Protective effect of hydrogen sulfide on experimental testicular ischemia reperfusion in rats

Bozkurt, Muammer; Değirmentepe, Recep Burak; Polat, Emre Can; Yıldırım, Funda; Sönmez, Kıvılcım; Çekmen, Mustafa; Eraldemır, Ceyla; Ötünçtemur, Alper

doi:10.1016/j.jpurol.2019.10.006

Cited by 21 publications

(12 citation statements)

References 28 publications

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“…Testicular damage caused by torsion and detorsion is regarded as a classical ischemia-reperfusion injury. Testicular ischemia-reperfusion causes overproduction of reactive oxygen species [ 9 – 12 ]. Excessive reactive oxygen species have a destructive effect on cells by inducing peroxidation of cellular membrane lipids, DNA disintegration, and protein denaturation [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…Under normal conditions, reactive oxygen species at low concentration are indispensable for physiological processes, such as killing bacteria, cell differentiation, activating transcription factors in signal transduction pathways, cell proliferation, apoptosis, and protein phosphorylation [ 49 – 56 ]. However, under pathological conditions, such as tissular ischemia-reperfusion, excessive reactive oxygen species are produced [ 9 – 12 ]. Reactive oxygen species at high concentration can destroy cellular macromolecules, including lipids, proteins, and nucleic acids, and lead to cellular damage [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…Testicular damage after torsion-detorsion is thought to be a typical ischemia-reperfusion injury. Tissular ischemia-reperfusion causes production of large quantities of reactive oxygen species [ 9 – 12 ]. Reactive oxygen species, including superoxide anion, nitric oxide, peroxynitrite, hydrogen peroxide, and hydroxyl radical, can result in tissue damage by inducing lipid peroxidation in the cellular and mitochondrial membranes, DNA destruction, and protein denaturation [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

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Salidroside Exerts Beneficial Effect on Testicular Ischemia-Reperfusion Injury in Rats

Wei

Huang

Qin

2022

Oxidative Medicine and Cellular Longevity

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Testicular torsion-detorsion results in testicular ischemia-reperfusion injury, which is associated with overgeneration of reactive oxygen species. Salidroside, a major bioactive ingredient extracted from Rhodiola rosea, has strong antioxidant activity. The purpose of this study was to examine the effect of salidroside on testicular ischemia-reperfusion injury. Sixty rats were randomly separated into 3 experimental groups: group A = sham-operated control; group B = testicular ischemia-reperfusion; and group C = testicular ischemia-reperfusion treated with salidroside. The rats in the sham-operated control group received all surgical procedures except testicular torsion-detorsion. The testicular ischemia-reperfusion group underwent 2 hours of left testicular torsion followed by detorsion. The rats in the salidroside-treated group received the same surgical procedure as in testicular ischemia-reperfusion group, but salidroside was injected intraperitoneally at reperfusion. Testicular malondialdehyde content (a reliable index of reactive oxygen species) and protein expression of superoxide dismutase and catalase which are primary antioxidant enzymes in testes were measured at 4 hours after reperfusion. Testicular spermatogenesis was evaluated at 3 months after reperfusion. The malondialdehyde content increased significantly, while superoxide dismutase and catalase protein expression and testicular spermatogenesis reduced significantly in ipsilateral testes of testicular ischemia-reperfusion group, as compared with sham-operated control group. Therapy with salidroside significantly reduced malondialdehyde content and significantly enhanced superoxide dismutase and catalase protein expression and spermatogenesis in ipsilateral testes, as compared with testicular ischemia-reperfusion group. The present findings indicate that treatment with salidroside ameliorates testicular ischemia-reperfusion injury by reducing reactive oxygen species level by upregulating superoxide dismutase and catalase protein expression.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Salidroside Exerts Beneficial Effect on Testicular Ischemia-Reperfusion Injury in Rats

Wei

Huang

Qin

2022

Oxidative Medicine and Cellular Longevity

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“…The primary pathological mechanism of testicular torsion is ischemia-reperfusion (I/R) injury. Changes in microvascular blood ow cause the release of proin ammatory cytokines and production of reactive oxygen species (ROS) [5]. This leads to disorganization of the spermatogenic cell structure and function, or even apoptosis, leading to spermatogenic impairment [6,7].…”

Section: Introductionmentioning

confidence: 99%

Adipose-derived mesenchymal stromal cell-derived exosomes alleviate testicular torsion injury via activation of PI3K/AKT and MAPK/ERK1/2 pathways

Liu¹,

Shi²,

Li³

et al. 2021

Preprint

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Background Exosomes have became a new nano-scale therapeutic method in recent years. Adipose-derived mesenchymal stromal cell-derived exosomes (ADSC-Exos) have shown great potential in the treatment of ischemia-reperfusion injury. However, there is no research on whether ADSC-Exos can alleviate testicular torsion injury. Therefore, we investigated the protective effect of ADSC-Exos in alleviating testicular torsion-detorsion injury. Methods ADSC-Exos were isolated by ultracentrifugation and injected into the torsion-detorsion-affected testes of rats. H&E staining and sperm quality were used to evaluate the therapeutic effects of ADSC-Exos, and the level of tissue oxidative stress was determined using malondialdehyde (MDA) and superoxide dismutase (SOD). In addition, TUNEL staining and immunohistological analyses (Ki67, cleaved Caspase-3, IL-6, IL-10, CCR7, and CD163) were used to clarify the effects of ADSC-Exos on spermatogenic cell proliferation, apoptosis, and the inflammatory microenvironment in vivo . Possible signaling pathways were predicted using sequencing technology and bioinformatics analysis. The signaling pathways were validated by proliferation (EdU), migration (transwell and scratch test), and apoptosis (flow cytometry, TUNEL, and western blot) in vitro . Results ADSC-Exos alleviated testicular torsion-detorsion injury by attenuating oxidative stress and inflammatory responses. In addition, ADSC-Exos promoted the proliferation and migration of spermatogenic cells and inhibited their apoptosis by activating the PI3K/AKT and MAPK/ERK1/2 signaling pathways. Conclusions Overall, we demonstrated that ADSC-Exos can alleviate testicular torsion injury and provided a new approach for treating testicular torsion injury.

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“…However, histological evaluation is insufficient. It would be better if the study was supported by immunohistochemical parameters such as apoptotic protease-activating factor 1 (apaf-1) and inducible nitric oxide synthase (iNOS) (Bozkurt et al, 2020). Interestingly, the study did not mention the Johnsen scoring system, which determines spermatogenesis activity in testis (Johnsen, 1970).…”

mentioning

confidence: 99%

Letter to the editor regarding the article 'Investigation of the protective effect of nesfatin‐1 on testicular ischaemia‐reperfusion damage: An experimental study'

Değirmentepe

Bozkurt

2021

Andrologia

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Protective effect of hydrogen sulfide on experimental testicular ischemia reperfusion in rats

Cited by 21 publications

References 28 publications

Salidroside Exerts Beneficial Effect on Testicular Ischemia-Reperfusion Injury in Rats

Salidroside Exerts Beneficial Effect on Testicular Ischemia-Reperfusion Injury in Rats

Adipose-derived mesenchymal stromal cell-derived exosomes alleviate testicular torsion injury via activation of PI3K/AKT and MAPK/ERK1/2 pathways

Letter to the editor regarding the article 'Investigation of the protective effect of nesfatin‐1 on testicular ischaemia‐reperfusion damage: An experimental study'

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