Protective Effect of Chicken Egg Shell Powder Solution (CESP) on Artificially Induced Dental Erosion: An in Vitro Atomic Force Microscope Study

Abstract

Aim: The aim of this in vitro study was to evaluate the effect of chicken egg shell powder solution (CESP) on the dental erosion produced by Coca Cola drink in permanent teeth. Material and methods: Thirty extracted human incisors were collected for this study purpose. After extraction all the specimens were cleaned and sectioned longitudinally resulting in 60 samples from a total of 30 tooth samples. All samples were randomly assigned to the following three groups. Group 1: Untreated group, Group 2: Demineralization by soft cola drink (4 cycles of 2 minutes' immersion at 6 hours' interval), Group 3: Demineralization by soft cola drink (4 cycles of 2 minutes' immersion at 6 hours' interval) followed by 7 days' immersion in CESP. For surface roughness assessment images were taken from AFM. The root means square roughness (Rrms) was calculated from the AFM images. The differences in the mean roughness values between the three groups were analyzed by Anova and Tukey's post hoc test. Results: One way Anova analysis revealed a statistically significant differences with p-value of 0.00, between the mean roughness values of three groups. Tuckey's post hoc test revealed that there is a statistically significant difference (0.158±0.012) between the surface roughness of Group 2 and Group 3 as well as between Group 1 and Group 3(0.085±0.012) with a p-value of 0.00, indicating that following treatment by CESP the mean roughness values decreased significantly. Conclusion: CESP reduces the surface roughness of all treated teeth samples giving encorughment to the protective effect of CESP againts erosive enamel loss.

In-vitro evaluation of remineralization efficiency of chicken eggshell slurry on eroded deciduous enamel

“…Surface roughness and micro-hardness results revealed that, topical treatment with CESP slurry significantly reduced the mean surface roughness and enhanced the micro-hardness of the de-mineralized group II tooth specimens that indicated the process of re-mineralization. The present finding is supported by those of Feroz and Moeen (25) and Feroz et al (26) who recorded enhancement on the hardness and decrease on the surface roughness following the use of CESP. Thus, it was enhancing the in-vitro re-mineralization of eroded enamel.…”

“…Numerous studies tried to evaluate the effects of various re-mineralizing agents on early enamel caries stage, however shortage of data still present about the application of CESP and its efficiency on the treatment of eroded surface of enamel caused by pediatric medications (25,26) .…”

Remineralization Effect of Eggshell Powder on Post-Orthodontic White Spot Lesions Compared to CPP-Acp: A Randomized Controlled Clinical Trial

“…Haghgoo et al (2016) (26) showed that ESP significantly increased the microhardness of artificial enamel carious lesions. Feroz et al (2017) (27) showed that ESP significantly reduced surface roughness of erosive enamel lesions. Mony et al (2015) (28) found that ESP solution can remineralize the enamel, and highlighted the need of studying another suitable vehicle for ESP.…”

“…In addition to the treatment of WSLs, Feroz et al (2017) (27) evaluated the effect of ESP on enamel erosive lesions invitro found that ESP reduced the surface roughness and was effective against erosive enamel lesions. Similarly, Yaberi and Haghgoo (2018) (30) performed an in-situ study to compare the effects of NHA and ESP on the microhardness of third molars enamel following soft drink exposure, and found that both that NHA and ESP have the capability to remineralize erosive lesions.…”

Effect of Chicken Egg Shell Powder and Gallic Acid as Remineralising Agent on Early Enamel Carious LesionAn In Vitro Study

“…In this study atomic analysis by EDX(energy dispersive X-ray spectrometry) was done which shows highest Ca concentration of 98%. According to SandleenFeroz (2017) 11 the protective effects of chicken egg shell powder solution on enamel was compared with samples treated with demineralizing solution and the results showed a statistically significant difference (0.158±0.012) with a p value of 0.00 indicating the effectiveness of remineralizing effect of CESP on enamel surfaces. The statistical results were similar with our results.…”