Protective Effect ofLaminaria japonicawith Probiotics on Murine Colitis

Abstract

Inflammatory bowel disease (IBD) is a chronically relapsing inflammatory disorder of the gastrointestinal tract. Most IBD treatments are unsatisfactory; therefore, various dietary supplements have emerged as promising interventions. Laminaria japonica (LJ) is an edible seaweed used to regulate digestive symptoms. Probiotics have been reported to improve digestive problems and their simultaneous administration with seaweeds has been shown to produce synergistic therapeutic effects. Here, we investigated the effect of LJ combination with probiotics on dextran sodium sulfate-induced colitis model in mice. Aqueous LJ extracts (LJE) at doses from 100 to 300 mg/kg and probiotics at a dose of 300 mg/kg were orally administered for 7 days. Body weight, colon length, histological score, macroscopic damage, and the levels of cytokines IFN-γ, IL-1β, IL-6, IL-10, IL-12 (P40), IL-12 (P70), IL-17, and TNF-α were assessed. LJE alone caused a significant improvement of colitis signs such as colon length, histological score, and IL-1β and IL-6 production. LJE and probiotics demonstrated a synergistic effect by the histological score and levels of IL-1β, IL-6, and IL-12 (P40) but not IFN-γ, IL-10, and IL-12 (P70). In conclusion, LJE was effective in inducing protection against colitis in mice and acted synergistically with probiotics.

Amelioration of the DSS‑induced colitis in mice by pretreatment with 4,4'‑diaponeurosporene‑producing Bacillus subtilis

“…A shorter colon length and bleeding are considered as a hallmark of experimental colitis ( 32 , 33 ). Our result revealed that oral administration of B.s-Dia significantly increased colon length in experimental colitis, while B.s oral administration had no significantly effect on colon length ( Fig.…”

Laminaria japonica Extract Enhances Intestinal Barrier Function by Altering Inflammatory Response and Tight Junction-Related Protein in Lipopolysaccharide-Stimulated Caco-2 Cells

“…Laminaria japonica , a type of brown seaweed, has been studied intensively due to its various health benefits, such as anti-oxidant, antidiabetic, anti-inflammatory, and anti-obesity properties, which potentially prevent and/or treat lifestyle-related diseases [14,15,17,18,39]. In a few countries in Asia, LJ is not only a popular edible seaweed but has also been an alternative medicine to treat gastrointestinal issues since ancient times [40].…”

“…Furthermore, we evaluated functional gut permeability by measuring TEER, resulting in a protective effect by all four LJ extracts on the permeability of the LPS-induced Caco-2 cell monolayer. Ko et al (2014) reported that the administration of LJ water extract (300 mg/kg) improved colitis signs, such as colon length, histological score, and IL-1β, IL-6, and TNF-α production in an in vivo dextran sodium sulfate-induced colitis model [39]. In fact, patients with IBDs appeared to have significantly increased TNF-α and IL-6 levels [49]; therefore, blocking and/or reducing TNF-α and IL-6 signaling has been proposed to be an effective treatment for IBDs.…”

Protective effects of a mixed plant extracts derived fromAstragalus membranaceusandLaminaria japonicaon PTU‐induced hypothyroidism and liver damages

“…The extraction yield of AWE and LWE were 26.91 (w/w %) and 21.15 (w/w %), respectively. The mixing ratio of AWE and LWE powdered aqueous extracts was consisted of 85:15 (AL mix; g/g) based on their optimized concentrations as reported by the previous studies (Ko et al, ; Ren, Zhang, Mu, Sun, & Liu, ; Tseng et al, ; Wang, ) and reconstituted in distilled water for dosing concentrations for further experimental uses.…”