Protective Effect of Epigallocatechin-3-Gallate in Hydrogen Peroxide-Induced Oxidative Damage in Chicken Lymphocytes

Abstract

Epigallocatechin-3-gallate (EGCG) is one of the fundamental compounds in green tea. The present study was to evaluate the protective effect of EGCG in oxidative damage and apoptosis induced by hydrogen peroxide (H2O2) in chicken lymphocytes. Results showed that preincubation of lymphocytes with EGCG significantly decreased H2O2-reduced cell viability and apoptotic cells with DNA damage, restored the H2O2-dependent reduction in total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-PX), superoxide dismutase (SOD), glutathione (GSH), and glutathione disulfide (GSSG), and suppressed the increase in intracellular reactive oxygen species (ROS), nitric oxide (NO), nitric oxide synthesis (NOS), malondialdehyde (MDA), lipid peroxide (LPO), and protein carbonyl (Carbonyl). In addition, preincubation of the cells with EGCG increased mitochondrial membrane potential (MMP) and reduced calcium ion ([Ca2+]i) load. The protective effect of EGCG in oxidative damage in lymphocytes was accompanied by mRNA expression of SOD, Heme oxygenase-1 (HO-1), Catalase (CAT), GSH-PX, nuclear factor erythroid 2-related factor 2 (Nrf2), and thioredoxin-1 (Trx-1). As EGCG had been removed before lymphocytes were challenged with H2O2, the activation of genes such as Nrf2 and Trx-1 by preincubation with EGCG could be the main reason for EGCG to protect the cells from oxidative damage by H2O2. Since oxidative stress is an important mechanism of biological damage and is regarded as the reasons of several pathologies, the present findings may be helpful for the use of tea products to prevent oxidative stress and maintain healthy in both humans and animals.

Effect of dietary BHA supplementation on certain physiological values in broiler chicken

“…The results also noted a significant upraise in the level of thyroid hormones, and no study was recorded on the effect of BHA on the level of these hormones. However, it is believed that its role in this increase can be attributed to improving the health of the bird through increasing the level of SOD and CAT, which is one of the first lines of defense in the body (19), where superoxide dismutase works to break down free radicals and convert them to hydrogen peroxide. In contrast, catalase works to convert hydrogen peroxide into water and oxygen (20).…”

Pretreatment of nucleus pulposus mesenchymal stem cells with appropriate concentration of H2O2 enhances their ability to treat intervertebral disc degeneration

“…Previous studies have shown that pretreatment with an appropriate concentration of H 2 O 2 can significantly improve the proliferation, paracrine manner, antiapoptotic ability, and therapeutic effect of MSCs in liver fibrosis, skin wounds, and myocardial infarction [ 24 – 26 ]. However, a high concentration of H 2 O 2 is cytotoxic and will cause an increase in ROS, which may lead to irreversible oxidative damage to the cells [ 27 , 28 ].…”

Pretreatment of NPMSCs with Appropriate Concentration of H2O2 Enhances its Ability to Treat IVDD

“…Previous studies have shown that pretreatment with an appropriate concentration of H 2 O 2 can signi cantly improve the proliferation, paracrine manner, antiapoptotic ability, and therapeutic effect of MSCs in liver brosis, skin wound, and myocardial infarction [24][25][26]. However, a high concentration of H 2 O 2 is cytotoxic and will cause the increase of reactive oxygen species (ROS), which may lead to irreversible oxidative damage to the cells [27,28]. Therefore, exploring the optimal concentration of H 2 O 2 pretreatment is of great signi cance for improving the effect of NPMSCs in the treatment of IVDD.…”

“…. A high concentration of H 2 O 2 can induce a surge of intracellular ROS, which damages the organelles and results in cell cycle arrest and cell apoptosis[27]. However, pretreatment of transplanted cells with H 2 O 2 at appropriate concentrations can result in bene cial effects.…”

“…In comparison, 300 μM H 2 O 2 treatment signi cantly inhibited the expression of cyclin D1 and signi cantly raised the expression of P16 (Fig.2d, 2e). These results showed that 75 μM H 2 O 2 could promote the proliferation of NPMSCs.The Hippo pathway is a crucial signaling pathway that can regulate cell proliferation and cell growth[27]. We speculated that H 2 O 2 may regulate the proliferation of NPMSCs by inhibiting the Hippo pathway.…”