Protective Effect of Calendula officinalis L. Flowers Against Monosodium Glutamate Induced Oxidative Stress and Excitotoxic Brain Damage in Rats

Shivasharan, B. D.; Nagakannan, P.; Thippeswamy, B.S.; Veerapur, Veeresh P.

doi:10.1007/s12291-012-0256-1

Cited by 77 publications

(53 citation statements)

References 26 publications

(28 reference statements)

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“…In the present study, administration of L-arginine produced increased nitrosative stress substantiated by increased pancreatic nitrate/nitrite at all time points. Studies have reported that C. officinalis possesses potential NO radical scavenging property (Ahmad et al 2012;Shivasharan et al 2013). Our study shows COE treatment markedly decreased nitrite/nitrate levels.…”

Section: Discussionsupporting

confidence: 61%

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

Kaur

Sidhu

Chopra

et al. 2016

Pharmaceutical Biology

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Context: Calendula officinalis L. (Asteraceae) has been traditionally used in treating inflammation of internal organs, gastrointestinal tract ulcers and wound healing. Objective: The present study investigates the effect of ethanol extract (95%) of Calendula officinalis flowers in L-arginine induced acute necrotizing pancreatitis in rats. Materials and methods: Rats were divided into four groups: normal control, L-arginine control, Calendula officinalis extract (COE) treated and melatonin treated (positive control), which were further divided into subgroups (24 h, day 3 and 14) according to time points. Two injections of L-arginine 2 g/kg i.p. at 1 h intervals were administered in L-arginine control, COE and melatonin-treated groups to produce acute necrotizing pancreatitis. Biochemical parameters [serum amylase, lipase, pancreatic amylase, nucleic acid content, total proteins, transforming growth factor-b1 (TGF-b1), collagen content, lipid peroxidation, reduced glutathione and nitrite/nitrate] and histopathological studies were carried out. Results: COE treatment (400 mg/kg p.o.) was found to be beneficial. This was evidenced by significantly lowered histopathological scores (2 at day 14). Nucleic acid content (DNA 21.1 and RNA 5.44 mg/g pancreas), total proteins (0.66 mg/mL pancreas) and pancreatic amylase (1031.3 100 SU/g pancreas) were significantly improved. Marked reduction in pancreatic oxidative and nitrosative stress; collagen (122 lmoles/ 100 mg pancreas) and TGF-b1 (118.56 pg/mL) levels were noted. Results obtained were comparable to those of positive control. Discussion and conclusion: The beneficial effect of COE may be attributed to its antioxidant, antinitrosative and antifibrotic actions. Hence, the study concludes that COE promotes spontaneous repair and regeneration of the pancreas. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 61%

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

Kaur

Sidhu

Chopra

et al. 2016

Pharmaceutical Biology

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“…Previous studies report that MSG causes severe behavioral abnormalities such as increased irritability, hypoactivity, and deficits in spontaneous alternation behavior [22]. In agreement with the above reports, CUR may attenuate the extracellular glutamate concentration at safe physiological level which may be by a number of buffering mechanisms include uptake of glutamate by glial cells and its conversion by glutamine synthetase or glutamate decarboxylase to the nontoxic glutamine [23].…”

Section: Discussionsupporting

confidence: 85%

Curcumin Protects against Monosodium Glutamate Neurotoxicity and Decreasing NMDA2B and mGluR5 Expression in Rat Hippocampus

Khalil

Khedr

2016

Neurosignals

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Background: Monosodium glutamate (MSG) is a flavor enhancer used in food industries. MSG is well documented to induce neurotoxicity. Curcumin (CUR) reportedly possesses beneficial effects against various neurotoxic insults. Hence, this present study has been designed to evaluate the neuroprotective effect of curcumin on MSG-induced neurotoxicity in rats. Methods: Thirty-two male Wister rats were divided into four groups (n=8): Control group, MSG group, CUR group and MSG + CUR group. CUR (Curcumin 150 mg/kg, orally) was given day after day for four weeks along with MSG (4 mg/kg, orally). After 4 weeks, rats were sacrificed and brain hippocampus was isolated immediately on ice. Inflammatory marker TNFα and acetylcholinesterase (AChE) activity (marker for cholinergic function) were estimated. Gene expressions of metabotropic glutamate receptor 5 (mGluR5) and N-methyl-D-aspartate receptor 2B (NMDA2B) along with glutamate concentration were assessed. Results: Treatment with CUR significantly attenuated AChE activity and TNFα in MSG-treated animals. The anti-inflammatory properties of CUR may be responsible for this observed neuroprotective action. A possible role of CUR to attenuate both glutamate level and gene expression of NMDA2B and mGLUR5 in brain hippocampus was established when compared to MSG group. Conclusion: We concluded that CUR as flavor enhancer protects against MSG-induced neurotoxicity in rats.

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“…Glutamate is the primary excitatory neurotransmitter in the mammalian central nervous system. Despite this key role, glutamate may be toxic to neuron in case it is in excess (Shivasharan et al, 2013). In the brain, it binds to its receptors, which are N-methyl D-aspartate (NMDA), α -amino-3-hydroxyl-5-methyl-4-isoxaleprioprionic acid (AMPA), and Kainate receptors.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Properties of Dichrocephala Integrifolia (Asteraceae) in a Mouse Model of Monosodium Glutamate-Induced Neurotoxicity

Nadège¹,

Sylviane²,

Agathe³

et al. 2017

Afr J Tradit Complement Altern Med

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Background: In Africa, neurodegenerative diseases in the elderly have become a major health concern due to the increase in live expectancy. Glutamate mediated neurotoxicity is involved in neurodegenerative diseases such as Ischemia, Epilepsy, Alzheimer's and Parkinson diseases. Plants with antioxidant properties are reported to protect vital organs against glutamate toxicity. This study aims to assess the effect of Dichrocephala integrifolia against monosodium glutamatemediated neurotoxicity and oxidative stress. Methodology: The decoction prepared from the leaves of Dichrocephala integrifolia was evaluated against monosodium glutamate-induced neurotoxicity in mice. The animals were grouped in seven groups of 6 animals each. The animals received daily; distilled water (p.o) for the distilled water and the negative control groups, one of the four doses of the decoction of the plant (35, 87.5, 175 or 350 mg/kg p.o) for the tests groups and memantine (20 mg/kg p.o) for the positive control group. Monosodium glutamate (2.5 g/kg ip) was injected daily to animals except those of the normal control group all the seven days of the experimentation. Animals were observed for aggressiveness, locomotor and forepaws muscle grip activities 30 min after monosodium injections. Brain reduced glutathione and malondialdehyde levels were also assessed following the behavioral tests on day 8. Results: The decoction of Dichrocephala integrifolia at the doses of 87.5 and 175 mg/kg significantly (p<0.01) inhibited the aggressiveness of monosodium treated mice, significantly (p<0.01) counteracted the reduction in locomotor and forepaws muscle grip capacity induced by monosodium glutamate. Furthermore, the decreases in reduced glutathione level and increases in lipid peroxidation level induced by monosodium glutamate were significantly (p<0.001) reversed by Dichrocephala integrifolia at the doses of 87.5 and 175 mg/kg. Conclusion:The results of this study reveal that Dichrocephala integrifolia is a medicinal plant that protects the brain against monosodium glutamate-mediated neurotoxicity. This can explain why this plant is intensively used in folk medicine in Cameroon to prevent and treat some central nervous system illnesses.

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Protective Effect of Calendula officinalis L. Flowers Against Monosodium Glutamate Induced Oxidative Stress and Excitotoxic Brain Damage in Rats

Cited by 77 publications

References 26 publications

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

Calendula officinalisamelioratesl-arginine-induced acute necrotizing pancreatitis in rats

Curcumin Protects against Monosodium Glutamate Neurotoxicity and Decreasing NMDA2B and mGluR5 Expression in Rat Hippocampus

Antioxidant Properties of Dichrocephala Integrifolia (Asteraceae) in a Mouse Model of Monosodium Glutamate-Induced Neurotoxicity

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