Protective effect of <i>Epicatechin</i> on APAP-induced acute liver injury of mice through anti-inflammation and apoptosis inhibition

Wu, Hao; Xie, Yanni; Xu, Yunfei; Hu, Zehua; Xing, Wei; Huang, Hui; Huang, De-Bin

doi:10.1080/14786419.2018.1503261

Cited by 21 publications

(17 citation statements)

References 11 publications

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“…Moreover, the production of IL-1β, IL-6, NO and phosphorylation of NF-κB (p-p65) in LPS-stimulated RAW264.7 cells were significantly inhibited by six flavonoids. The anti-inflammatory activities of astilbin and (−)-epicatechin were also consistent with several literature reports [27,36,37].…”

Section: Discussionsupporting

confidence: 91%

Antioxidant and Anti-Inflammatory Activities of Six Flavonoids from Smilax glabra Roxb

et al. 2020

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This study aimed to isolate, prepare and identify the main flavonoids from a standardized Smilax glabra flavonoids extract (SGF) using preparative HPLC, MS, 1H NMR and 13C NMR, determine the contents of these flavonoids using UPLC, then compare their pharmacological activities in vitro. We obtained six flavonoids from SGF: astilbin (18.10%), neoastilbin (11.04%), isoastilbin (5.03%), neoisoastilbin (4.09%), engeletin (2.58%) and (−)-epicatechin (1.77%). The antioxidant activity of six flavonoids were evaluated by determining the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical and 2,2′-Azinobis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS+) radical scavenging activity and ferric reducing antioxidant power (FRAP). In addition, the anti-inflammatory activity of six flavonoids were evaluated by determining the production of cytokines (IL-1β, IL-6), nitric oxide (NO) using enzyme linked immunosorbent assay and the NF-κB p65 expression using Western blotting in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results showed that (−)-epicatechin, astilbin, neoastilbin, isoastilbin and neoisoastilbin had strong antioxidant activities, not only in DPPH and ABTS+ radicals scavenging capacities, but in FRAP system. Furthermore, all the six flavonoids could significantly inhibit the secretion of IL-1β, IL-6, NO (p < 0.01) and the protein expression of NF-κB p-p65 (p < 0.01) in LPS-stimulated RAW264.7 cells. This study preliminarily verified the antioxidant and anti-inflammatory activities of six flavonoids in S. glabra.

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Section: Discussionsupporting

confidence: 91%

Antioxidant and Anti-Inflammatory Activities of Six Flavonoids from Smilax glabra Roxb

et al. 2020

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“…EC, a kind of catechin compounds, has been shown to play a significant anti-inflammatory role in a variety of inflammatory diseases, including colitis ( Zhang et al, 2016 ), atherosclerosis ( Morrison et al, 2014 ), acute liver injury ( Wu et al, 2020 ), obesity ( Bettaieb et al, 2016 ), COPD ( Tian et al, 2021 ), etc. It was reported that EGCG, rather than EC, has a significant anti-inflammatory effect in primary human rheumatoid arthritis synovial fibroblasts ( Fechtner et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

(−)-Epicatechin Ameliorates Monosodium Urate-Induced Acute Gouty Arthritis Through Inhibiting NLRP3 Inflammasome and the NF-κB Signaling Pathway

Zhang

et al. 2022

Front. Pharmacol.

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Background: Gouty arthritis is a common and complex inflammatory disease that will reduce the life quality of human beings (−)-Epicatechin (EC) is famous for antioxidant and anti-inflammatory activities. Thus, the aim of this study was to investigate the therapeutic effect of EC on gouty arthritis and its mechanisms.Methods and results: EC was added into a monosodium urate (MSU)-stimulated THP-1 cell that was induced by phorbol 12-myristate 13-acetate and lipopolysaccharide (LPS) in advance to establish a gout model in vitro. The efficiency of EC on acute gouty arthritis mice induced by MSU was further investigated. The results showed that EC concentration-dependently improved the cell viability of LPS and MSU stimulated THP-1 cells, and significantly alleviated MSU-induced ankle edema in mice in a dose-dependent manner. In addition, EC inhibited the infiltration of inflammatory cells and local cascular congestion in ankle joint tissue. Furthermore, the secretion of inflammatory cytokines (IL-1β, IL-18, IL-6, and TNF-α) activation of NLRP3 inflammasome and NF-κB signaling pathway were markedly suppressed by EC in vitro and in vivo.Conclusion: These results indicated that EC could effectively improve MSU-induced acute gouty arthritis via inhibiting NLRP3 inflammasome and the NF-κB signaling pathway in vitro and in vivo, which suggested that EC might be a promising active ingredient for the prevention and treatment of gouty arthritis.

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“…Furthermore, epicatechin from cocoa beans improves physical performance consistently with the modulation of biomarkers of sarcopenia by decreasing FoxO1A and MuRF1, regulators of muscle degradation [ 83 ]. Epicatechin has also been shown to have anti-inflammatory effects in diverse cell types, such as hepatic and glial cells, but this effect remains to be proved in sarcopenia [ 117 , 118 ].…”

Section: Other Therapeutic Approaches For Sarcopeniamentioning

confidence: 99%

Molecular Mechanisms of Inflammation in Sarcopenia: Diagnosis and Therapeutic Update

Jiménez-Gutiérrez

Martínez-Gómez

Martínez-Armenta

et al. 2022

Cells

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Sarcopenia is generally an age-related condition that directly impacts the quality of life. It is also related to chronic diseases such as metabolic dysfunction associated with diabetes and obesity. This means that everyone will be vulnerable to sarcopenia at some point in their life. Research to find the precise molecular mechanisms implicated in this condition can increase knowledge for the better prevention, diagnosis, and treatment of sarcopenia. Our work gathered the most recent research regarding inflammation in sarcopenia and new therapeutic agents proposed to target its consequences in pyroptosis and cellular senescence. Finally, we compared dual X-ray absorptiometry (DXA), magnetic resonance imaging (MRI), and ultrasound (US) as imaging techniques to diagnose and follow up on sarcopenia, indicating their respective advantages and disadvantages. Our goal is for the scientific evidence presented here to help guide future research to understand the molecular mechanisms involved in sarcopenia, new treatment strategies, and their translation into clinical practice.

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Protective effect of Epicatechin on APAP-induced acute liver injury of mice through anti-inflammation and apoptosis inhibition

Cited by 21 publications

References 11 publications

Antioxidant and Anti-Inflammatory Activities of Six Flavonoids from Smilax glabra Roxb

Antioxidant and Anti-Inflammatory Activities of Six Flavonoids from Smilax glabra Roxb

(−)-Epicatechin Ameliorates Monosodium Urate-Induced Acute Gouty Arthritis Through Inhibiting NLRP3 Inflammasome and the NF-κB Signaling Pathway

Molecular Mechanisms of Inflammation in Sarcopenia: Diagnosis and Therapeutic Update

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