Protective effect of Blumea lacera DC aerial parts in indomethacin-induced enterocolitis in rats

Abstract

Traditionally Blumea lacera DC is used to treat inflammation and bowel ailments. Lack of specific, curative treatment for IBD enticed us to investigate the therapeutic efficacy of ethanolic extract of aerial parts of Blumea lacera DC (EEBL) against indomethacin-induced enterocolitis. Male Wistar rats were divided into six groups (n = 5) and different doses of EEBL (100 and 200 mg/kg, p.o) and sulphasalazine (100 mg/kg, p.o) were administered for seven days. Enterocolitis was induced by two subsequent doses of indomethacin (7.5 mg/kg, s.c) on 7th and 8th day. Treatments were continued up to 12th day and sacrificed. The protective effect was assessed on the basis of macroscopic scores of ileum strips, changes in biochemical parameters such as serum lactate dehydrogenase (LDH), tissue myeloperoxidase (MPO), lipid peroxidation (LPO), and total thiols (TT). Further, activity was ascertained by histopathological evaluations. HPLC fingerprinting profiling of EEBL was also carried out. Pre-treatment with EEBL or sulphasalazine significantly attenuated the indomethacin-induced proximal ileal damage, elevated levels of serum LDH, tissue MPO, LPO and lower levels of TT. Further, observed activity of EEBL was well correlated with histopathological alterations. The results revealed the protective action of the title plant against the indomethacin-induced enterocolitis in rats, which might be attributed by its antioxidant, anti-inflammatory, antimicrobial, and membrane-stabilizing properties.

Development of phytosterol-loaded silver nanoparticles for ameliorating haemorrhoidal complications via the AMPK pathway—a mechanistic approach

“…The current investigation has also shown considerably increased serum concentrations of cytokines including TNF-α and IL-6 (Gurel et al 2013, Azeemuddin et al 2014, and severity score analysis corroborated this. After receiving therapy with PLSNPs, the levels of these cytokines in the animals' serum decreased, confirming its ability to reduce inflammation, which is essential for any potential haemorrhoid treatment (Basnet et al 2015).…”

Optimization of Extraction Process and Anti-Hemorrhoidal Activity of Blumea lacera (Burm.f.) DC. Leaves in Croton Oil-induced Hemorrhoid Model

“…In the same manner, the present study also showed significantly higher levels of cytokines like TNF-α and IL-6 in serum on COP application, which compliments the earlier research outcomes (Azeemuddin et al, 2014; Gurel et al, 2013) and was also confirmed by severity score analysis. The decrease in the levels of these cytokines in serum after treatment with EBL affirmed the anti-inflammatory role of EBL, which is very essential for any candidate to have anti-hemorrhoid potential (Basnet et al, 2015).…”

Ginsenoside Rd therapy improves histological and functional recovery in a rat model of inflammatory bowel disease

“…The rat IBD models were established by treatment with indomethacin (Basnet, Adhikari, Sachidananda, Thippeswamy, & Veerapur, 2015). Four of the rats were killed 4 days after indomethacin administration, and the intestinal tissue was removed without perfusion to confirm injury to the gastrointestinal tract.…”

“…The rat IBD models were established by treatment with indomethacin (Basnet, Adhikari, Sachidananda, Thippeswamy, & Veerapur, 2015).…”