Protective effect of gallic acid and<i>Syzygium cumini</i>extract against oxidative stress-induced cellular injury in human lymphocytes

Bona, Karine Santos De; Bonfanti, Gabriela; Bitencourt, Paula Eliete Rodrigues; Silva, Thainan Paz da; Borges, Raphaela Maleski; Boligon, Aline Augusti; Pigatto, Aline Grohe Schirmer; Athayde, Margareth L.; Moretto, Maria Beatriz

doi:10.3109/01480545.2015.1084631

Cited by 24 publications

(15 citation statements)

References 46 publications

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“…Antioxidant capacity of S. cumini leaf has been formerly shown for hydroethanolic [ 12 ] and aqueous [ 36 ] extracts, whereas PESc apparently present higher capacity. Both quercetin and gallic acid showed similar scavenging capacity of DPPH • compared to the extract, while myricetin presented higher IC 50 .…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of a Polyphenol‐Rich Extract from Syzygium cumini (L.) Skeels Leaf on Oxidative Stress‐Induced Diabetic Rats

Chagas

Coelho

Gaspar

et al. 2018

Oxidative Medicine and Cellular Longevity

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Syzygium cumini (L.) Skeels has been reported to exert anti-inflammatory and cardiometabolic activities due to its high content of polyphenols. We characterized the chemical composition and assessed the antidiabetic effects of a novel polyphenol-rich extract (PESc) obtained from S. cumini leaf. Rats were injected with alloxan (150 mg/kg, ip, ALX group) and followed up for 7 days. Some were orally treated with PESc (50 mg/kg/day) for 7 days before and after diabetes induction (ALX-PP) or only for 7 days after alloxan injection (ALX-P). ALX-P and ALX-PP decreased fasting glycemia in 37 and 43%, respectively, as compared to ALX. Triglycerides and total cholesterol serum levels were also significantly reduced in comparison to ALX. PESc presented high polyphenol concentration (71.78 ± 8.57 GAE/100 g), with flavonoid content of 8.21 ± 0.42 QE/100 g. Upon HPLC-MS/MS and MS/MS studies, five main polyphenols—gallic acid, quercetin, myricetin, and its derivatives—were identified. Myricetin was predominant (192.70 ± 16.50 μg/mg PESc), followed by measurable amounts of gallic acid (11.15 ± 0.90 μg/mg PESc) and quercetin (4.72 ± 0.06 μg/mg PESc). Kinetic assessment of total antioxidant capacity revealed PESc high potency, since maximum response was reached within 5 min reaction time in a concentration-dependent manner. Specific antioxidant activity of PESc was assessed against both DPPH• and ABTS•+, showing strong activity (IC50: 3.88 ± 1.09 and 5.98 ± 1.19 μg/mL, resp.). PESc also inhibited lipoxygenase activity (IC50: 27.63 ± 8.47), confirming its antioxidant activity also on biologically relevant radicals. Finally, PESc induced insulin secretion by directly stimulating INS-1E β cells in the absence of any cytotoxic effect. Overall, our results support that PESc is a potent antioxidant phytocomplex with potential pharmacological use as a preventive antidiabetic natural product.

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Section: Discussionmentioning

confidence: 99%

Protective Effects of a Polyphenol‐Rich Extract from Syzygium cumini (L.) Skeels Leaf on Oxidative Stress‐Induced Diabetic Rats

Chagas

Coelho

Gaspar

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…All the changes can be caused by oxidative stress, such as cancer suppressor [23], cell normal functions [24], special responses for pathogens [25], maximal actions, and antigen presentation [26] (Figure 6). Pathway analysis revealed that, in intestinal mucosa tissues of participants from high altitude, many pathway genes had aberrant expression and may be also related with oxidative stress, especially in inflammatory bowel diseases [27, 28], myeloperoxidase [29], ROS production [10], apoptotic cell death [30], tissue damage [31], interleukin-1 [32], oxidative modification [33], cystic fibrosis [34], natural killer cells activity [35], lymphocytes [36], fibrinolysis [37] and so on (Figure 7). …”

Section: Resultsmentioning

confidence: 99%

Genome‐Wide Transcriptional Analysis Reveals the Protection against Hypoxia‐Induced Oxidative Injury in the Intestine of Tibetans via the Inhibition of GRB2/EGFR/PTPN11 Pathways

Gesang

Zhang

et al. 2016

Oxidative Medicine and Cellular Longevity

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The molecular mechanisms for hypoxic environment causing the injury of intestinal mucosal barrier (IMB) are widely unknown. To address the issue, Han Chinese from 100 m altitude and Tibetans from high altitude (more than 3650 m) were recruited. Histological and transcriptome analyses were performed. The results showed intestinal villi were reduced and appeared irregular, and glandular epithelium was destroyed in the IMB of Tibetans when compared with Han Chinese. Transcriptome analysis revealed 2573 genes with altered expression. The levels of 1137 genes increased and 1436 genes decreased in Tibetans when compared with Han Chinese. Gene ontology (GO) analysis indicated most immunological responses were reduced in the IMB of Tibetans when compared with Han Chinese. Gene microarray showed that there were 25-, 22-, and 18-fold downregulation for growth factor receptor-bound protein 2 (GRB2), epidermal growth factor receptor (EGFR), and tyrosine-protein phosphatase nonreceptor type 11 (PTPN11) in the IMB of Tibetans when compared with Han Chinese. The downregulation of EGFR, GRB2, and PTPN11 will reduce the production of reactive oxygen species and protect against oxidative stress-induced injury for intestine. Thus, the transcriptome analysis showed the protecting functions of IMB patients against hypoxia-induced oxidative injury in the intestine of Tibetans via affecting GRB2/EGFR/PTPN11 pathways.

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“…The diversity and complexity of ellagic acid derivatives is actually determined on the type and amount of functional groups which can replace the hydroxyl groups either by methoxyl groups and the glycosyl groups mostly at position C-3, C-4, C-3' and C-4'. From here, for instance 3,3'-di-O-methylellagic acid (Reynertson, 2007) Simoes-Pires et al, 2009) has been isolated from S. cumini, beside ellagic acid its self (Simoes-Pires et al, 2009;De Bona et al, 2016). (Djoukeng, Mansour, Tapondjou, Lontsi & Tabacchi, 2007;Khan, Khan, Sahreen, & Ahmed, 2012).…”

Section: Resultsmentioning

confidence: 99%

Two Phenolic Compounds from Chloroform Fraction of Syzygium Polycephalum MIQ. Stem Bark (Myrtaceae)

Tukiran

Wardhana

Hidajati

et al. 2018

Molekul

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Syzygium polycephalum (Kupa) is a plant of the Myrtaceae family which is one of the endemic plants in Indonesia, commonly called as Gowok. The chemical components of the plant have not been reported so far. This study is intended to know the molecular structures of isolated compounds of chloroform fraction from S. polycephalum stem bark. The steam bark of the plant is dried, powdered and macerated with methanol to yield methanolic extract. The methanolic extract was then conducted to fractionation using hexane and chloroform to obtain hexane and chloroform fractions. The chloroform fraction was further subjected to separation using column chromatography to obtain pure isolates and followed by measuring of their spectroscopic evidences. The isolation of chloroform fraction had led to the findings of two pure isolates. Their structures of isolates were elucidated by extensive spectroscopic methods and by comparison with the literature data to gain two phenolic compounds that are gallic acid and 3,4,3'-tri-O-methylellagic acid.

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Protective effect of gallic acid andSyzygium cuminiextract against oxidative stress-induced cellular injury in human lymphocytes

Abstract: ASc affects the purinergic system and may modulate adenosine levels, indicating that the extract of this plant exhibits immunomodulatory properties. ASc also may potentially prevent the cellular injury induced by oxidative stress, highlighting its cytoprotective effects.

Cited by 24 publications

References 46 publications

Protective Effects of a Polyphenol‐Rich Extract from Syzygium cumini (L.) Skeels Leaf on Oxidative Stress‐Induced Diabetic Rats

Protective Effects of a Polyphenol‐Rich Extract from Syzygium cumini (L.) Skeels Leaf on Oxidative Stress‐Induced Diabetic Rats

Genome‐Wide Transcriptional Analysis Reveals the Protection against Hypoxia‐Induced Oxidative Injury in the Intestine of Tibetans via the Inhibition of GRB2/EGFR/PTPN11 Pathways

Two Phenolic Compounds from Chloroform Fraction of Syzygium Polycephalum MIQ. Stem Bark (Myrtaceae)

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