Protective Effect of Ethanolic Extract of Seeds ofMoringa oleiferaLam. Against Inflammation Associated with Development of Arthritis in Rats

Abstract

The present investigation was carried out to study the anti-arthritic activity of ethanolic extract of seeds of Moringa oleifera Lam. (MOEE) in adjuvant-induced arthritis in adult female Wistar rats. During the experimental period, body weight, paw edema volume (primary lesion) and arthritic index (secondary lesion) was observed. On the 21st day, serum from each animal was used for estimation of Rheumatoid Factor (RF) value and levels of selected cytokines (TNFalpha, IL-1, and IL-6). Whole blood was used for measurement of erythrocyte sedimentation rate (ESR). Liver homogenate was utilized for assessment of oxidative stress and histopathology was performed to measure degree of inflammation in synovial joint. Our results suggest that, percentage reduction in body weight was less, paw edema volume and arthritic index score was decreased significantly as compared to diseased control animals. Serum levels of RF, TNF-alpha, IL-1, and IL-6 also showed decreased levels as compared to those in the diseased control group. Treatment with MOEE also altered oxidative stress in relation to its anti-inflammatory activity. Histopathological observations showed mild or less infiltration of lymphocytes, angiogenesis and synovial lining thickening. From all above results and observations, it can be concluded that Moringa oleifera possesses promising antiarthritic property.

Role of <i>Bifidobacterium bifidum</i> and plant food extracts in improving microflora and biochemical and cytogenetic parameters in adjuvant arthritis.

“…The assessed inflammatory biomarkers, ESR and TNF-α, showed extreme elevation in the control adjuvant arthritic rats compared to the normal control. This result was in agreement with the work of Glenn & Kooyers (1966) and Mahajan et al (2007).…”

Evaluation of the anti-inflammatory and anti-arthritic effects of some plant extracts

“…TNF-α was the biomarker of inflammation assessed in the present study and showed extreme elevation in the control adjuvant arthritic rats compared to the normal control. This result was in agreement with the work of Glenn & Kooyers (1966) and Mahajan et al (2007). The elevation in the plasma levels of MDA in the arthritic control rats in the current research is in In each column the same letter means non significant difference, while different letters mean significant difference at 0.05 probability.…”

Protective Effects of Moringa oleifera extract on Isoniazid and Rifampicin Induced Hepatotoxicity in Rats: Involvment of Adiponectin and Tumor Necrosis Factor-α

“…Our study agrees with Mahmoud [57]. The hepatoprotective effect of Moringa oleifera against liver inflammation is attained by its reduction of the liver content of TNF-α and this was agreed with Mahajan et al [58]. The same action also happened with administration of silymarin that decreased TNF-α liver content, these results agree with Song et al [59].…”