Protective effect of Bojungikki-tang, a traditional herbal formula, against alcohol-induced gastric injury in rats

Lee, Mee-Young; Shin, In‐Sik; Jeon, Woo‐Young; Seo, Chang‐Seob; Ha, Hyekyung; Huh, Jung-Im; Shin, Hyeun‐Kyoo

doi:10.1016/j.jep.2012.04.043

Cited by 39 publications

(33 citation statements)

References 43 publications

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“…The extent of gastric mucosal injury was evaluated using light microscopy by an experienced histologist who was blinded to the treatment regimen. The histopathological changes were assessed to previousely described criteria [21]. …”

Section: Methodsmentioning

confidence: 99%

Banhabaekchulchunma-tang, a traditional herbal formula attenuates absolute ethanol-induced gastric injury by enhancing the antioxidant status

Shin

Jeon

Shin

et al. 2013

BMC Complement Altern Med

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BackgroundBanhabaekchulchunma-tang (hange-byakujutsu-tenma-to in Japanese and banxia-baizhu-tianma-tang in Chinese) is a mixture of fourteen herbs. It is used traditionally for the treatment of anemia, anorexia, general weakness, and female infertility in China, Japan, and Korea. In this study, we investigated the protective effects of a Banhabaekchulchunma-tang water extract (BCT) against ethanol-induced acute gastric injury in rats.MethodsGastric injury was induced by intragastric administration of 5 mL/kg body weight of absolute ethanol to each rat. The positive control group and the BCT group were given oral doses of omeprazole (50 mg/kg) or BCT (400 mg/kg), respectively, 2 h prior to the administration of absolute ethanol. The stomach of each animal was excised and examined for gastric mucosal lesions. To confirm the protective effects of BCT, we evaluated the degree of lipid peroxidation, the level of reduced glutathione (GSH), and the activities of the antioxidant enzymes catalase, glutathione-S-transferase, glutathione peroxidase, and glutathione reductase in the stomach. In addition, we conducted an acute toxicity study to evaluate the safety of BCT according to OECD guideline.ResultsBCT reduced ethanol-induced hemorrhage, hyperemia, and loss of epithelial cell in the gastric mucosa. BCT reduced the increased lipid peroxidation associated with ethanol-induced acute gastric lesions, and increased the mucosal GSH content and the activities of antioxidant enzymes. In addition, BCT did not cause any adverse effects at up to 5000 mg/kg.ConclusionsThese results indicate that BCT protects the gastric mucosa against ethanol-induced gastric injury by increasing the antioxidant status. We suggest that BCT could be developed as an effective drug for the treatment of gastric injury caused by alcohol intake.

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Section: Methodsmentioning

confidence: 99%

Banhabaekchulchunma-tang, a traditional herbal formula attenuates absolute ethanol-induced gastric injury by enhancing the antioxidant status

Shin

Jeon

Shin

et al. 2013

BMC Complement Altern Med

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“…Tissues were subsequently mounted and coverslipped using Dako mounting medium (Invitrogen Cooperation, CA, USA). The histopathological findings were assessed according to the criteria, as described previously [19, 20]. …”

Section: Methodsmentioning

confidence: 99%

Protective Effects of the Traditional Herbal Formula Oryeongsan Water Extract on Ethanol-Induced Acute Gastric Mucosal Injury in Rats

Jeon

Lee

Shin

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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This study was performed to evaluate the protective effect and safety of Oryeongsan water extract (OSWE) on ethanol-induced acute gastric mucosal injury and an acute toxicity study in rats. Acute gastric lesions were induced via intragastric oral administration of absolute ethanol at a dose of 5 mL/kg. OSWE (100 and 200 mg/kg) was administered to rats 2 h prior to the oral administration of absolute ethanol. The stomach of animal models was opened and gastric mucosal lesions were examined. Gastric mucosal injuries were evaluated by measuring the levels of malondialdehyde (MDA), glutathione (GSH), and the activity of antioxidant enzymes. In the acute toxicity study, no adverse effects of OSWE were observed at doses up to 2000 mg/kg/day. Administration of OSWE reduced the damage by conditioning the gastric mucosa against ethanol-induced acute gastric injury, which included hemorrhage, hyperemia, and loss of epithelial cells. The level of MDA was reduced in OSWE-treated groups compared with the ethanol-induced group. Moreover, the level of GSH and the activity of antioxidant enzymes were significantly increased in the OSWE-treated groups. Our findings suggest that OSWE has a protective effect on the gastric mucosa against ethanol-induced acute gastric injury via the upregulation of antioxidant enzymes.

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“…Catalase controls the accumulation of ROS generated through numerous metabolic processes such as the breakdown of ethanol in the stomach. Inhibition of catalase activity leads to lipid formation by increasing the generation of hydroxyl radicals (Lee et al, 2012). The biochemical function of GSH is to reduce lipid hydroperoxides to their corresponding alcohols and free hydrogen peroxide to water, hence plays an important role in cell protection (Nguyen et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Gastroprotective and antioxidant potentials of ethanolic stem bark extract of Margaritaria discoidea (Euphorbiaceae) in rats

Sofidiya

Orisaremi

Sansaliyu

et al. 2015

Journal of Ethnopharmacology

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Protective effect of Bojungikki-tang, a traditional herbal formula, against alcohol-induced gastric injury in rats

Cited by 39 publications

References 43 publications

Banhabaekchulchunma-tang, a traditional herbal formula attenuates absolute ethanol-induced gastric injury by enhancing the antioxidant status

Banhabaekchulchunma-tang, a traditional herbal formula attenuates absolute ethanol-induced gastric injury by enhancing the antioxidant status

Protective Effects of the Traditional Herbal Formula Oryeongsan Water Extract on Ethanol-Induced Acute Gastric Mucosal Injury in Rats

Gastroprotective and antioxidant potentials of ethanolic stem bark extract of Margaritaria discoidea (Euphorbiaceae) in rats

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