Protective effect of berberine on doxorubicin-induced acute hepatorenal toxicity in rats

Abstract

Doxorubicin (DOX), a potent broad‑spectrum chemotherapeutic agent used for the treatment of several types of cancer, is largely limited due to its serious side effects on non‑target organs. Thus, the present study aimed to investigate whether berberine (Ber), an isoquinoline alkaloid, could reduce DOX‑induced acute hepatorenal toxicity in rats. Fifty rats were randomly divided into five groups: i) Control group, ii) DOX group, iii) DOX+Ber (5 mg kg) group; iv) DOX+Ber (10 mg kg), and v) DOX+Ber (20 mg kg) group. In the tests, body weight, organ index, general condition and mortality were observed. In addition, the serum levels of alanine transaminase (ALT), aspartate aminotransferase (AST), total cholesterol (TCHO) and blood urea nitrogen (BUN) were determined to evaluate hepatorenal function. Hepatorenal toxicity was further assessed using hematoxylin and eosin stained sections. Furthermore, the levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and malondialdehyde (MDA) in rat serum or tissue homogenate were also assessed to determine the mechanisms of action. Results suggested that pretreatment with Ber ameliorated the DOX‑induced liver and kidney injury by lowering the serum ALT, AST, TCHO and BUN levels, and the damage observed histologically, such as hemorrhage and focal necrosis of liver and kidney tissues induced by DOX were also attenuated by Ber. Furthermore, Ber also exerted certain antioxidative properties through reversing the changes in the levels of MDA, SOD, GSH and MDA induced by DOX. These findings indicate that Ber has protective effects against DOX‑induced acute hepatorenal toxicity in rats. Combination of Ber with DOX is a novel strategy that has the potential for protecting against DOX‑induced hepatorenal toxicity in clinical practice.

Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway

“…BBR has been reported to have biochemical and pharmacological effects such as antidiabetic, anticancer, anti-fibrotic, antibacterial, antioxidant, and anti-inflammatory (11)(12)(13)(14)(15). Recent studies have reported that BBR ameliorates renal conditions such as podocyte injury, nephrotoxicity, acute hepato-renal toxicity, and type 2 diabetic nephropathy (9,(16)(17)(18). Additionally, BBR inhibits…”

Protective effect of berberine on aconite‑induced myocardial injury and the associated mechanisms

“…Ber is extracted from Rhizoma coptidis , and the latter has also been used to treat diabetes mellitus in China for >1,400 years ( 14 ). Additionally, it has also been demonstrated that Ber alleviates the acute cardiotoxicity and hepatorenal toxicity resulting from doxorubicin treatment in rats ( 15 , 16 ). However, to the best of our knowledge, no study to date has been performed concerning the protective effects of Ber on aconite-induced myocardial toxicity.…”

Protective effect of berberine on renal fibrosis caused by diabetic nephropathy

“…Berberine (BBR) is a type of isoquinolone alkaloid that is extracted from the widely used Chinese herb, Rhizoma coptidis . Recent studies have indicated that BBR has multiple pharmacological activities, including hypolipidemic, antioxidant and glucose-lowering, suggesting that it may have clinical potential as an alternative therapeutic drug for diabetic complications ( 8 , 9 ). Liu et al ( 10 ) reported that BBR effectively lowered blood glucose and lipid levels through suppression of oxidative stress.…”