Protective effect of hypothermia and vitamin E on spermatogenic function after reduction of testicular torsion in rats

Abstract

This study was designed to investigate the protective effect of hypothermia and vitamin E on spermatogenic function after reduction of testicular torsion in rats. Ninety-six pure inbred male SD rats were divided into group A, B, C and D according to the principle of body weight and birth similarity, with 24 rats in each group. Four groups of rats were respectively twisted on the left testis to establish unilateral testicular torsion rats. Rats in groups A, B, C, D were respectively given normal saline, hypothermia therapy, vitamin E therapy, and hypothermia and vitamin E therapy. The superoxide dismutase (SOD) activity and malondialdehyde (MDA) content of the four groups were detected, and the correlation levels of inflammatory factors IL-1β, hs-CRP and related sex hormones luteinizing hormone (LH), follicle-stimulating hormone (FSH), total testosterone (T) were detected by ELISA. Apoptosis of spermatogenic cells of testis in the four groups was detected by flow cytometry. SOD activity and MDA content in groups B, C and D were significantly higher than those in group A, MDA content was significantly lower than that in group A (P<0.05), SOD activity in group D was higher than that in groups B and C, while MDA content was lower than that in groups B and C (P<0.05). The levels of IL-1β and hs-CRP in group A were much higher than those in groups B, C and D (P<0.05). LH and FSH levels in group A were significantly higher than those in groups B, C and D (P<0.05), and in group D were significantly lower than those in groups B and C (P<0.05). Apoptosis rate of spermatogenic cells in group A was significantly higher than that in groups B, C and D (P<0.05). Hypothermia combined with vitamin E can reverse testicular injury in rats and reduce the apoptosis rate of spermatogenic cells.

Vitamin E ameliorates disturbances in testosterone pathway and sperm quality of male rats induced by the glycosides vicine and convicine of Vicia faba

“…In the current study; the injection with V and C induced a decline in serum Ts and DHEA-SO 4 levels in the rats, where the major part of male reproductive system is dependent on Ts; the process of spermatogenesis will eventually cease where vitamin E support in the absence of this hormone (Anvari et al, 2020;Sadeghi et al, 2020;Bo et al, 2020). The decrease of Ts may be attributed to: V and C inhibit human chronic gonadotropin-stimulated Ts biosynthesis by both cultured rat precursor and immature leydig cells (Pasqualotto et al, 2003); or the effect of V and C, which localized between enzymatic steps of Ts and DHEA-SO 4 biosynthesis, changing cholesterol to androstenedione through inhibiting 17a-hydroxylase, cholesterol side chain cleavage and 3b HSD-isomerase steps (which change cholesterol to pregnenolone and pregnenolone to progesterone, respectively (Saradha and Mathur, 2006).…”

“…The effect of vitamin E might be through the scavenging of reactive oxygen species induced by V and C (Diemer et al, 2003). Vitamin E reverses testicular injury in rats and declines the apoptosis rate of spermatogenic cells (Bo et al, 2020). Also, vitamin E prevents coal burning fluorosis-induced spermatogenic cell apoptosis through the suppression of oxidative stress (Tian et al, 2018).…”

Pathophysiology and management of testicular ischemia/reperfusion injury: Lessons from animal models

“… [ 27 ] Rat model Suture 2 h Low midline laparotomy 30 days HBO 1 and 12 h post-ischeamia and once daily for 30 days 100 % oxygen for 1 h at the pressure of 2.4 atm for 30 days HBO ameliorated I/R-induced testicular toxicity by preventing oxidative stress, inflammatory response, and reducing NO formation [ 269 ] Rat model Clamp 4 h Inguinal incisions. 2 weeks HBO 150 min post-ischeamia and immediately after ischeamia 90 min treatment at 2.5 atm with 100 % oxygen HBO prevented I/R-induced testicular injury by preserving the germinal epithelium Hypothermia [ 270 ] Rat model Clamp 4 h Scrotum 1 h Hypothermia 5s, 10s intermittent reperfusion 4 0 c Hypothermia reversed I/R-induced ↓MDA, MPO [ 271 ] Rat model Suture 4 h Scrotum 4 h Pentoxifylline, Hypothermia 30 min pre detorsion 40 mg/kg Hypothermia reversed I/R-induced ↓aperm count and testosterone↓MDA↓Bcl 2, caspase 3 and Bax [ 272 ] Rat model Suture 2 h Scrotum 2 h Hypothermia And vit E 30 min, 90min and 30 min befor...…”

THE POTENTIAL OF TOMATO EXTRACT (Lycopersicum esculentum L.) IN INCREASING THE SERTOLI CELLS AND SPERMATOGENIC CELLS COUNT OF THE CONTRALATERAL TESTICULAR SEMINIFEROUS TUBULES OF RATS (Rattus norvegicus) THAT HAVE TESTICULAR TORSION

“…Induction of testicular torsion was carried out according to the model of testicular torsion rats developed by Ranade et al (2011) and Bo et al (2020). After the grouping, rats in the NC group underwent sham/placebo surgery, while those in the PC, T1, and T2 groups were anesthetized with a ketamine injection (Ilium, NSW, 100 mg ketamine/mL) at a dose of 10 mg/kg BW and xylazine (Ilium, NSW, 20 mg xylazine/mL) dose of 2 mg/kg BW.…”