Protective effect of delta opioid agonist [d-Ala2, d-Leu5] enkephalin on spinal cord ischemia reperfusion injury by regional perfusion into abdominal aorta in rabbits

Liu, Haitong; Chen, Binbin; Zhang, Yi; Qiu, Yimin; Xia, Yunfei; Li, Shitong; Yao, Junyan

doi:10.1016/j.neulet.2014.09.048

Cited by 14 publications

(8 citation statements)

References 26 publications

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“…Thus, in addition to the analgesic, anxiolytic, d-Opioid Receptor Pharmacology and antidepressant actions (Ribeiro et al, 2005;Hsu et al, 2015) that were mentioned in previous sections, opioids also promote cell survival (Hayashi et al, 2002;Ma et al, 2005), cardioprotection (Ikeda et al, 2006;Tsutsumi et al, 2010;Headrick et al, 2015), neuroprotection (He et al, 2013b;Liu et al, 2015), modulation of the immune/inflammatory response (Neptune and Bourne, 1997;Hedin et al, 1999;Sharp, 2006;Wang et al, 2014), and wound healing (Bigliardi-Qi et al, 2006;Iaizzo et al, 2012;Bigliardi et al, 2015). At the cellular level, a majority of these responses are mediated by an evolutionarily conserved set of kinase cascades whose activation has been traditionally associated with receptor tyrosine kinases (RTKs) (Takeda et al, 2011).…”

Section: B D-opioid Receptors and Mitogen-activated Protein Kinase Smentioning

confidence: 95%

Molecular Pharmacology ofδ-Opioid Receptors

et al. 2016

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Section: B D-opioid Receptors and Mitogen-activated Protein Kinase Smentioning

confidence: 95%

Molecular Pharmacology ofδ-Opioid Receptors

et al. 2016

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“…Moreover, recent studies such as that of Goes et al . (2014) [ 87 ] and Ravenelle et al . (2014) [ 88 ] have shown that EE is able to reduce the levels of trait anxiety in adult rats, understood as those individual characteristics relatively stable in time, which make the subject more vulnerable to problems of anxiety [ 89 ].…”

Section: What Is the Environmental Enrichmentparadigm?mentioning

confidence: 99%

Environmental Enrichment as a Positive Behavioral Intervention Across the Lifespan

Sampedro-Piquero

Begega

2017

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Background:In recent decades, the interest in behavioral interventions has been growing due to the higher prevalence of age-related cognitive impairments. Hence, behavioral interventions, such as cognitive stimulation and physical activity, and along with these, our lifestyle (education level, work position, frequency of cognitive and social activities) have shown important benefits during the cognitive impairment, dementia and even recovery after brain injury. This is due to the fact that this type of intervention and activities promote the formation of a cognitive and brain reserve that allows tolerating brain damage during a long period of time without the appearance of cognitive symptoms. With regard to this, animal models have proved very useful in providing information about the brain mechanisms involved in the development of these cognitive and brain reserves and how they interact with each other.Methods:We summarize several studies showing the positive effects of Environmental Enrichment (EE), understood as a housing condition in which animals benefit from the sensory, physical, cognitive and social stimulation provided, on brain and cognitive functions usually impaired during aging.Results:Most of studies have shown that EE is a successful protocol to improve cognitive functions and reduce anxiety-related behaviors across the lifespan, as well as in animal models of neurodegenerative diseases.Conclusion:Therefore, EE is a laboratory condition in which some aspects of an active lifestyle are reproduced.

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“…Despite some efforts to develop neuroprotective agents in experimental spinal cord ischemia, few so far has been translated into clinical application. In our previous research, we revealed that the aorta-infused [D-Ala 2 , D-Leu 5 ] enkephalin (DADLE), a synthetic delta-opioid peptide, could approximately reduce the rate of paraplegia by more than 50% and increase the surviving neurons number by 3 times in a rabbit model of spinal cord I/R (Liu et al, 2015a ). In order to achieve more reasonable utilization of agent, it is essential to investigate the mechanisms underlying neuroprotective effects of DADLE in the spinal cord.…”

Section: Introductionmentioning

confidence: 99%

Antioxidative and Antiapoptotic Effects of Delta-Opioid Peptide [D-Ala2, D-Leu5] Enkephalin on Spinal Cord Ischemia-Reperfusion Injury in Rabbits

Liu

et al. 2017

Front. Neurosci.

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Background: In our previous study, we found that regional administration of delta-opioid peptide [D-Ala2, D-Leu5] enkephalin (DADLE) could provide dose-dependent protection on spinal cord ischemia-reperfusion (I/R) injury in rabbits. However, the relative protective molecular mechanisms underlying this neuroprotection remain unclear. The purpose of this study was to investigate whether DADLE provided the protection in spinal cord I/R injury through its antioxidant property by decreasing malondialdehyde (MDA) and nitric oxide (NO) levels and increasing glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels and through its antiapoptotic capacity by inhibiting caspase-3 and p53 expression.Methods: The rabbits were divided into three groups. The animals in Group NS and Group DADLE were administered with normal saline (NS) or DADLE via aorta during 30 min of ischemia respectively, while the one in Group Sham received no intervention. During the period of reperfusion, the rabbit's blood samples were collected for enzyme-linked immunoabsorbent assay (ELISA) examinations of MDA, NO, GSH-Px and SOD. At 48 h after reperfusion, the lumbar spinal cords were harvested for immunohistochemical, real-time polymerase chain reaction (PCR) and western blot studies to detect the caspase-3 and p53 expressions.Results: The activities of serum MDA and NO showed significant reductions in the DADLE group as compared with the control group. By contrast, the levels of serum GSH-Px and SOD were significantly higher in the DADLE group than those in the NS group. In addition, caspase-3 and p53 expression were significantly increased in the NS group, while DADLE mitigated these changes.Conclusions: The protective effects of DADLE at the dosage of 0.05 mg/kg may be related to its antioxidant and antiapoptosis properties in the rabbit model of spinal cord I/R injury.

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Protective effect of delta opioid agonist [d-Ala2, d-Leu5] enkephalin on spinal cord ischemia reperfusion injury by regional perfusion into abdominal aorta in rabbits

Cited by 14 publications

References 26 publications

Molecular Pharmacology ofδ-Opioid Receptors

Molecular Pharmacology ofδ-Opioid Receptors

Environmental Enrichment as a Positive Behavioral Intervention Across the Lifespan

Antioxidative and Antiapoptotic Effects of Delta-Opioid Peptide [D-Ala2, D-Leu5] Enkephalin on Spinal Cord Ischemia-Reperfusion Injury in Rabbits

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