Protective Effect of Huangteng Soluble Powder on Endogenous Pyrogen and Central Mediators of Fever Induced by Lipopolysaccharide in Chicken

Abstract

The objective of this study was to understand the effect of Huangteng Soluble Powder (HTSP) on endogenous pyrogen and central mediators of fever induced by Lipopolysaccharide (LPS) in chicken. The model was established, and 90 chickens were randomly assigned into 6 groups, including Group I (control), Group II (injected with LPS), Group III (administered with Banqing Granules once a day for 3 days after 5 h post injection of LPS), Group IV, V and VI (administered with HTSP once a day for 3 days after 5 h post injection of LPS). The temperature was measured at different time periods, and samples were collected at 5 h post injection of LPS and 12 h, 24 h after the administration of HTSP. The results showed that HTSP significantly decreased the temperature of fever chicken as compared to Group II. The levels of IL-1, IL-6, TNF-α in serum and Prostaglandin E2 (PGE2), cyclic adenosine monophosphate (cAMP) in hypothalamus in Group II significantly increased at 5 h post injection of LPS and the mRNA expression levels of PGE2 and cAMP were also increased significantly in Group II as compared to control respectively. Furthermore as compared with Group II, the levels of IL-1, IL-6 in serum and PGE2, cAMP in the hypothalamus in Groups III, IV, V and VI decreased after 12 h and 24 h, the levels of TNF-α in serum in Group III and V decreased after 12 h and 24 h, and the mRNA expression levels of PGE2 and cAMP of Group V were significantly decreased at the end of the administration. In conclusion, HTSP can attenuate the LPS-induced fever by diminishing the levels of IL-1, IL-6 and TNF-α in serum and the levels of PGE2 and cAMP in the hypothalamus and inhibiting the mRNA expression of PGE2 and cAMP. , 2019. Protective effect of huangteng soluble powder on endogenous pyrogen and central mediators of fever induced by lipopolysaccharide in chicken. Pak Vet J, 39(2): 187-192. http://dx.