Protective effect of grape seed and skin extract on high dosage garlic-induced renal oxidative stress

Abstract

Protective effect of grape seed and Protective effect of grape seed and Protective effect of grape seed and skin extract on high dosage garlic skin extract on high dosage garlic skin extract on high dosage garlic---induced renal oxidative stress induced renal oxidative stress induced renal oxidative stress BJP IntroductionGarlic (Allium sativum L.) has been widely used in traditional medicine and its health effects are mainly attributable to organosulfur compounds (Berginc et al., 2010) and to flavonoids and phenolics (Shirzad et al., 2011). However, some controversies persist concerning garlic dose and mode of administration. In a prior study, we showed that a high garlic dose administered intraperitoneally (ip) induced a pro-oxidant effect and ultimately blood toxicity (Hamlaoui-Gasmi et al., 2011a).Grape seed and skin extract (GSSE) is a nutritional supplement exhibiting beneficial health effects (Suwannaphet et al., 2010) and containing polyphenolics classified as flavonoid and non-flavonoid compounds (Khanal et al., 2009). Flavonoids, which are highly concentrated in seeds, are mainly composed of monomeric catechins, proanthocyanidins, and flavonols such as quercetin (Renaud and De Lorgeril, 1992). Nonflavonoids, highly abundant in skin, contain stilbenes such as resveratrol, which is at the basis of the French Paradox (Renaud and De Lorgeril, 1992). GSSE has wide ranging benefits as cardioprotective (Decordé et al., 2009) and neuroprotective effects (Wang et al., 2009). More specifically, resveratrol is multi-organ protective owing to its anti-oxidant (Kelen and Tepe, 2007) and anti-inflammatory properties (Kowalczyk et al., 2010). Proanthocyanidins exert antineoplasic effects by inducing cytotoxicity towards some cancer cells (Chatelain et al., 2011). Quercetin and rutin are neuroprotective (Jimenez-Aliaga et al., 2011), and catechin, epicatechin, and gallic acid exhibited cholesterol-lowering activity (Ngamukote et al., 2011). AbstractIn this study, the protective role of grape seed and skin extract (GSSE) against high garlic dose-induced renal toxicity has been evaluated. Rats were intraperitoneally injected with garlic (5 g/kg bw) or GSSE (500 mg/kg bw) or a combination of garlic and GSSE at the same doses daily for one month. Renal oxidative stress markers and anti-oxidant status were evaluated. We also measured plasma creatinine and urea. Data showed that high garlic dose induced renal toxicity by increasing creatinine and urea and a pro-oxidative status characterized by increased malondialdehyde, carbonyl protein, calcium and H2O2, but decreased free iron. Unexpectedly garlic increased catalase but decreased peroxidase and superoxide dismutase activities. GSSE co-treatment counteracted almost all garlic-induced deleterious effects. In conclusion, high garlic dose induced a pro-oxidative state characterized by the Fenton reaction between H2O2 and free iron, inducing Ca 2+ depletion, while GSSE exerted anti -oxidant properties and Ca 2+ repletion. Article Info

Proanthocyanidin Protects Human Embryo Hepatocytes from Fluoride-Induced Oxidative Stress by Regulating Iron Metabolism

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Antihyperlipidemic and renoprotective activities of methanolic extract of <i>Canscora decussata</i> extract in alloxan-induced diabetic rabbits

“…Ipomoea staphylina leaves , Sechium eduleon , Nigella sativaon (Begum et al, 2006), Cinnamomum zeylanicum (Ullah et al, 2013), and grape seed and skin (Hamlaoui et al, 2012) extract shows renoprotective effect.…”

Grape Seed and Skin Extract Mitigates High Dosage Garlic-Induced Oxidative Stress in Rat Heart

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