Protective effect of ghrelin on testicular damages caused by chronic hypoxia in rats: A histopathological study

Hazrati, Abdullah; Salimnejad, Ramin; Alipour, Mohammad Reza; Bavil, Fariba Mirzaei; Alihemmati, Alireza

doi:10.1111/and.12989

Cited by 14 publications

(7 citation statements)

References 27 publications

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“…It has been established that obestatin plays a role in increasing cell survival and proliferation and counteracting apoptotic process and inflammation [41]. Regarding the reproductive system, a protective activity of ghrelin was observed in different researches, performed in animal models, demonstrating that this peptide can decrease the testicular damages [17, 42–44]. Recent studies deal with the defensive role of ghrelin that can exert beneficial activity on redox imbalance for its anti-inflammatory and antioxidant properties also in the male reproductive system [18, 45–47].…”

Section: Discussionmentioning

confidence: 99%

Relationships between Ghrelin and Obestatin with MDA, Proinflammatory Cytokines, GSH/GSSG Ratio, Catalase Activity, and Semen Parameters in Infertile Patients with Leukocytospermia and Varicocele

Micheli

Collodel

Cerretani

et al. 2019

Oxidative Medicine and Cellular Longevity

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Ghrelin and obestatin are involved in many biological functions including reproduction. Growing evidences suggest that both peptides could exert protective and antioxidant activities. In this study, the relationships between ghrelin/obestatin, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA), reduced glutathione (GSH), oxidized glutathione (GSSG), expressed as the GSH/GSSG ratio, catalase (CAT), and semen parameters in infertile patients with varicocele or leukocytospermia and controls were investigated. Fifty-six infertile patients (32 with leukocytospermia and 24 with varicocele) and 14 controls participated in this study. Semen analysis was performed following the WHO guidelines. Apoptotic and necrotic sperm were scored by annexin V/propidium iodide assay. Seminal plasma samples were used for the following determinations: ghrelin, obestatin, IL-6, and TNF-α were measured by an immunological method, GSH/GSSG by an enzymatic method, and CAT by spectrophotometric determination. With respect to controls, both the leukocytospermia and varicocele groups showed altered sperm parameters, significantly increased sperm apoptosis (P = 0.009 and P = 0.011, respectively), IL-6 (P = 0.0001 and P = 0.004, respectively), and TNF-α levels (P = 0.0001 and P = 0.002, respectively); both groups had significantly decreased levels of ghrelin (P = 0.0001), obestatin (P = 0.0001 and P = 0.006, respectively), and GSH/GSSG ratio (P = 0.003 and P = 0.0001, respectively). The MDA concentration was significantly increased in the leukocytospermia group vs. controls (P = 0.0001), in the varicocele group vs. controls (P = 0.011), and in the leukocytospermia group vs. the varicocele group (P = 0.008). CAT activity was augmented in both the leukocytospermia and varicocele groups (P = 0.0001)vs. controls. The results indicate that both ghrelin and obestatin may play a protective role in human semen and this effect is probably due to their antioxidant properties.

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Section: Discussionmentioning

confidence: 99%

Relationships between Ghrelin and Obestatin with MDA, Proinflammatory Cytokines, GSH/GSSG Ratio, Catalase Activity, and Semen Parameters in Infertile Patients with Leukocytospermia and Varicocele

Micheli

Collodel

Cerretani

et al. 2019

Oxidative Medicine and Cellular Longevity

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“…PAS staining was used to evaluate glycogen content in (glycogen storage) in hepatocytes. Finally, to evaluate the tissue changes in each sample, 12 images were randomly selected then the histological index was calculated using semiquantitative method described by Frei et al The level of damage was recorded based on (0-3) grades in which grade 0 = no damage, 1 = mild damage, 2 = moderatedamage, and 3 = severe damage (20)(21)(22)(23).Slides were viewed and photographed using a camera microscope at 400x magnification in at least three random microscopic fields from each animal by two expert pathologists without knowledge of the treatment groups [22][23][24][25].…”

Section: Evaluation Of Histopathological Alterationsmentioning

confidence: 99%

Hepatoprotective Impact of Ghrelin against Cyclophosphamide-Induced Toxicity in the Male Mice

et al. 2021

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Background Despite its vast spectrum of clinical usage, cyclophosphamide (CP) exerts many adverse impacts, including hepatotoxicity. Antioxidant properties of ghrelin might protect the liver from CP-induced toxicity. The current study aimed to assess the protective impacts of ghrelin on CP-induced liver toxicity. Methods Forty male mice were randomly divided into four groups (n=10) Group 1 as control received no intervention,group 2 received cyclophosphamide (CP) (100 mg/kg, i.p.) for five weeks and once a week. Group 3 received CP+ghrelin (CP+G), (80 µg/kg daily, i.p.) for five weeks. Group 4 received ghrelin with above-mentioned dose. At the end of the experiment, the mice were sacrificed to remove liver tissuesfor histological and biochemical examination. Results Malondialdehyde (MDA) level increased after CP treatment but ghrelin administration significantly decreased the level of MDA (P<0.05). Measurement of the total antioxidant capacity (TAC) noted a significant decrease in the CP group against the control group (P<0.05). Ghrelin treatment in the CP+G group considerably increased the TAC activity when compared to the CP group (P<0.05). Histological examinations also confirmed the hepatocyte necrosis, local bleeding and inflammation, vacuolation, and sinusoidal dilation in the CP group, ghrelin administration reduced the destructive effects of CP on the liver significantly (P<0.05). Conclusion Our results reveal the hepatoprotective effect of ghrelin against CP. Therefore, ghrelin might be useful in protecting the body against the adverse impacts of injuries induced by chemotherapeutic drugs.

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“…The morphometric analysis was performed to calculate seminiferous tubules' diameter and the height of the germinal epithelium (HE) in seminiferous tubules. Besides, the thickness of the basement membrane of seminiferous tubules was measured using PAS staining (periodic acid-Schiff) (Hazrati, Salimnejad, Alipour, Mirzaei Bavil, & Alihemmati, 2018). The histopathological alterations were assessed semi-quantitatively with the use of a four-point scale:…”

Section: Histopathological Evaluationsmentioning

confidence: 99%

Fumaria parviflora regulates oxidative stress and apoptosis gene expression in the rat model of varicocele induction

et al. 2020

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Varicocele is one of the leading causes of male infertility in which oxidative stress induces DNA damages in spermatozoa of patients with varicocele. Recent studies indicated that the treatment with antioxidant agents has protective effects against the formation of reactive oxygen species (ROS). Our research aimed to evaluate the impact of Fumaria Parviflora (FP) on the varicocele‐induced testicular injury. For this purpose, 32 adult male Wistar rats (n = 8 per group) were randomly assigned to four groups as follows: sham group, varicocele group, varicocele treatment group and the control treatment group. The experimental groups daily received FP (250 mg/kg) for 8 weeks. The induction of varicocele was conducted by partial occlusion on the left renal vein. The diameter of seminiferous tubules, Johnsen's score and the epithelium thickness improved in the treated‐varicocele group as compared to the varicocele group. FP extract could increase the biochemical parameters including superoxide dismutase and glutathione peroxidase, and also decrease malondialdehyde level in the varicocele group. Furthermore, varicocele markedly increased both mRNA and intensity of Bax, while treatment with FP could alleviate them. We concluded that FP could alleviate varicocele, possibly by lowering oxidative stress and testicular damage.

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Protective effect of ghrelin on testicular damages caused by chronic hypoxia in rats: A histopathological study

Cited by 14 publications

References 27 publications

Relationships between Ghrelin and Obestatin with MDA, Proinflammatory Cytokines, GSH/GSSG Ratio, Catalase Activity, and Semen Parameters in Infertile Patients with Leukocytospermia and Varicocele

Relationships between Ghrelin and Obestatin with MDA, Proinflammatory Cytokines, GSH/GSSG Ratio, Catalase Activity, and Semen Parameters in Infertile Patients with Leukocytospermia and Varicocele

Hepatoprotective Impact of Ghrelin against Cyclophosphamide-Induced Toxicity in the Male Mice

Fumaria parviflora regulates oxidative stress and apoptosis gene expression in the rat model of varicocele induction

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