Protective Effect of <i>Lycium barbarum</i> Polysaccharides Against Doxorubicin‐induced Testicular Toxicity in Rats

Xin, Yanfei; You, Zhang; Gao, Haiyan; Zhou, Guoliang; Chen, Yunxiang; Yu, Jian; Xuan, Yue

doi:10.1002/ptr.3633

Cited by 62 publications

(42 citation statements)

References 39 publications

(72 reference statements)

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“…LBPs were delivered into miR‐1 Tg mice at 3 months of age through intragastric administration at a dosage of 200 mg/kg/d22, 26 for 1 month. The age‐matched WT mice and miR‐1 Tg mice were received 0.9% NaCl (0.2 mL/d) as the vehicle.…”

Section: Methodsmentioning

confidence: 99%

Lycium barbarum polysaccharides restore adverse structural remodelling and cardiac contractile dysfunction induced by overexpression of microRNA‐1

Zhang

Niu

et al. 2018

J Cellular Molecular Medi

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MicroRNA‐1 (miR‐1) stands out as the most prominent microRNA (miRNA) in regulating cardiac function and has been perceived as a new potential therapeutic target. Lycium barbarum polysaccharides (LBPs) are major active constituents of the traditional Chinese medicine based on L. barbarum. The purpose of this study was to exploit the cardioprotective effect and molecular mechanism of LBPs underlying heart failure. We found that LBPs significantly reduced the expression of myocardial miR‐1. LBPs improved the abnormal ECG and indexes of cardiac functions in P‐V loop detection in transgenic (Tg) mice with miR‐1 overexpression. LBPs recovered morphological changes in sarcomeric assembly, intercalated disc and gap junction. LBPs reversed the reductions of CaM and cMLCK, the proteins targeted by miR‐1. Similar trends were also obtained in their downstream effectors including the phosphorylation of MLC2v and both total level and phosphorylation of CaMKII and cMyBP‐C. Collectively, LBPs restored adverse structural remodelling and improved cardiac contractile dysfunction induced by overexpression of miR‐1. One of the plausible mechanisms was that LBPs down‐regulated miR‐1 expression and consequently reversed miR‐1‐induced repression of target proteins relevant to myocardial contractibility. LBPs could serve as a new, at least a very useful adjunctive, candidate for prevention and therapy of heart failure.

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Section: Methodsmentioning

confidence: 99%

Lycium barbarum polysaccharides restore adverse structural remodelling and cardiac contractile dysfunction induced by overexpression of microRNA‐1

Zhang

Niu

et al. 2018

J Cellular Molecular Medi

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“…Although the adverse effects of DXR are not fully understood, it is known to increase oxidative stress, which suggests that reactive oxygen species (ROS) likely play a role [4]. Oxidants from the environment (e.g.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Rosmarinic Acid on Doxorubicin-Induced Testicular Damage

Üyetürk

Üyetürk²,

Fırat³

et al. 2014

Chemotherapy

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Background: We investigated the protective effects of rosmarinic acid (RA) on testicular damage induced by doxorubicin (DXR) in rats. Methods: In total, 21 rats were divided into 3 groups: the control group that received no treatment, the DXR group that received intraperitoneal (i.p.) DXR on day 7 and the DXR + RA group that received intragastric RA for 10 days with i.p. DXR on day 7. The rats were sacrificed on day 11 for histological and biochemical analyses. To assess oxidative damage, glutathione peroxidase (GPx) and malondialdehyde (MDA) levels were measured. Results: The median modified Johnsen score of the DXR + RA group was higher than that of the DXR group (p = 0.002). The rats with the narrowest seminiferous tubules were in the DXR group (0.17 ± 0.03), and the difference between the DXR + RA and DXR groups was statistically significant (p = 0.002). The number of apoptotic cells in the DXR group was significantly higher than that in the control group, and there were significantly fewer apoptotic cells in the DXR + RA group than in the DXR group (p = 0.002). The MDA level was lowest in the control group and highest in the DXR group, and the level observed in the DXR + RA group significantly lower than that in the DXR group (p = 0.002). The GPx level was highest in the control group, with the level observed in the DXR + RA group significantly higher than that in the DXR group (p = 0.002). The testosterone level was lowest in the DXR group and highest in the control group, and that observed in the DXR + RA group was significantly higher than that in the DXR group (p = 0.018). Conclusions: RA can correct DXR-induced testicular damage through its antioxidant properties. However, the mechanism underlying the effects of RA requires further investigation, and long-term and comparative human studies are also needed.

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“…Oxidative stress that is associated with denaturation and fragmentation has a great role in abnormal sperm production. [21,26] Some previous studies have suggested that doxorubicin also induces DNA fragmentation and chromosomal damage [21,27,28] , leading to increased oxidative stress and increased number of mobile sperm count and abnormal sperm percentage. [29] In a model of Drosophilia melonogaster, vinyl cyclohexene (VCH) exposure for 5 days had increased reactive oxygen and nitrogen species (ROS and RNS), mRNA gene expression of superoxide dismutase, Nrf-2 and MAPK-2, changed antioxidant enzyme levels, and inhibited of δ-ALA -D and AChE activities.…”

Section: Discussionmentioning

confidence: 99%

Protective role of resveratrol on testicular germ cells in mice with testicular toxicity

Özatik

Yiğitaslan

et al. 2017

Turkish Journal of Urology

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Objective: The aim of the present study was to investigate the possible beneficial effects of resveratrol in mice subjected to vinyl cyclohexene dieposide (VCD) -induced testicular toxicity. Material and methods:A total of thirty-six Swiss albino male mice aged 28-days were used in the present study. The study was composed of two stages where mice which received or did not receive VCD (320 mg/kg/day) were administered resveratrol. The animals were assigned into control and resveratrol-treated groups in the first stage and into groups of VCD-and VCD+resveratrol-treated groups in the second stage. At the end of the experiments, relative testicular weight (TW/BW) and dry/wet weight of testis (TDW/ TWW) were calculated. Histological analysis by hematoxylin and eosin (H&E) staining and immunohistochemical staining by BAX and Bcl-2 were performed. Serum testosterone, LH and FSH levels were measured by a commercially available ELISA kit. Results:Resveratrol caused a dose-dependent increase in TW/BW and decrease in TDW/TWW (p<0.05).Resveratrol at a dose of 20 mg/kg resulted in an improvement in testosterone, LH and FSH levels in mice with VCD-induced testicular toxicity (p<0.001). Resveratrol also improved apoptotic index and epithelial cell height of testicular seminipherous tubuli significantly after VCD exposure (p<0.001). Conclusion:Results of the present study suggest that resveratrol can be used as a protective and/or therapeutic agent particularly for cases with male infertility caused by testicular toxicity.

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Protective Effect of Lycium barbarum Polysaccharides Against Doxorubicin‐induced Testicular Toxicity in Rats

Cited by 62 publications

References 39 publications

Lycium barbarum polysaccharides restore adverse structural remodelling and cardiac contractile dysfunction induced by overexpression of microRNA‐1

Lycium barbarum polysaccharides restore adverse structural remodelling and cardiac contractile dysfunction induced by overexpression of microRNA‐1

Protective Effects of Rosmarinic Acid on Doxorubicin-Induced Testicular Damage

Protective role of resveratrol on testicular germ cells in mice with testicular toxicity

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