Protective effect of Cassia fistula Linn. on diethylnitrosamine induced hepatocellular damage and oxidative stress in ethanol pretreated rats

Pradeep, Kannampalli; Chandrasekaran, Victor Raj Mohan; Gobianand, Kuppannan; Karthikeyan, S.

doi:10.4067/s0716-97602010000100013

Cited by 48 publications

(28 citation statements)

References 39 publications

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“…It is not easy to define a profile pattern of antioxidant expression in tumor cells, but it is admissible that the increased number of abnormal cell population may lead to antioxidant gene expression deregulation without any meaning in antioxidant defense. The decreased content of GSH and dependent enzymes -GST and GR -in DEN exposed mice after T2 may support the idea that hepatocellular damage influences antioxidant gene expression; supporting results were also described by Pradeep et al (2010).…”

Section: Discussionsupporting

confidence: 77%

N-diethylnitrosamine mouse hepatotoxicity: Time-related effects on histology and oxidative stress

Santos

Colaço

Costa

et al. 2014

Experimental and Toxicologic Pathology

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Animal models, namely mice, have been used to study chemically induced carcinogenesis due to their similarity to the histological and genetic features of human patients. Hepatocellular carcinoma (HCC) is a common malignancy with poor clinical outcome. The high incidence of HCC might be related to expo-sure to known risk factors, including carcinogenic compounds, such as N-nitrosamines, which cause DNA damage. N-nitrosamines affect cell mitochondrial metabolism, disturbing the balance between reactive oxygen species (ROS) and antioxidants, causing oxidative stress and DNA damage, potentially leading to carcinogenesis. This work addresses the progressive histological changes in the liver of N-diethylnitrosamine (DEN)-exposed mice and its correlation with oxidative stress.Male ICR mice were randomly divided into five DEN-exposed and five matched control groups. DEN was IP administered, once a week, for eight consecutive weeks.2 Samples were taken 18 h after the last DEN injection (8 weeks post-exposure). The following sampling occurred at weeks 15th, 22nd, 29th and 36th after the first DEN injection. DEN resulted in early toxic lesions and, from week 29 onwards, in progressive proliferative lesions. Between 15 and 29 weeks, DEN-exposed animals showed significant changes in hepatic antioxidant (glutathione, glutathione reductase, and catalase) status (p < 0.05) compared with controls. These results point to an association between increased DEN-induced oxidative stress and the early histopathological alterations, suggesting that DEN disrupted the antioxidant defense mechanism, thereby triggering liver carcinogenesis.

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Section: Discussionsupporting

confidence: 77%

N-diethylnitrosamine mouse hepatotoxicity: Time-related effects on histology and oxidative stress

Santos

Colaço

Costa

et al. 2014

Experimental and Toxicologic Pathology

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“…Our result agrees well with that [33][34] who reported that elevated levels of serum AST, ALT, ALP, LDH and γ-GT, and simultaneous fall in the levels of the marker enzymes in the liver tissue induced by DEN was altered after the administration of ethanolic leaf extract of the medicinal plant Cassia fistula Linn. The structural integrity of the cells has been damaged in cancer induced animals, and this results in cytoplasmic leakage of the enzyme into the blood stream, leads to the elevated levels of these enzymes in blood with a subsequent fall in the tissues [35].…”

Section: Resultssupporting

confidence: 83%

Attenuation of DEN induced hepatocellular carcinoma by a novel synthesis of silver nanoparticles using Solanum villosum (Mill.) leaves

Venkatesh¹,

Krishnasamy²

2016

JBT

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Objective: In today's fast and furious life, the life style and food habits of people are changing drastically, due to which ninety percent of the population suffers with oxidative stress resulting as ageing process, arthritis, ischemia, neurodegeneration, chronic liver diseases and cancer. The aim of the study is to evaluate the anticancer potential of Solanum villosum leaves against the diethylnitrosamine induced experimental hepatocellular carcinoma in male wistar albino rats.Methods:Various biochemical, cancer markers, intermediatery enzymes and histopathology were studied systematically for different groups of rats. Results:The animals exposed to diethylnitrosamine showed significant alterations in all biochemical and metabolic enzymes. After administration of silver nanoparticles of Solanum villosum leaves (SNPs-AESVL 100 µg/kg b.w) effectively suppressed the tumor growth induced by diethylnitrosamine. Conclusion:Our findings suggest that silver nanoparticles of Solanum villosum leaves are potential anticancer effect, preventing tumor growth and hepatocellular damage.

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“…It is reported that DEN cause oxidative stress during the metabolism that lead to cytotoxicity, mutagenicity and carcinogenicity (3,4). DEN is biotransformed by mixed-function cytochrome P450 dependent monooxidase systems and its metabolic activation is responsible for the onset of the toxic effects (5). So the use of antioxidants offer to protect this deleterious effects.…”

Section: Introductionmentioning

confidence: 99%

Effects of Echinacea purpurea on Hepatic and Renal Toxicity Induced by Diethylnitrosamine in Rats

Rezaie

Fazlara

Karamolah

et al. 2013

Jundishapur J Nat Pharm Prod

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Background: Nitrites are mainly used in food preservation. These materials could change to nitrosamine due to the effect of heat and gastric acid. Nitrosamine is absorbed in intestine and enters the liver and hepatocytes by portal venous system, and hampers the detoxification system of liver by interfering in cytochrome P450 enzymes, so, the liver gently proceeds to cirrhosis and cancer. Objectives: The current study aimed to investigate the hepatic and renal protective effects of aerial parts of Echinacea purpurea extract (EPE) on injury induced by diethylnitrosamine (DEN). Materials and Methods: Twenty Wistar rats were divided into 4 groups. Groups were as follow: Control group (normal saline), DEN (200 mg/ kg, IP, a single dose), EPE (100 mg/kg, orally, daily) and DEN + EPE which received as group DEN and EPE. After 30 days, Blood samples, and liver and kidney tissues were taken for further examination. Aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), BUN, Creatinine and total and direct bilirubin were estimated in serum. Results: DEN induced hepatotoxicity and nephrotoxicity in all the treated animals by elevated serum ALT, AST, ALP and BUN, creatinin and total and direct bilirubin levels. AST, BUN and total and direct bilirubin significantly decreased in DEN + EPE compared to DEN group. After 30 days of DEN administration, histopathological investigation revealed proliferation of hepatic stellate cells and early fibrosis which were partly improved by EPE administration. Conclusions: The current study findings indicated that Echinacea purpurea extract played an important role in the protection against DEN toxicity in rats.

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Protective effect of Cassia fistula Linn. on diethylnitrosamine induced hepatocellular damage and oxidative stress in ethanol pretreated rats

Cited by 48 publications

References 39 publications

N-diethylnitrosamine mouse hepatotoxicity: Time-related effects on histology and oxidative stress

N-diethylnitrosamine mouse hepatotoxicity: Time-related effects on histology and oxidative stress

Attenuation of DEN induced hepatocellular carcinoma by a novel synthesis of silver nanoparticles using Solanum villosum (Mill.) leaves

Effects of Echinacea purpurea on Hepatic and Renal Toxicity Induced by Diethylnitrosamine in Rats

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