Protective effect of genkwanin against lipopolysaccharide-induced acute lung injury in mice with p38 mitogen-activated protein kinase and nuclear factor-κB pathway inhibition

Ni, Yung-Lun; Shen, Huan-Ting; Chen, Shih‐Pin; Kuan, Yu‐Hsiang

doi:10.1016/j.jff.2022.105271

Cited by 6 publications

(9 citation statements)

References 31 publications

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“…determinant of the condition's severity. 18 As illustrated in Figure 3, lung edema was significantly induced by LPS in ALI mice compared to the control group. Conversely, the LPS-induced lung edema was effectively ameliorated through treatment with hibifolin at concentrations of 30 and 50 mg/kg and dexamethasone.…”

Section: Pretreatment With Hibifolin Alleviated the Pulmonary Edema I...mentioning

confidence: 89%

“…This included the assessment of neutrophil infiltration or aggregation within both the alveolar space and lung interstitium, as well as the examination of alveolar and pulmonary capillary congestion, hemorrhaging, thickening of alveolar walls, and the formation of hyaline membranes. 1,3,18 As illustrated in Figure 1, the lung tissue slices from mice exposed to LPS exhibited substantial and distinct alterations in pathological characteristics compared to the control group. Conversely, the LPS-induced changes in these pathological characteristics were effectively ameliorated through treatment with hibifolin at concentrations of 30 and 50 mg/kg and dexamethasone (Figure 2).…”

Section: Pretreatment With Hibifolin Alleviated the Histopathologic P...mentioning

confidence: 99%

“…administration of 100 μg/50 μL of LPS. Subsequently, the mice were anesthetized with sodium pentobarbital, BALF, and lung tissues collected after 6 h. 18,19…”

Section: Animal Protocol and Experimental Designmentioning

confidence: 99%

“…After euthanizing the mice, the lung tissues were collected, ensuring the removal of any excess moisture and blood from their surfaces. 18 The wet weight of the lungs was immediately ascertained following the sample extraction, utilizing a precision electronic balance. Subsequently, the lung tissues were carefully wrapped in aluminum foil and subjected to 72 h of desiccation in an 80 C oven to achieve complete dryness.…”

Section: Detection Of Lung Wet-to-dry Weight Ratiomentioning

confidence: 99%

“…Protein extraction from pulmonary tissue was achieved by homogenization in T-Per tissue protein extraction reagent (Thermo Fisher Scientific, Rockford, USA) supplemented with protease and phosphatase inhibitor cocktails (Sigma-Aldrich, MO, USA), all procedures were conducted on ice to prevent degradation. 18,21 The homogenates were then centrifugated at 12 000 rpm for 10 min at 4 C. Proteins, at a concentration of 50 μg/mL, were resolved on a 10% sodium dodecyl sulfate-polyacrylamide gel within a Tris-Glycine-sodium dodecyl sulfate running buffer. The resolved proteins were subsequently electrotransferred onto PVDF membranes (Bio-Rad Laboratories, CA, USA).…”

Section: Western Blot Assay In Lungsmentioning

confidence: 99%

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Hibifolin protected pro‐inflammatory response and oxidative stress in LPS‐induced acute lung injury through antioxidative enzymes and the AMPK2/Nrf‐2 pathway

Ni,

Shen,

et al. 2024

Environmental Toxicology

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ALI is a grave medical ailment that manifests as abrupt inflammation of the lungs and diminished oxygen levels. It poses a considerable challenge to the medical fraternity, with elevated rates of morbidity and mortality. Our research endeavors to investigate the potential of hibifolin, a flavonoid glucuronide, imbued with potent antioxidant properties, and its molecular mechanism to combat LPS‐induced ALI in mice. The study utilized ICR mice to create an ALI model induced by LPS. Prior to LPS administration, hibifolin was given at 10, 30, or 50 mg/kg, or dexamethasone was given at 1 mg/kg to assess its preventative impact. Changes in lung tissue, pulmonary edema, and lipid peroxidation were analyzed using H&E stain assay, lung wet/dry ratio assay, and MDA formation assay, respectively. Activity assay kits were used to measure MPO activity and antioxidative enzymes (SOD, CAT, GPx) activity in the lungs. Western blot assay was used to determine the phosphorylation of Nrf‐2 and AMPK2 in the lungs. Hibifolin demonstrated a concentration‐dependent improvement in LPS‐induced histopathologic pulmonary changes. This treatment notably mitigated pulmonary edema, lipid peroxidation, and MPO activity in ALI mice. Additionally, hibifolin successfully restored antioxidative enzyme activity in the lungs of ALI mice. Moreover, hibifolin effectively promoted Nrf‐2 phosphorylation and reinstated AMPK2 phosphorylation in the lungs of ALI mice. The results indicate that hibifolin could effectively alleviate the pathophysiological impact of LPS‐induced ALI. This is likely due to its antioxidative properties, which help to restore antioxidative enzyme activity and activate the AMPK2/Nrf2 pathway. These findings are valuable in terms of enhancing our knowledge of ALI treatment and pave the way for further investigation into hibifolin as a potential therapeutic option for lung injuries.

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Section: Pretreatment With Hibifolin Alleviated the Pulmonary Edema I...mentioning

confidence: 89%

Section: Pretreatment With Hibifolin Alleviated the Histopathologic P...mentioning

confidence: 99%

“…administration of 100 μg/50 μL of LPS. Subsequently, the mice were anesthetized with sodium pentobarbital, BALF, and lung tissues collected after 6 h. 18,19…”

Section: Animal Protocol and Experimental Designmentioning

confidence: 99%

Section: Detection Of Lung Wet-to-dry Weight Ratiomentioning

confidence: 99%

Section: Western Blot Assay In Lungsmentioning

confidence: 99%

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