Protective Effect of Carnobacterium spp. against Listeria monocytogenes during Host Cell Invasion Using In vitro HT29 Model

Abstract

The pathogenesis of listeriosis results mainly from the ability of Listeria monocytogenes to attach, invade, replicate and survive within various cell types in mammalian tissues. In this work, the effect of two bacteriocin-producing Carnobacterium (C. divergens V41 and C. maltaromaticum V1) and three non-bacteriocinogenic strains: (C. divergens V41C9, C. divergens 2763, and C. maltaromaticum 2762) was investigated on the reduction of L. monocytogenes Scott A plaque-forming during human infection using the HT-29 in vitro model. All Carnobacteria tested resulted in a reduction in the epithelial cell invasion caused by L. monocytogenes Scott A. To understand better the mechanism underlying the level of L. monocytogenes infection inhibition by Carnobacteria, infection assays from various pretreatments of Carnobacteria were assessed. The results revealed the influence of bacteriocin production combined with a passive mechanism of mammalian cell monolayers protection by Carnobacteria. These initial results showing a reduction in L. monocytogenes virulence on epithelial cells by Carnobacteria would be worthwhile analyzing further as a promising probiotic tool for human health.

Bioprotective Effect of Lactococcus piscium CNCM I-4031 Against Listeria monocytogenes Growth and Virulence

“…A slight but a significant decrease in virulence was also observed for the ScottA strain, suggesting that the effect of the protective bacteria on L. monocytogenes virulence is strain-dependent. The effect on virulence reduction by LAB has already been described for foodborne pathogens such as L. monocytogenes ( Garriga et al, 2015 ; Pilchova et al, 2016 ) or Campylobacter ( Alemka et al, 2010 ). The effect is usually investigated as one of the probiotic properties of bacteria; however, such additional properties also increase the bioprotective value of strains and their safety assessment.…”

Cannabinoid control of gingival immune activation in chronically SIV-infected rhesus macaques involves modulation of the indoleamine-2,3-dioxygenase-1 pathway and salivary microbiome

“…In vitro studies have demonstrated the ability of Carnobacteriaceae, a group of bacteriocin producing lactic acid bacteria to reduce the virulence of Listeria monocytogenes on colonic epithelial cells. 78 Further, members of Carnobacteriaceae were proven to be effective as a probiotic in food-animal production 79 , 80 , 81 suggesting that they may exert a similar effect on the oral epithelial barrier. Furthermore, members of the family Bifidobacteriaceae are part of the oral commensal bacteria known to exert beneficial effects in the oral cavity and are currently being used as probiotics.…”

Exploring the cytotoxic mechanisms of Pediocin PA-1 towards HeLa and HT29 cells by comparison to known bacteriocins: Microcin E492, enterocin heterodimer and Divercin V41

“…Enterocin also displays cytotoxic effects towards HT29 and HeLa cells, as a heterodimer these effects were greater. Enterocin also displayed a greater effect towards HeLa than HT29 cells [ 14 , 35 ].…”