Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species

Kim, So-hyung; Kang, Kyoung-Ah; Zhang, Rui; Piao, Meijing; Ko, Dong-ok; Wang, Zhihong; Chae, Sungwook; Kang, Sam-Sik; Lee, Keun-hwa; Kang, Hee‐Kyoung; Kang, Hyun‐Wook; Hyun, Jin‐Won

doi:10.1111/j.1745-7254.2008.00878.x

Cited by 99 publications

(60 citation statements)

References 32 publications

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“…Esculetin is recognized as an inhibitor not only of the lipoxygenase and cyclooxygenase enzymatic systems but also for the neutrophil-dependent superoxide anion generation system (Fylaktakidou et al 2004). It has been shown to exhibit 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, hydroxyl radical scavenging, and intracellular ROS scavenging activities (Kim et al 2008). The radical scavenging activity of esculetin resulted in the protection of cells from lipid peroxidation, protein carbonyl, and DNA damage induced by H 2 O 2 .…”

Section: Resultsmentioning

confidence: 99%

Esculetin induced changes in Mmp13 and Bmp6 gene expression and histone H3 modifications attenuate development of glomerulosclerosis in diabetic rats

Surse¹,

Gupta²,

Tikoo³

2011

Journal of Molecular Endocrinology

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Esculetin, an antioxidant, has been used in the treatment of a variety of diseases. This study aimed to investigate the protective effect of esculetin in attenuating streptozotocin (STZ)-induced type I diabetic nephropathy and to understand the molecular mechanism involved in it. Sprague-Dawley rats were rendered diabetic using a single dose of STZ (55 mg/kg, i.p.). Protein expression of PPARg and transforming growth factor-b1 (TGF-b1) was detected by immunoblotting and immunohistochemistry respectively. RNA expression levels of Mmp13 and Bmp6 were detected by RT-PCR analysis. In diabetic rats, esculetin treatment resulted in a significant decrease in blood glucose, blood urea nitrogen, and plasma creatinine and increase in plasma albumin levels. Esculetin treatment attenuates the downregulation of PPARg in diabetic kidney, which in turn blocks the TGF-b1-mediated fibronectin expression. In addition, it attenuates the decrease in mono-methylation (K4) and acetylation of histone H3 in diabetic kidney. RT-PCR analysis revealed that esculetin treatment provides protection by decreasing antifibrotic Bmp6 and increasing fibrogenic Mmp13 mRNA expression in diabetic kidney. This is the first report to show that protection observed by esculetin treatment involves alteration in mRNA expression of Mmp13 and Bmp6 genes either directly via altered histone H3 modifications or indirectly by inhibiting the PPARg/TGF-b1 pathway.

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Section: Resultsmentioning

confidence: 99%

Esculetin induced changes in Mmp13 and Bmp6 gene expression and histone H3 modifications attenuate development of glomerulosclerosis in diabetic rats

Surse¹,

Gupta²,

Tikoo³

2011

Journal of Molecular Endocrinology

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“…Lipid peroxidation was assayed by the measurement of related substances that react with thiobarbituric acid (TBARS) [43]. The V79-4 cells were seeded in a culture dish at a concentration of 1 × 105 cells/mL and treated with esculetin at 10 g/mL after 16 h plating and then hydrogen peroxide (H 2 O 2 ) 1 mmol/L was added to the plate after 1 h, which was incubated for a further 24 h. The cells were then washed with cold PBS, scraped, and homogenized in ice-cold 1.15% potassium chloride (KCl).…”

Section: Lipid Peroxidation Detectionmentioning

confidence: 99%

Human mesenchymal stem cells provide protection against radiation-induced liver injury by antioxidative process, vasculature protection, hepatocyte differentiation and trophic effects

François¹,

Mouiseddine²,

Allenet-Lepage³

et al. 2014

Cytotherapy

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To evaluate the potential therapeutic effect of the infusion of hMSCs for the correction of liver injuries, we performed total body radiation exposure of NOD/SCID mice. After irradiation, mir-27b level decreases in liver, increasing the directional migration of hMSCs by upregulating SDF1 . A significant increase in plasmatic transaminases levels, apoptosis process in the liver vascular system, and in oxidative stress were observed. hMSC injection induced a decrease in transaminases levels and oxidative stress, a disappearance of apoptotic cells, and an increase in Nrf2, SOD gene expression, which might reduce ROS production in the injured liver. Engrafted hMSCs expressed cytokeratin CK18 and CK19 and AFP genes indicating possible hepatocyte differentiation. The presence of hMSCs expressing VEGF and Ang-1 in the perivascular region, associated with an increased expression of VEGFr1, r2 in the liver, can confer a role of secreting cells to hMSCs in order to maintain the endothelial function. To explain the benefits to the liver of hMSC engraftment, we find that hMSCs secreted NGF, HGF, and anti-inflammatory molecules IL-10, IL1-RA contributing to prevention of apoptosis, increasing cell proliferation in the liver which might correct liver dysfunction. MSCs are potent candidates to repair and protect healthy tissues against radiation damages.

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“…Assessment of intracellular ROS production ROS production was quantified by the DCFH-DA method [20] , based on the ROS-dependent oxidation of DCFH-DA to DCF. VSMCs grown in 96-well plates were treated with different concentrations of LA for 2 h prior to LPS (100 ng/mL) or FBS (5%) stimulation for 30 min at 37 °C.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory effects of lithospermic acid on proliferation and migration of rat vascular smooth muscle cells

Chen

Wang

2009

Acta Pharmacol Sin

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Aim: To understand the effects of lithospermic acid (LA), a potent antioxidant from the water-soluble extract of Salvia miltiorrhiza, on the migration and proliferation of rat thoracic aorta vascular smooth muscle cells (VSMCs). Methods: VSMC migration, proliferation, DNA synthesis and cell cycle progression were investigated by transwell migration analysis, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, bromodeoxyuridine (BrdU) incorporation assay, and flow cytometric detection, respectively. Intracellular reactive oxygen species (ROS) generation was detected using 2′,7′-dichlorofluorescin diacetate (DCFH-DA). The expression of cyclin D1 protein and matrix metalloproteinase-9 (MMP-9) protein, as well as the phosphorylation state of ERK1/2, were determined using Western blots. The activity of MMP-9 and the expression of MMP-9 mRNA were assessed by gelatin zymography analysis and RT-PCR, respectively. Results: LA (25−100 μmol/L) inhibited both lipopolysaccharide (LPS)-and fetal bovine serum (FBS)-induced ROS generation and ERK1/2 phosphorylation. By down-regulating the expression of cyclin D 1 and arresting cell cycle progression at the G 1 phase, LA inhibited both VSMC proliferation and DNA synthesis as induced by 5% FBS. Furthermore, LA attenuated LPS-induced VSMC migration by inhibiting MMP-9 expression and its enzymatic activity. Conclusion: LA is able to inhibit FBS-induced VSMC proliferation and LPS-induced VSMC migration, which suggests that LA may have therapeutic effects in the prevention of atherosclerosis, restenosis and neointimal hyperplasia.

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Protective effect of esculetin against oxidative stress-induced cell damage via scavenging reactive oxygen species

Cited by 99 publications

References 32 publications

Esculetin induced changes in Mmp13 and Bmp6 gene expression and histone H3 modifications attenuate development of glomerulosclerosis in diabetic rats

Esculetin induced changes in Mmp13 and Bmp6 gene expression and histone H3 modifications attenuate development of glomerulosclerosis in diabetic rats

Human mesenchymal stem cells provide protection against radiation-induced liver injury by antioxidative process, vasculature protection, hepatocyte differentiation and trophic effects

Inhibitory effects of lithospermic acid on proliferation and migration of rat vascular smooth muscle cells

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