Protective Effect of Blue-M1 against Oxidative Stress on COS-1 Cells

Usui, Teruyuki; Shizuuchi, Satomi; Watanabe, Hisahiko; Hayase, Fumitaka

doi:10.1271/bbb.68.247

Cited by 10 publications

(8 citation statements)

References 6 publications

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“…Blue‐M1, blue‐M2, red‐M1, and red‐M2 showed strong antioxidative activities as well as melanoidins 7. Especially, blue‐M1 showed a protective effect against oxidative stress on COS‐1 cells 8Figure 4. indicates that melanoidins induced expression of IFN‐γ but not IL‐4 in spleen cells exposed with ovalbumin as allergen.…”

Section: Resultsmentioning

confidence: 98%

Chemistry and Biological Effects of Melanoidins and Glyceraldehyde‐Derived Pyridinium as Advanced Glycation End Products

Hayase

Usui

Nishiyama

et al. 2005

Annals of the New York Academy of Sciences

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Blue pigments (blue-M1 and blue-M2) and red pigments (red-M1 and red-M2) were generated in a xylose-glycine reaction system. Blue-M2 was identified as an addition compound of di-xylulose-glycine to blue-M1 that involved two pyrrolopyrrole structures. We identified red pigments as isomers of addition compounds of xylulose-glycine to the condensed compound between pyrrolopyrrole-2-carbaldehyde and pyrrole-2-carbaldehyde. These pigments have polymerizing activity, suggesting that they are important Maillard reaction intermediates through the formation of melanoidins. Melanoidins induced IFN-gamma and IL-12 expression in spleen cells exposed to allergen and in macrophages, respectively. These findings suggest that melanoidins have a suppressive effect on allergic reaction as a novel physiological effect. On the other hand, we identified a glyceraldehyde-derived advanced glycation end product (AGE) formed from glyceraldehyde and N-acetylarginine as well as glyceraldehyde-derived pyridinium (GLAP) in physiological conditions. The AGE was identified as 5-methylimidazoline-4-one (MG-H1), which has been reported to be formed from arginine and methylglyoxal. GLAP, which induced reactive oxygen species (ROS) production in HL-60 cells, is supposed to be a toxic AGE, while MG-H1 is a nontoxic AGE.

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Section: Resultsmentioning

confidence: 98%

Chemistry and Biological Effects of Melanoidins and Glyceraldehyde‐Derived Pyridinium as Advanced Glycation End Products

Hayase

Usui

Nishiyama

et al. 2005

Annals of the New York Academy of Sciences

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“…19) Our studies indicate that Blue-M1 had hydroxyl and DPPH radical scavenging activity, 20) and had a physiologically protective effect against oxidative stress on COS-1 cells. 21) This indicates that Blue-M1 has the same functions as melanoidins. It can be also assumed that the novel red pigments in this study have the same antioxidative effects.…”

Section: Generation Of Red Pigments and Polymerization Mechanismmentioning

confidence: 96%

Identification of Red Pigments Formed in aD-Xylose-glycine Reaction System

Shirahashi¹,

Watanabe²,

Hayase³

2009

Bioscience, Biotechnology, and Biochemistry

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“…We assessed the protective effect of Blue-M1 against AAPH-induced cytotoxicity on COS-1 cells [21]. Cell viability was measured with tetrazolium salt, WST-8.…”

Section: Effects Of Blue-m1 Against Cytotoxicity On Cos-1 Cellsmentioning

confidence: 99%

“…These results suggest that Blue-M1 prevents the oxidative cell injury. Therefore, Blue-M1 will be an antioxidant, which protects against the oxidative stress in biological systems [21].…”

Section: Effects Of Blue-m1 Against Cytotoxicity On Cos-1 Cellsmentioning

confidence: 99%

Chemistry and some biological effects of model melanoidins and pigments as Maillard intermediates

Hayase

Usui

Watanabe

2006

Molecular Nutrition Food Res

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Various pigments were formed in the D-xylose-glycine reaction system. Blue pigments (Blue-M1 and Blue-M2) and red pigments (Red-M1 and Red-M2) were generated in the Maillard reaction. Blue-M2 is presented to have been generated by the reaction between Blue-M1, which involved two pyrrolopyrrole structures as the major blue pigment, and di-D-xyluloseglycine. We identified red pigments as the isomers of addition compounds of D-xyluloseglycine to condensated compound between pyrroropyrrole-2-carbaldehyde and pyrrole-2-carbaldehyde compounds. These pigments have polymerizing activities, suggesting that they are important Maillard reaction intermediates through the formation of melanoidins. Blue-M1 as well as melanoidins effectively suppressed the peroxidation of linoleic acid. The scavenging activity toward Blue-M1 on hydroxyl and DPPH radicals was also as strong as that of melanoidins. Furthermore, Blue-M1 prevents the oxidative cell injury. Therefore, Blue-M1 will be an antioxidant which protects against the oxidative stress in biological systems. Melanoidins induced IFN-gamma mRNA and IL-12 mRNA expressions in spleen cells exposed to allergen and in macrophage-like J774.1 cells, respectively. These findings suggest that melanoidins have suppressive effect on allergic reaction as a novel physiological effect.

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Protective Effect of Blue-M1 against Oxidative Stress on COS-1 Cells

Cited by 10 publications

References 6 publications

Chemistry and Biological Effects of Melanoidins and Glyceraldehyde‐Derived Pyridinium as Advanced Glycation End Products

Chemistry and Biological Effects of Melanoidins and Glyceraldehyde‐Derived Pyridinium as Advanced Glycation End Products

Identification of Red Pigments Formed in aD-Xylose-glycine Reaction System

Chemistry and some biological effects of model melanoidins and pigments as Maillard intermediates

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