Protective Effect of Bergenin against Cyclophosphamide-Induced Immunosuppression by Immunomodulatory Effect and Antioxidation in Balb/c Mice

Qi, Qiuchen; Dong, Zhen; Sun, Yehuan; Li, Siying; Zhao, Zhongxi

doi:10.3390/molecules23102668

Cited by 56 publications

(41 citation statements)

References 47 publications

(58 reference statements)

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“…TCM monomers and their derivatives are believed to be very helpful in resolution of inflammatory responses, and their bioactive function and mechanism have been explored in recent years. As a representative TCM monomer, Bergenia purpurascens Qi Q., et al, 2018 which is a potent compound extracted from Bergenia purpurascens, has been demonstrated to exert numerous pharmacological effects, including antibacterial, analgesic, anti-inflammatory and antitumor activities ( Fisher et al, 2017 ; Wu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection

et al. 2021

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Background:Klebsiella pneumonia has emerged as a critical pathogen causing severe clinical problems, such as pneumonia and sepsis. Meanwhile, intensified drug resistance induced by antibiotic therapy necessitates discovering novel and active molecules from Traditional Chinese Medicine (TCM) for treatment.Methods and results: In this study, the isolated Bergenin monohydrate showed an anti-inflammatory effect in Klebsiella-infected mice. We initially investigated the anti-inflammatory effects and cytoprotection against oxidative stress in vitro and in vivo. Interestingly, a specific dose of Bm can effectively ameliorate lung injury and suppress the expression of inflammatory cytokines such as TNF-α, IL-6, IL-1β and PEG2. Moreover, Bm was also shown to reduced the levels of MPO, MDA and increased SOD and GSH activities. Moreover, we assessed the intracellular signaling molecules including p38, ERK, JNK, IκB, NF-κB-p65 by western blotting and verified through MAPK and NF-κB pathways inhibition experiments. These results reveal that Bm executed its effects via the classical MAPK signaling pathway and NF-κB pathway.Conclusion: Given its underlying anti-inflammatory effect, Bm may be used as a promising therapeutic against Klebsiella-induced infection, thus providing a benefit for the future clinical therapy of pneumonia and medicine design.

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Section: Discussionmentioning

confidence: 99%

Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection

et al. 2021

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“…Phosphoramide mustard, one of these compounds, binds to DNA and prevents it from replicating and then stops cancer cells from proliferating. 4,7,8 Different from other regions of the brain, the cerebellum undergoes its major developing period from the third trimester to infant stage in humans. 5 In spite of its wide usage, CTX causes a wide range of adverse effects including immunosuppression, 6 bone marrow suppression, gonadal toxicity, leucopaenia and oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Histological, cellular and behavioural analyses of effects of chemotherapeutic agent cyclophosphamide in the developing cerebellum

Luo

Tang

et al. 2019

Cell Proliferation

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Objectives:We performed histological, cellular and behavioural analyses of the effects of cyclophosphamide (CTX), a chemotherapeutic drug, in the developing cerebellum and aimed to provide valuable insights into clinical application of CTX in children.Materials and methods: C57BL/6 mice and Math1-dependent GFP expression transgenic mice were used in the research. H&E staining was performed to analyse histological effects of CTX in the cerebellum. Staining for EdU and TUNEL was used to estimate the cell proliferation and apoptosis. Rotarod test and hanging wire test were used to evaluate the behavioural functions. Immunofluorescent staining was used to identify the cell types. The differentiation markers and genes related to Sonic Hedgehog (SHH) signalling were measured via quantitative real-time PCR or immunoblotting. Results:We found that while CTX induced a significant reduction in cell proliferation and increased apoptosis in the EGL in 48 hours, the behavioural functions and the multilayer laminar structure of cerebella were largely restored when the mice grew to adults. Mechanistically, granule neuron progenitors, driven by the SHH signalling, enhanced the capability of proliferation quickly after CTX administration was stopped, which allowed the developing cerebellum to catch up and to gradually replenish the injury. Conclusion:The chemotherapeutic agent CTX induces an immediate damage to the developing cerebellum, but the cerebellar multilayer laminar structure and motor function can be largely restored if the agent is stopped shortly after use.

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“…The surplus of electrons is then transferred to oxygen molecules, generating superoxide (O 2 - ), which in turn activates damaging pathways and gives rise to other ROS in a chain reaction [ 32 ]. Previous studies have shown that bergenin diminishes intracellular levels of superoxide and other ROS by increasing the activity of antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (Gpx) [ 12 , 16 , 25 , 26 ]. In this work, the antioxidant activity of bergenin was confirmed both in vitro, by the inhibition of NO production by J774 macrophages and in vivo, by the reduction of MDA and nitrite levels in neuropathic mice’s sciatic nerve and spinal cord.…”

Section: Discussionmentioning

confidence: 99%

“…Bergenin ( Figure 1 ) is a naturally occurring C-glucoside of 4-O-methylgallic acid found in many plant species. Several therapeutic applications of bergenin have been proposed in the literature, e.g., anti-ulcerative [ 10 ], hepatoprotective [ 11 , 12 ], anticancer [ 13 ], neuroprotective [ 14 , 15 ], antidiabetic [ 16 ], anti-inflammatory [ 17 , 18 , 19 , 20 , 21 , 22 ], antinociceptive [ 17 , 23 ], and antioxidant [ 12 , 16 , 24 , 25 , 26 , 27 ]. Considering that oxidative stress and neuroinflammation play key roles in the pathophysiology of DN [ 28 ], the above-mentioned properties of bergenin may contribute to the effective control of painful DN.…”

Section: Introductionmentioning

confidence: 99%

Bergenin Reduces Experimental Painful Diabetic Neuropathy by Restoring Redox and Immune Homeostasis in the Nervous System

Villarreal

Santos

Lauria

et al. 2020

IJMS

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Diabetic neuropathy is a frequent complication of diabetes. Symptoms include neuropathic pain and sensory alterations—no effective treatments are currently available. This work characterized the therapeutic effect of bergenin in a mouse (C57/BL6) model of streptozotocin-induced painful diabetic neuropathy. Nociceptive thresholds were assessed by the von Frey test. Cytokines, antioxidant genes, and oxidative stress markers were measured in nervous tissues by ELISA, RT-qPCR, and biochemical analyses. Single (3.125–25 mg/kg) or multiple (25 mg/kg; twice a day for 14 days) treatments with bergenin reduced the behavioral signs of diabetic neuropathy in mice. Bergenin reduced both nitric oxide (NO) production in vitro and malondialdehyde (MDA)/nitrite amounts in vivo. These antioxidant properties can be attributed to the modulation of gene expression by the downregulation of inducible nitric oxide synthase (iNOS) and upregulation of glutathione peroxidase and Nrf2 in the nervous system. Bergenin also modulated the pro- and anti-inflammatory cytokines production in neuropathic mice. The long-lasting antinociceptive effect induced by bergenin in neuropathic mice, was associated with a shift of the cytokine balance toward anti-inflammatory predominance and upregulation of antioxidant pathways, favoring the reestablishment of redox and immune homeostasis in the nervous system. These results point to the therapeutic potential of bergenin in the treatment of painful diabetic neuropathy.

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Protective Effect of Bergenin against Cyclophosphamide-Induced Immunosuppression by Immunomodulatory Effect and Antioxidation in Balb/c Mice

Cited by 56 publications

References 47 publications

Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection

Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-κB Pathways Against Klebsiella pneumonia Infection

Histological, cellular and behavioural analyses of effects of chemotherapeutic agent cyclophosphamide in the developing cerebellum

Bergenin Reduces Experimental Painful Diabetic Neuropathy by Restoring Redox and Immune Homeostasis in the Nervous System

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