Protective effect of <i>Scutellaria</i> species on AAPH-induced oxidative damage in human erythrocyte

Salini, Sasidharan; Divya, Menon Kunnathully; Chubicka, Thomas; Meera, Nair; Fulzele, Devanand P.; Ragavamenon, Achuthan C.; Babu, T. Ramesh

doi:10.1515/jbcpp-2015-0081

Cited by 5 publications

(3 citation statements)

References 28 publications

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“…In order to monitor the antioxidant characteristics of N‐IgG and AA‐IgG, protection from AAPH induced RBC hemolysis was done . Blood sample from healthy person was collected in Ethylenediaminetetraacetic acid (EDTA) vial and centrifuged at 2500 rpm for 10 min at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…In order to monitor the antioxidant characteristics of N-IgG and AA-IgG, protection from AAPH induced RBC hemolysis was done. [18][19][20] Blood sample from healthy person was collected in Ethylenediaminetetraacetic acid (EDTA) vial and centrifuged at 2500 rpm for 10 min at 4 C. Plasma and buffy coat were removed and the packed RBC pellet were washed three times with PBS (0.9% NaCl in 10 mM sodium phosphate buffer, pH 7.4) and then suspended in PBS to obtain 10% (v/v) cell suspension. Hundred microliters of cells were added in each well of flat bottom 96-well plate followed by addition of 10 μM of N-IgG and AA-IgG samples.…”

Section: Free Radical-induced Rbc Hemolysismentioning

confidence: 99%

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Molecular docking explores heightened immunogenicity and structural dynamics of acetaldehyde human immunoglobulin G adduct

et al. 2019

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Acetaldehyde is a metabolite of ethanol, an important constituent of tobacco pyrolysis and the aldehydic product of lipid peroxidation. Acetaldehyde induced toxicity is mainly due to its binding to cellular macromolecules resulting in the formation of stable adducts accompanied by oxidative stress. The aim of this study was to characterize structural and immunological alterations in human immunoglobulin G (IgG) modified with acetaldehyde in the presence of sodium borohydride, a reducing agent. The IgG modifications were studied by various physicochemical techniques such as fluorescence and CD spectroscopy, free amino group estimation, 2,2‐azobis 2‐amidinopropane (AAPH) induced red blood cell hemolysis as well as transmission electron microscopy. Molecular docking was also employed to predict the preferential binding of acetaldehyde to IgG. The immunogenicity of native and acetaldehyde‐modified IgG was investigated by immunizing female New Zealand white rabbits using native and modified IgG as antigens. Binding specificity and cross reactivity of rabbit antibodies was screened by competitive inhibition ELISA and band shift assays. The modification of human IgG with acetaldehyde results in quenching of the fluorescence of tyrosine residues, decrease in free amino group content, a change in the antioxidant property as well as formation of cross‐linked structures in human IgG. Molecular docking reveals strong binding of IgG to acetaldehyde. Moreover, acetaldehyde modified IgG induced high titer antibodies (>1:12800) in the experimental animals. The antibodies exhibited high specificity in competitive binding assay toward acetaldehyde modified human IgG. The results indicate that acetaldehyde induces alterations in secondary and tertiary structure of IgG molecule that leads to formation of neo‐epitopes on IgG that enhances its immunogenicity.

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Section: Methodsmentioning

confidence: 99%

Section: Free Radical-induced Rbc Hemolysismentioning

confidence: 99%

Molecular docking explores heightened immunogenicity and structural dynamics of acetaldehyde human immunoglobulin G adduct

et al. 2019

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“…The anti-hemolytic activity induced by a peroxyl radical initiator, AAPH was measured according to the method established by Salini, Divya, Chubicka, Meera, Fulzele, Ragavamenon, Babu 24 . Two hundred microliters of blood were mixed with 10 μL of plant extract at various concentrations (0-100 μg/mL) and then 600 μL of AAPH (10%) was added.…”

Section: Anti-hemolytic Potentialmentioning

confidence: 99%

Antioxidant, Anti-Inflammatory and Anticancer Activities of the Methanolic Extract of Thottea siliquosa: An In Vitro and In Silico Study

Koottasseri¹,

Babu²,

Augustin³

et al. 2021

PRA

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Background: Oxidative stress and inflammation are the predominant cause of chronic diseases including multiple forms of cancers. Due to the prominent roles, prevention of these oxidative stress and inflammation is considered to be a target for preventing these disorders. Various natural products and plant extracts prevent the process of free radical-induced damages. Objectives: The present study evaluated the biological properties of Thottea siliquosa, belonging to the family Aristolochiaceae, which is a traditionally used Ayurvedic plant. Methods: Antioxidant assays carried out were DPPH, FRAP, hydrogen peroxide scavenging, and hemolysis inhibition assay; nitric oxide and lipoxygenase inhibition assays were used for anti-inflammatory studies. Anticancer activity was done using human endometrial and breast cancer cells by MTT assay. Bioactive compounds present in T. siliquosa were identified by LCMS and each was docked with various cancer targets including EGFR, VEGFR, GST, COX2, and Lipooxygenase. Results: The results of the present study showed antioxidant properties for the methanolic crude extract of T. siliquosa as DPPH radical scavenging (110.40 ± 4.5µg/mL), FRAP capacity (41.1 ± 6.2), peroxide scavenging (233.4 ± 14.2µg/mL). Besides, anti-inflammatory properties are also evident in terms of nitric oxide radical scavenging (28.76± 3.9 µg/mL) and lipoxygenase inhibition (39.2 ± 3.2µg/mL) assays. In silico analysis confirmed the inhibitory potential of the bioactive compounds of T. siliquosa against cancer drug targets such as EGFR, VEGFR, and inflammatory enzymes cyclooxygenase as well as lipooxygenase. Further, the anticancer activity of the extract has been identified against human endometrial and breast cancer cells. The possible mechanism of anticancer action of the extract is mediated through the apoptosis induction mediated through increased caspase and APAF-1 expression. Conclusion: The study thus concludes that T. siliquosa showed significant antioxidant, anti-inflammatory and anticancer properties. Further studies together with a bioassay-guided fractionation may identify possible bioactive compounds.

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Baicalein attenuates oxidative damage in mice haematopoietic cells through regulation of PDGFRβ

Ren,

Feng,

Hong

et al. 2024

Molecular and Cellular Probes

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Protective effect of Scutellaria species on AAPH-induced oxidative damage in human erythrocyte

Abstract: Results indicate that S. colebrookiana and S. violacea are capable of protecting erythrocytes from oxidative damage. This cytoprotective effect of the extract is possibly by its antioxidant property.

Cited by 5 publications

References 28 publications

Molecular docking explores heightened immunogenicity and structural dynamics of acetaldehyde human immunoglobulin G adduct

Molecular docking explores heightened immunogenicity and structural dynamics of acetaldehyde human immunoglobulin G adduct

Antioxidant, Anti-Inflammatory and Anticancer Activities of the Methanolic Extract of Thottea siliquosa: An In Vitro and In Silico Study

Baicalein attenuates oxidative damage in mice haematopoietic cells through regulation of PDGFRβ

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