Protective Effect of Hydroethanolic Extracts of Solanum scabrum and Cola verticillata Against Cyclophosphamide Induced Toxicity in Female Rats

Abstract

The aim of this study was to evaluate the protective effects of hydroethanolic extracts of S. scabrum and C. verticillata against cyclophosphamide induced toxicity. In this light, female albino wistar rats were treated by intraperitoneal administration of 100 mg/kg BW of cyclophosphamide or distilled water every other day for 7 days associated with oral gavage using hydroethanolic extract of C. verticillata/S. scabrum at a dose of 200 or 400 mg/kg BW or not every day for the same 7 days. On the 8th day, blood and organs (liver, heart and kidney) were collected for analyses of toxicity-related and oxidative stress markers. Cyclophosphamide treatment induced significant toxicity as shown by liver enzymes, urea and creatinine levels. The administration of extracts helped reduce the levels of these markers. The antioxidant effect of these extracts also helped or not to ameliorate oxidative stress markers (MDA, NO, hydroperoxides, catalase, thiols, GPx) depending on the extract and on the dose administered. These results suggest that administration of hydroethanolic extracts of S. scabrum and C. verticillata can help prevent or reduce toxicity that is brought about by treatment with cyclophosphamide due to their ability to upregulate antioxidant mechanisms.

AÇÃO PROFILÁTICA DO ISOTÔNICO FEITO A PARTIR DAS CASCAS DO FRUTO DA Oenocarpus bacaba Mart. (Arecaceae) CONTRA O ESTRESSE OXIDATIVO INDUZIDO POR CICLOFOSFAMIDA EM CAMUNDONGOS

“…No que tange os resultados obtidos, a CYP induziu a diminuição significativa na dose de 100 mg kg -1 (P < 0,05) na atividade da enzima da catalase no tecido hepático, isso porque o aumento das espécies reativas causados pela CYP demanda muito das enzimas antioxidantes, podendo acarretar na diminuição das mesmas (ANGIE et al, 2014). Igualmente foi observada a diminuição significativa (P < 0,05) do conteúdo da GSH no tecido hepático, substância esta reconhecida como o antioxidante mais afetado pelos metabólitos tóxicos da ciclofosfamida, pois liga-se à acroleína (metabólito gerado pela CYP) de forma irreversível, inativando-a (MOGHE et al, 2015).…”

Comparative Study of the Protective Effect of Cola anomala and Coffea arabica Against Induced Toxicity in Rats

“…In Asia, some decaffeinated extracts are thought to be responsible for their anti-inflammatory and chemopreventive properties through their action on androgenic receptors (Bureau, 2013). Studies carried out on Cola verticillata showed, antiproliferative and anti-toxic properties of this species (Mbong et al, 2013(Mbong et al, , 2014.…”