Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: a light microscopic evaluation

Rashidian, Amir; Mehrzadi, Saeed; Ghannadi, Alireza; Mahzooni, Parvin; Sadr, Samira; Minaiyan, Mohsen

doi:10.1016/s2095-4964(14)60011-x

Cited by 49 publications

(23 citation statements)

References 20 publications

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“…45 The weight of the inflamed colonic tissue is considered as a reliable and sensitive indicator for the severity, extent of intestinal inflammation, over production of TNF α, IL-1β and IL-6 on intrarectal administration of acetic acid. 46,47,48,49 Consistent with this notion, the results of this study revealed, acetic acid treated rats was showed significant increase colon weight and macroscopic damage score, indicative of formation of ulcers, edema and increases inflammatory cytokines levels such as TNF α, IL-1β and IL-6. Pretreatment with TRP 1 in acetic acid induced colitis significantly reduced the weight of inflamed colon weight, macroscopic damage compare with colitis rats and reverse elevated TNF α, IL-1β and IL-6 levels and prevent epithelial damage indicating its protective effect from ulcerative colitis.…”

Section: Discussionsupporting

confidence: 85%

Protective Effect of A2B Receptor Antagonist (TRP 1) on Acetic Acid Induced Ulcerative Colitis in Rats: in vitro, in vivo and in silico Methods

Pasala¹,

Alluri²,

Mavulati³

et al. 2018

IJPER

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Aim: Present study was elucidate the protective effect of pyridinone derivatives such as 7-amino-5-oxo-2-Phenyl-5H, 8H-dihydro- [1,2,4] triazolo [1, 5-α] pyridine -6carbonitril (TRP 1) by in vitro, in vivo and in silico. Methods: Radioligand binding assay was performed on human adenosine receptors (A 2B ) and assess A 2B antagonist effect by adenylyl cyclase activity. In vitro study was carried out to determine the neutralize capacity against DPPH*, NO*, SO*, LPO* free radicals. TRP 1 at the doses 1 mg/ kg bd.wt. and 10 mg/kg bd.wt p.o, was administered consecutively for 14 days in albino rats. Ulcerative colitis was induced with single dose of 2 ml of 3% acetic acid intrarectal on 14 th day in treated rats. At the end of treatment, colonic tissue was collected and subjected for estimation of macroscopic score, glutathione, catalase, MPO and inflammatory parameters such as IL 1β, TNF α and IL 6. In silico study was carried out to evaluate the binding energy and IC 50 toward IL 1β, TNF α and IL 6. Results: TRP 1 was antagonized the A 2B receptors at the concentration of 30000 nM. In vitro study was revealed that TRP1 (1 mg/ml) was significantly neutralizes the free radicals of DPPH*, SO*, NO*and LPO*. In in vivo studies, intrarectal administration of acetic acid caused significantly (***P<0.001) increased macroscopic score, colon weight, colonic MPO, IL 6, IL 1β and TNF-α (*P<0.05), while TRP 1 treated colitis rats antioxidants system such as GSH (**P<0.01), catalase (*P<0.05) activity was significantly improved, decreases inflammatory mediators such TNF α (*P<0.05), IL 1β (**P<0.01) , IL 6 (**P< 0.01) and also suppresses the MPO activity (*P<0.05). In silico study was reported that the IC 50 of TPR 1 against IL 1β, IL 6 and TNF-α was 7.5 mM, 28.65 mM and 45.87 mM respectively. Conclusion: Our data demonstrated that the TRP 1 treatment improved clinical score in acetic acid induced colitis in rats. It also inhibited the proinflammatory cytokine IL-6, IL 1β and TNF α and improvements of antioxidant in colitis rats through A 2B receptor antagonist property.

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Section: Discussionsupporting

confidence: 85%

Protective Effect of A2B Receptor Antagonist (TRP 1) on Acetic Acid Induced Ulcerative Colitis in Rats: in vitro, in vivo and in silico Methods

Pasala¹,

Alluri²,

Mavulati³

et al. 2018

IJPER

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“…Haematoxylin and eosin staining was performed using paraffin sections. The histological score (HS) was determined by microscopically under a light microscope (Olympus Corp., Tokyo, Japan) examining stained paraffin sections using the scoring criteria of colonic histological activity index (21–23). Each sample was randomly selected from five perspectives to calculate the total score (0–10 points), including the infiltration degree of inflammatory cells, ulcers and lesion level (Table I).…”

Section: Methodsmentioning

confidence: 99%

Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate-induced ulcerative colitis

Wang

Liu

Guo

et al. 2017

Experimental and Therapeutic Medicine

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The effects of indigo naturalis (IN), which is a traditional Chinese herbal formulation, have been clinically demonstrated in treating refractory ulcerative colitis (UC). The present study aimed to verify the effects and mechanisms of IN in experimental UC rats. A total of 48 male Sprague-Dawley rats were randomly divided into six groups: Chow, model, high-dose IN, medium-dose IN, low-dose IN and mesalazine (a bowel-specific aminosalicylate drug) groups. The models were administered 3.5% dextran sodium sulphate solution for 7 days. The treatment groups were administered IN or mesalazine and then sacrificed and sampled on day 8. Disease activity index (DAI), histological damage score (HDS) and myeloperoxidase (MPO) activity were used to evaluate the severity of UC. Colon and serum cytokines were detected using liquid-phase chip technology and the expression of occludin protein in colonic mucosa was assessed by immunohistochemistry and western blot analysis. The results indicated that the oral administration of IN may reduce DAI, HDS and MPO activity. IN also reduced the expression of inflammatory cytokines and increased the expression of colonic mucosal repair-related cytokines and occludin protein. These results highlight the potential of IN as a therapeutic agent for treating UC through its action of inflammation control and colonic mucosal damage repair.

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“…Ginger essential oil (rich in ar-curcumene) has also shown anti-inflammatory properties through affecting leukocyte migration both in vitro and in vivo (de Melo et al, 2011). The volatile oil of Zingiber officinale with zingiberene, ar-curcumene, β-bisabolene and α-sesquiphellandrene as the major components, has shown to be effective in decreasing severity and extent of inflammation in a rat model of colitis (Rashidian et al, 2014). In another study, evaluating the volatile oil of ginger (with zingiberene as the dominant composition) in vitro and in vivo has suggested the impact of the oil in cell-mediated immune response and nonspecific proliferation of T-cell which might play a role in inflammatory conditions (Zhou et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Volatile Constituents of Ginger Oil Prepared According to Iranian Traditional Medicine and Conventional Method: A Comparative Study

Shirooye¹,

Mokaberinejad²,

Ara³

et al. 2016

Afr J Tradit Complement Altern Med

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Background: Herbal medicines formulated as oils were believed to possess more powerful effects than their original plants in Iranian Traditional Medicine (ITM). One of the popular oils suggested for treatment of various indications was ginger oil. In the present study, to suggest a more convenient method of oil preparation (compared to the traditional method), ginger oil has been prepared according to both the traditional and conventional maceration methods and the volatile oil constituents have been compared. Material and Methods: Ginger oil was obtained in sesame oil according to both the traditional way and the conventional (maceration) methods. The volatile oil of dried ginger and both oils were obtained by hydro-distillation and analyzed by gas chromatography/mass spectroscopy. Results: Fifty five, fifty nine and fifty one components consisting 94 %, 94 % and 98 % of the total compounds were identified in the volatile oil of ginger, traditional and conventional oils, respectively. Conclusion:The most dominant compounds of the traditional and conventional oils were almost similar; however they were different from ginger essential oil which has also been to possess limited amounts of anti-inflammatory components. It was concluded that ginger oil could be prepared through maceration method and used for indications mentioned in ITM.

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Protective effect of ginger volatile oil against acetic acid-induced colitis in rats: a light microscopic evaluation

Cited by 49 publications

References 20 publications

Protective Effect of A2B Receptor Antagonist (TRP 1) on Acetic Acid Induced Ulcerative Colitis in Rats: in vitro, in vivo and in silico Methods

Protective Effect of A2B Receptor Antagonist (TRP 1) on Acetic Acid Induced Ulcerative Colitis in Rats: in vitro, in vivo and in silico Methods

Effects of indigo naturalis on colonic mucosal injuries and inflammation in rats with dextran sodium sulphate-induced ulcerative colitis

Volatile Constituents of Ginger Oil Prepared According to Iranian Traditional Medicine and Conventional Method: A Comparative Study

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