Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats

Wang, Chau‐Jong; Wang, Jinming; Lin, Wea‐Lung; Chu, Chia-Yih; Chou, Fen‐Pi; Tseng, Tsui‐Hwa

doi:10.1016/s0278-6915(00)00011-9

Cited by 305 publications

(221 citation statements)

References 22 publications

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“…26) There are two distinct pathways of t-BHP metabolism; one via cytochrome p-450, and the other by GSH-peroxidase converting t-BHP to t-butanol and oxidized GSH. 27,28) These metabolic pathways could increase cellular reactive metabolites, which may attack membrane phospholipids, proteins, and nucleic acids.…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Panduratin A against Oxidative Damage of tert-Butylhydroperoxide in Human HepG2 Cells

Sohn

Han

Lee

et al. 2005

Biological & Pharmaceutical Bulletin

105

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Reactive oxygen species (ROS) act as subcellular messengers in such complex cellular processes as mitogenic signal transduction, gene expression, and regulation of cell proliferation.1) Excessive production of ROS may, however, cause oxidative stress, loss of cell function, and ultimately apoptosis or necrosis.2) Fortunately, cells possess antioxidant molecules which can neutralize ROS before they induce damage on vital components. However, oxidative stress occurs when antioxidant systems are overwhelmed by ROS. Oxidative stress is considered to play a prominent role in the causation of many diseases including aging, cardiovascular disease, cancer and liver injury. 3,4) tert-Butylhydroperoxide (t-BHP) is an organic hydroperoxidant that can be metabolized to free radical intermediates, which can subsequently initiate lipid peroxidation, affect cell integrity, and form covalent bonds with cellular molecules resulting in cell injury. 5) t-BHP causes leakage of lactate dehydrogenase (LDH) and formation of malondialdehyde (MDA) in hepatocytes, 6) and also mediates DNA base damage in mammalian cells. 7) These phenomena are similar to the oxidative stress occurring in the cell and/or tissue.Because of its unique metabolism and relationship to the gastrointestinal tract, the liver is an important target of the toxicity of drugs, xenobiotics, and oxidative stress. 8) Reactive oxygen species derived from chemicals or drugs exposed to liver cells seem to mediate liver injury, although the mechanisms of free radical toxicity are not well understood. 9)Therefore, primary importance is to understand the role of antioxidants which can reduce the oxidative stress induced by reactive intermediates from various chemicals and drugs. We have investigated hepatoprotective drugs, based on the measurement of cytotoxicity of t-BHP against human-liver-derived HepG2 cells. HepG2 cells have been suggested to be a useful in vitro model for investigation of the metabolism and toxicity of drugs compared with animal cells, since HepG2 cells retain many of the specialized functions which are characteristics of normal human hepatocytes, including the synthesis and secretion of plasma proteins. 10)Kaempferia pandurata ROXB. is a perennial herb of the family Zingiberaceae cultivated in some tropical countries, including Thailand and Indonesia. Its rhizome has been used as a food ingredient, an aphrodisiac, and a folk medicine for the treatment of colic disorder, fungal infections, dry cough, rheumatism, and muscular pains.11,12) Panduratin A (Fig. 1A) is a chalcone derivative isolated from K. pandurata. It was recently reported that panduratin A possessed significant topical anti-inflammatory activity in murine macrophages, 12) and in the TPA-induced ear edema in rats. 13)The objective of the current study was to examine the ability of panduratin A to protect hepatocytic injury induced by t-BHP and to characterize the mechanisms involved. For this purpose, we have studied the cell viability, lipid peroxidation, glutathione (GSH) level, and ROS le...

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Section: Discussionmentioning

confidence: 99%

Protective Effects of Panduratin A against Oxidative Damage of tert-Butylhydroperoxide in Human HepG2 Cells

Sohn

Han

Lee

et al. 2005

Biological & Pharmaceutical Bulletin

105

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“…The calyces of the flower are consumed worldwide as a cold beverage and as a hot drink (sour tea). These extracts are also used in folk medicine against many complaints that include high blood pressure, liver diseases and fever (Dalziel 1937;Wang et al 2000;Ross 2003;Bako et al 2009). The red anthocyanin pigments in the calyces are used as food colouring agents (Esselen & Sammy 1975).…”

Section: Hibiscus Sabdariffa L Extractmentioning

confidence: 99%

“…Its flowers contain polyphenolic acid, flavonoids and anthocyanins (Chen et al 2003) with a considerable antioxidant activity (Tseng et al 1997;Wang et al 2000;Odigie et al 2003). There are numerous studies regarding HS components (Samy 1980;Rao 1996;Tsai et al 2002;Blazovics et al 2003) and their health-promoting properties.…”

Section: Hibiscus Sabdariffa L Extractmentioning

confidence: 99%

Role of food-derived antioxidant agents against acetaminophen-induced hepatotoxicity

Eugenio‐Pérez

Oca-Solano

Pedraza‐Chaverrí

2016

Pharmaceutical Biology

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Context Acetaminophen (APAP), also known as paracetamol and N-acetyl p-aminophenol, is one of the most frequently used drugs for analgesic and antipyretic purposes on a worldwide basis. It is safe and effective at recommended doses but has the potential for causing hepatotoxicity and acute liver failure (ALF) with overdose. To solve this problem, different strategies have been developed, including the use of compounds isolated from food, which have been studied to characterize their efficacy as natural dietary antioxidants. Objective The objective of this study is to show the beneficial effects of a variety of natural compounds and their use against acetaminophen-induced hepatotoxicity. Methods PubMed database was reviewed to compile data about natural compounds with hepatoprotective effects against APAP toxicity. Results and conclusion As a result, the health-promoting properties of 13 different food-derived compounds with protective effect against APAP-induced hepatotoxicity were described as well as the mechanisms involved in hepatoprotection.ARTICLE HISTORY

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“…These secondary metabolites of interests present in the petals may confer potent functional properties like free radicals scavenging and liver protection. [6][7][8] The liver is, indeed, a very important vital organ, due to the vital role it plays in various biochemical and physiological processes, notably the metabolic and detoxification functions. 9 The latter action of the liver is responsible for various diseases of this organ including hepatitis, liver cirrhosis, liver abscess, and liver cancer.…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective and antioxidant activities of Hibiscus sabdariffa petal extracts in Wistar rats

Obouayeba

Boyvin

M’boh

et al. 2014

Int J Basic Clin Pharmacol

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Background: Hibiscus sabdariffa is a medicinal plant rich in phytochemical compounds, which is the source of its biological properties. This study on the aqueous extract of H. sabdariffa (AEHS) was conducted to assess its hepatoprotective and antioxidant properties. Methods: It was carried out with 25 Wistar rats divided into five groups. Two groups were treated with a solution of NaCl 0.9%. One group was treated with silymarin at a dose of 25 mg/kg body weight (BW). Two other groups were treated with the AEHS at different doses (100 and 200 mg/kg BW). The treatments were carried out via oral route and at single dose for 7 days. After injection of 2,4-dinitrophenylhydrazine (DNPH), blood samples were collected for the carrying out of biochemical analyses of oxidative stress markers (thiobarbituric acid reactive substances, ferric reduction antioxidant parameter, and 2,2-diphenyl-1-picrylhydrazyl) and hepatotoxicity (albumin, total and direct bilirubin, alanine transaminase, aspartate aminotransferase, and lactate dehydrogenase). Results: Three major results were obtained. The hepatotoxicity of DNPH expressed by the rats of Group 1 was significantly different (p<0.05) from those of the other groups (control, 2-4) for both hepatotoxicity and oxidative stress markers. The hepatoprotective and antioxidant properties of the AEHS and confirmation of those of silymarin through the rats of Groups 2-4 were statistically identical (p<0.05) to the control group for markers of hepatotoxicity and oxidative stress. Conclusion: These results confirm and reinforce certain therapeutic virtues of H. sabdariffa.

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Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats

Cited by 305 publications

References 22 publications

Protective Effects of Panduratin A against Oxidative Damage of <i>tert</i>-Butylhydroperoxide in Human HepG2 Cells

Protective Effects of Panduratin A against Oxidative Damage of <i>tert</i>-Butylhydroperoxide in Human HepG2 Cells

Role of food-derived antioxidant agents against acetaminophen-induced hepatotoxicity

Hepatoprotective and antioxidant activities of <i>Hibiscus sabdariffa</i> petal extracts in Wistar rats

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Protective effect of Hibiscus anthocyanins against tert-butyl hydroperoxide-induced hepatic toxicity in rats

Cited by 305 publications

References 22 publications

Protective Effects of Panduratin A against Oxidative Damage of <i>tert</i>-Butylhydroperoxide in Human HepG2 Cells

Protective Effects of Panduratin A against Oxidative Damage of <i>tert</i>-Butylhydroperoxide in Human HepG2 Cells

Role of food-derived antioxidant agents against acetaminophen-induced hepatotoxicity

Hepatoprotective and antioxidant activities of &lt;i&gt;Hibiscus sabdariffa&lt;/i&gt; petal extracts in Wistar rats

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Hepatoprotective and antioxidant activities of <i>Hibiscus sabdariffa</i> petal extracts in Wistar rats