Protective Effect of (-)-Epigallocatechin Gallate against Photo-Damage Induced by Ultraviolet A in Human Skin Fibroblasts

Shin, Sae-Ron; Lx, Wang; Zheng, Xia; Xiang, L-P; Liang, YR

doi:10.4314/tjpr.v13i7.10

Cited by 7 publications

(8 citation statements)

References 27 publications

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“…Cytoprotective effects of GSE on human gingival fibroblasts from several oxidative stressors were previously reported and thought to be independent of its direct antioxidant action 24 . Contrary to these findings, it was shown that major catechin of green tea, epigallocatechin gallate (EGCG), improved the viability of dermal fibroblasts exposed to UVA along with the inhibition of UV‐induced generation of oxidative stressors including superoxide anion radicals, lipid peroxidation, and malondialdehyde (MDA) and suppression of glutathione peroxidase (GSH‐Px) activity 25 . In addition, the topical treatment of human epidermal keratinocytes with grape seed proanthocyanidins (GSPs) was able to inhibit lipid and protein oxidation, and support the skin's own antioxidant system associated with the reduction in UV‐induced oxidative damage 26 .…”

Section: Discussionmentioning

confidence: 98%

Effect of grape seed extract on skin fibroblasts exposed to UVA light and its photostability in sunscreen formulation

Yarovaya

Waranuch

Wisuitiprot

et al. 2020

J of Cosmetic Dermatology

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Background Grape seed extract (GSE) is rich in polyphenolic compounds, particularly (+)‐catechin (C) and (‐)‐epicatechin (EC). Strong antioxidant activity of these compounds makes GSE to be value‐added to the cosmetics with anti‐aging properties. However, a lack of stability in different environmental conditions makes GSE challenging for the development of photostable cosmetic sunscreen products. Aims To evaluate photoprotective effects of GSE on human dermal fibroblasts irradiated with UVA light and assess photostability of catechins in cream formulations containing GSE alone or in combination with octyl methoxycinnamate (OMC). Methods MTT assay was used to assess protective effects of GSE on fibroblasts irradiated with UVA light. A photostability of C and EC in GSE and in cream formulation containing GSE was investigated using high‐performance liquid chromatography and confirmed by reflection and transmission spectrophotometry using Transpore™ tapes and polymethacrylate (PMMA) plates as substrates. Results High UVA doses damaged fibroblast structure and inhibited their growth. However, GSE increased cell viability and effectively protected them from UVA damage. Photostability of C and EC was achieved by combination of GSE and OMC that also improved absorption capacity of UV filter and increased overall efficacy of formulation. PMMA plates showed better applicability for in vitro photostability testing of sunscreen formulations. However, despite the instability of Transpore® tape under heat from UV exposure, it can still be economically a substrate of alternative choice for screening. Conclusions GSE can be used as an effective and sustainable natural resource for prevention of UV‐induced skin damage providing long‐term protection against premature skin aging.

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Section: Discussionmentioning

confidence: 98%

Effect of grape seed extract on skin fibroblasts exposed to UVA light and its photostability in sunscreen formulation

Yarovaya

Waranuch

Wisuitiprot

et al. 2020

J of Cosmetic Dermatology

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“…Inulavosin might compete with the copper chaperon and inhibit the copper loading processing in living melanocytes [15]. Green tea EGCG is a benzoderivative with 8 hydroxyl groups, which has strong antioxidant activity [8,16]. It is deduced that the melanogenesis inhibitory effect of EGCG may be related to its abundant hydroxyl groups and its antioxidant activity.…”

Section: Discussionmentioning

confidence: 98%

“…EGCG protects human skin fibroblasts from photo-damage induced by UVA [8] and reduces melanin synthesis via reduction of the protein levels of MITF [9,10]. The present study set to investigate the effect of EGCG on TRY activity and melanogenesis in UV A induced B16 murine melanoma cell lines.…”

Section: Introductionmentioning

confidence: 95%

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Inhibitory effects of (-)-epigallocatechin-3-gallate on melanogenesis in ultraviolet A-induced B16 murine melanoma cell

Liang

Kang²,

Deng³

et al. 2014

Trop. J. Pharm Res

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Purpose: To investigate the anti-melanogenesis effect of green tea compound, (-)-epigallocatechin-3gallate (EGCG), on B16 murine melanoma cell irradiated by ultraviolet A (UVA) in the search for natural skin-lightening alternative agents.Methods: B16 murine melanoma cells by UVA (9.0 J/cm 2 ) for 0 to 32 min and then incubated in Dulbecco's Modified Eagle's Medium (DMEM) with EGCG (0-200 μg/mL) for 2 days. Cell viability was determined by MTT method and cell protein was quantified using a PA102 Bradford protein assay kit. Activity of tyrosinase (TRY) was determined based on the oxidation rate of 3,4-dihydroxy phenylalanine (DOPA). The ultra-structure of the melanosomes was observed by transmission electron microscopy (TEM). Results: TRY activity and melanin concentration were increased to 146.70 ± 10.28 % (p < 0.05) and 157.06 ± 6.37 % (p < 0.05), respectively, by 9.0 J/cm2 UVA irradiation for 8 min, compared to blank control without UV A and EGCG. EGCG inhibited the UV A induced increase in TRY activity and melanin level, and the optimum concentration of EGCG was 25 μg/mL. TRY activity and melanin concentration were decreased to 64.71 ± 4.41 (p < 0.05) and 86.24 ± 5.15 % (p < 0.05), respectively, compared to blank (control) which was neither treated by UVA nor by EGCG. TEM showed that UVA induced the formation of melanosomes while EGCG inhibited UVA-induced melanosome maturation. Conclusion: EGCG inhibits UVA-induced melanogenesis via suppression of TRY activity and melanosome maturation and is thus a potential alternative to melanogenesis inhibitor.

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“…Perubahan radikal peroksil melalui reaksi siklisasi menjadi endoperoksida yang pada akhirnya akan menghasilkan MDA. 31 Senyawa MDA merupakan produk peroksidasi lipid yang paling mutagenik dan digunakan sebagai biomarker peroksidasi lipid asam lemak omega-3 serta omega-6 karena kemudahannya bereaksi dengan thiobarbituric acid (TBA). 32 Akumulasi MDA di dalam sel dapat mengakibatkan terjadinya degradasi seluler, perubahan biokimia dan fungsional, bahkan kematian sel.…”

Section: Spirulina Platensisunclassified

Potency of Spirulina platensis Extract as Sunscreen on Ultraviolet B Exposure

Pratama¹,

Hartawan²,

Indriani³

et al. 2020

JMH

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Radiasi sinar matahari berupa sinar ultraviolet (UV) dapat menembus lapisan ozon dan berpenetrasi ke dalam lapisan kulit. Dampak paparan sinar UVB jangka panjang adalah pigmentasi kulit, penuaan dini, kulit terbakar, dan juga kanker. Pemanfaatan tabir surya alami sedang diteliti lebih lanjut untuk mengurangi efek samping tabir surya dengan bahan kimia. Tujuan penulisan artikel ini adalah untuk menelaah lebih lanjut potensi ekstrak Spirulina platensis sebagai tabir surya alami terhadap paparan UVB. Ekstrak Spirulina platensis mengandung antioksidan fikosianin dan fikosianobilin yang berpotensi sebagai tabir surya. Ekstrak Spirulina platensis mampu menurunkan kadar malondialdehid (MDA) setelah paparan sinar UVB, sehingga menekan degradasi seluler, perubahan biokimia dan fungsional, serta menurunkan terjadinya apoptosis sel. Efek lain setelah pemaparan UVB pada sel kulit dengan ekstrak Spirulina platensis adalah terjadi peningkatan viabilitas fibroblast dan deposisi kolagen, yakni dengan menekan terbentuknya MMP serta kandungan tinggi vitamin C dalam ekstrak berperan sebagai konversi prokolagen menjadi kolagen. Berdasarkan hal tersebut, disimpulkan bahwa ekstrak Spirulina platensis memiliki potensi sebagai agen protektif terhadap paparan sinar UVB. Kata kunci: Ultraviolet B (UVB); Fibroblas; Spirulina platensis; Antioksidan

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Protective Effect of (-)-Epigallocatechin Gallate against Photo-Damage Induced by Ultraviolet A in Human Skin Fibroblasts

Cited by 7 publications

References 27 publications

Effect of grape seed extract on skin fibroblasts exposed to UVA light and its photostability in sunscreen formulation

Effect of grape seed extract on skin fibroblasts exposed to UVA light and its photostability in sunscreen formulation

Inhibitory effects of (-)-epigallocatechin-3-gallate on melanogenesis in ultraviolet A-induced B16 murine melanoma cell

Potency of Spirulina platensis Extract as Sunscreen on Ultraviolet B Exposure

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