Protective effect of Fructose-1,6-Bisphosphate in the cold storage solution for liver preservation in rat hepatic transplantation

Moresco, Rafael Noal; Santos, Roberto Christ Vianna; Filho, José Carlos Farias Alves; Cunha, Aline Andrea da; Reis, Carolina dos; Reichel, Carlos Luiz; Oliveira, Jarbas Rodrigues de

doi:10.1016/j.transproceed.2004.05.040

Cited by 15 publications

(12 citation statements)

References 13 publications

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“…A variety of ingredients such as the stable prostacyclin (PGI2) analogue OP-4183 (Goto et al, 1993), the p38 mitogen-activated protein kinase (MAPK) inhibitor FR167653 (Yoshinari et al, 2001), the NO donor sodium nitroprusside (Rodriguez et al, 1999), the platelet-activating factor (PAF) antagonist E5880 (Takada et al, 1995), calmodulin inhibitors (Anaise et al, 1990), Ca 2+ channel blockers such as nisoldipine (Takei et al, 1990;Vajdova et al, 2002), trophic factors (Ambiru et al, 2004), caspase or calpain inhibitors, S-adenosylmethionine (SAM) (Vajdova et al, 2002), insulin (Li et al, 2003), or fructose-1,6-biphosphate (FBP) (Moresco et al, 2004) were introduced into UW preservation solution, with promising results. However, none of these modifications to UW solution composition have found their way into routine clinical practice.…”

Section: Preservation Solutionsmentioning

confidence: 99%

Past and future approaches to ischemia-reperfusion lesion associated with liver transplantation

et al. 2006

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Section: Preservation Solutionsmentioning

confidence: 99%

Past and future approaches to ischemia-reperfusion lesion associated with liver transplantation

et al. 2006

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“…Indeed, that extended time is not often used, since more than 12 hours carries high risk of injury, mainly of biliary cells (1,3) . Moreover, in a previous study, 12 hours of cold preservation were enough to demonstrate any injury (11) . In this context, defining old rats seems essential.…”

Section: Discussionmentioning

confidence: 80%

“…It seems that FBP could exert some protective effects on grafts from older donors. Moresco et al (11) had demonstrated that FBP is able to reduce COI injury when compared to UW solution in normal fed young rats. So, our results were quite similar with their.…”

Section: Discussionmentioning

confidence: 99%

“…In this way, fructose-1,6-bisphosphate (FBP) may have some utility. It is an energetic mediator of glycolytic route, which has been studied in the field of cell protection in many pathological situations, as sepsis, and ischemia/reperfusion in organs other than the liver (11) . The aim of this study was to determinate the impact of the aging of the donor on the cold ischemia injury (COI) in rat livers, as well to compare two distinct solutions of preservation: UW and FBP.…”

Section: Introductionmentioning

confidence: 99%

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Aging and its impact onthe quality of grafts: an experimental study in rats livers

Mota¹,

Gasperin²,

Cerski³

et al. 2010

Arq. Gastroenterol.

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-Context -There are consistent clues of worse results with grafts from older donors, especially in hepatitis C. University of Wisconsin (UW) solution is adopted for liver preservation, but other solutions are being studied, as fructose-1,6-bisphosphate (FBP). Objective -To determinate the impact of aging of the donor on the cold ischemia injury in rat livers and compare UW and FBP. Methods -Twenty male Wistar rats were studied -10, aging 20 weeks: 5 to preservation with UW (C-UW) and 5, FBP (C-FBP); and other 10, aging 50 weeks: 5 to UW (E-UW) and 5 to FBP (E-FBP). Rats were anesthesized, submitted to hepatectomy, and graft was kept under 2-4ºC for 8 hours. Liquid samples were taken at 2, 4, 6, and 8 hours, to determine AST and LDH. At the end, in the liver tissue thiobarbituric acid reactive substances and catalase were determined. Results -Average liver weight was similar between controls and the others (12.5 g ± 1.8 and 13.4 g ± 2.0). AST and LDH levels were higher in C-UW group (P<0.05). In the older group, there was a difference between UW and FBP preserved livers related to LDH, but not to AST. Thiobarbituric acid reactive substances were superior in control group than in the older one (P = 0.001). Catalase activity was similar between these groups (P = 0.11), but it was superior in UW preserved animals (P = 0.02). Conclusion -Livers from older rats were similar to the controls regarding cold ischemia injury in FBP group. Surprisingly, with UW solution there was less cold ischemia injury in the elderly group. When comparing both solutions, FBP provided significantly more protection than UW in the controls. There was a trend to FBP to being better than UW in the elderly group. Further studies with liver from older donors and ischemia and reperfusion are needed.

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“…FBP has been used as a component of protective solutions in the transport of donor organs, such as liver (84). Other investigators have filed a patent on FBP as a therapeutic for asthma (85), and a recent in vivo study in rats demonstrated that FBP has an inhibitory role on MC degranulation and histamine release (86).…”

mentioning

confidence: 99%

Protein Tyrosine Nitration of Aldolase in Mast Cells: A Plausible Pathway in Nitric Oxide-Mediated Regulation of Mast Cell Function

Sekar

Moon

Slupsky

et al. 2010

The Journal of Immunology

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Protective effect of Fructose-1,6-Bisphosphate in the cold storage solution for liver preservation in rat hepatic transplantation

Cited by 15 publications

References 13 publications

Past and future approaches to ischemia-reperfusion lesion associated with liver transplantation

Past and future approaches to ischemia-reperfusion lesion associated with liver transplantation

Aging and its impact onthe quality of grafts: an experimental study in rats livers

Protein Tyrosine Nitration of Aldolase in Mast Cells: A Plausible Pathway in Nitric Oxide-Mediated Regulation of Mast Cell Function

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