remarkably increased (p < .05) the serum level of IL-2 in UC mice, while reduced those of IL-10, IL-6, IL-1β, TNFα cytokines. qPCR data revealed that LP-YS3 could markedly upregulate the expression levels of c-Kit and SCF, while downregulate those of CXCR2 and IL-8 in the colonic tissue of UC mice (p < .05). LP-YS3 exerted an outstanding protective effect on DSS-induced colitis in C57BL/6J mice, especially at higher concentrations. Practical applications Lactobacillus plantarum YS3 is a newly isolated and identified lactic acid bacteria. This study confirmed that L. plantarum YS3 can inhibit colitis and has good probiotic potential, which needs further development and utilization.

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“…In a previous study, supplementation with Crytocarpa procera bark extract attenuated the colonic mucosal damage and decreased the serum IFN-γ and IL-1β levels in DSS-induced colitis mice [39]. Supplementation with L. fermentum CQPC04 ameliorated the myeloperoxidase activity and pro-inflammatory cytokines e.g., TNF-α, IFN-γ, IL-1β, IL-6 and IL-12 in the experimental colitis model [40]. These findings suggest that the administration of FP alleviated inflammation by down-regulating the level of serum pro-inflammatory cytokines in DSS-induced colitis mice.…”

Section: Effects Of Fp On the Serum Inflammatory Cytokinesmentioning

confidence: 93%

Protective Effect of Prunus mume Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis

Kim

Won

Cho

et al. 2020

Foods

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The fruit of Prunus mume (PM) is widely cultivated in East Asia, and it has been used as a folk medication for gastrointestinal disorders, e.g., diarrhea, stomach ache and ulceration. In this study, the pectinase-treated PM juice (PJ) was fermented with Lactobacillus strains containing fundamental organic acids and free amino acids. The PJ fermented with Lactobacillus plantarum and L. casei (FP) was investigated for its protective effect in dextran sodium sulfate (DSS)-induced colitis mice model. The administration of FP reduced lipid peroxidation and histopathological colitis symptoms, e.g., shortening of the colon length, depletion of mucin, epithelial injury and ulceration, in colonic tissues. The FP-supplemented group showed the alleviation of pro-inflammatory cytokines. Compared with the DSS control group, the supplementation of FP significantly reduced the levels of serum interferon-γ (IFN-γ), interleukin (IL)-1β, IL-6, IL-12 and IL-17 as well as colonic tumor necrosis factor-α, IFN-γ, IL-12 and IL-17. Furthermore, the DSS-induced TUNEL-positive area was significantly reduced by the FP supplementation. These results show that the supplementation of FP fermented with mixed lactic acid bacteria, L. plantarum and L. casei, elucidated the protective effect in DSS-induced colitis mice. Hence, this study suggests that FP can be utilized as a natural therapeutic agent for colitis and intestinal inflammation.

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Protective effect of Lactobacillus fermentum CQPC04 on dextran sulfate sodium–induced colitis in mice is associated with modulation of the nuclear factor-κB signaling pathway

Cited by 43 publications

References 51 publications

Anti-Inflammatory and Immunomodulatory Effects of Probiotics in Gut Inflammation: A Door to the Body

Anti-Inflammatory and Immunomodulatory Effects of Probiotics in Gut Inflammation: A Door to the Body

Protective effect of Lactobacillus plantarum YS3 on dextran sulfate sodium‐induced colitis in C57BL/6J mice

Protective Effect of Prunus mume Fermented with Mixed Lactic Acid Bacteria in Dextran Sodium Sulfate-Induced Colitis

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