Protective effect of Bacillus subtilis, B. pumilus, and Pseudomonas fluorescens isolates against root knot nematode Meloidogyne incognita on cowpea

Abd-El-Khair, H.; El-Nagdi, W. M. A.; Youssef, M. M. A.; Abd-Elgawad, Mahfouz M. M.; Dawood, Mona

doi:10.1186/s42269-019-0108-8

Cited by 41 publications

(23 citation statements)

References 14 publications

(11 reference statements)

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“…The present study also depicted that inoculation of P. aeruginosa (M1) and B. gladioli (M2) improved the morphological characters of the plants inhabited with PPNs. Our studies are in the agreement to previous studies conducted by Abd-El-Khair et al [64], who reported that biocontrol agents Bacillus subtilis, B. pumilus and P. fluorescens had a protective role against PPN M. incognita in improving root length, shoot length, fresh and dry weights in cowpea. According to their assumptions, the biocontrol agents have the stimulating effects on plants by inducing growth, plant nutrition, producing plant hormones and suppressing various pathogens in the surrounding zones [64].…”

Section: Discussionsupporting

confidence: 93%

Impact of Plant Growth Promoting Rhizobacteria in the Orchestration of Lycopersicon esculentum Mill. Resistance to Plant Parasitic Nematodes: A Metabolomic Approach to Evaluate Defense Responses Under Field Conditions

et al. 2019

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The present study deals with biological control of Meloidogyne incognita in 45-days old Lycopersicon esculentum, inoculated with Pseudomonas aeruginosa(M1) and Burkholderia gladioli (M2). The improved plant growth and biomass of nematode infested Plant growth promoting rhizobacteria (PGPR) inoculated plants was observed. Remarkable reduction in the numbers of second stage juvenile (J2s), root galls was recorded after treatment of microbes relative to experimental controls. Moreover, the lowered activities of oxidative stress markers (H2O2 (hydrogen peroxide), O2− (superoxide anion), malondialdehyde (MDA)) was estimated in plants after rhizobacterial supplementation. Higher activities of enzymatic (SOD (Superoxide dismutase), POD (Guaiacol peroxidase), CAT (Catalase), GPOX (Glutathione peroxidase), APOX (Ascorbate peroxidase), GST (Glutathione-S-transferase), GR (Glutathione reductase), DHAR (Dehydroascorbate reductase), PPO (Polyphenol oxidase)) and non-enzymatic (glutathione, ascorbic acid, tocopherol) antioxidants were further determined in nematode infected plants following the addition of bacterial strains. The upregulation of photosynthetic activities were depicted by evaluating plant pigments and gas exchange attributes. An increase in the levels of phenolic compounds (total phenols, flavonoids, anthocyanins), osmoprotectants (total osmolytes, carbohydrates, reducing sugars, trehalose, proline, glycine betaine, free amino acids) and organic acids (fumaric, succinic, citric, malic acid) were reflected in infected plants, showing further enhancement after application of biocontrol agents. The study revealed the understanding of plant metabolism, along with the initiative to commercially exploit the biocontrol agents as an alternative to chemical nematicides in infected fields for sustainable agriculture.

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Section: Discussionsupporting

confidence: 93%

Impact of Plant Growth Promoting Rhizobacteria in the Orchestration of Lycopersicon esculentum Mill. Resistance to Plant Parasitic Nematodes: A Metabolomic Approach to Evaluate Defense Responses Under Field Conditions

et al. 2019

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“…Bacillus subtilis and B. pumilus caused the highest reduction (82% and 81.8%, respectively) in M. incognita on cowpea [ 32 ]. An isolate of Bacillus megaterium reduced the root penetration and migration of M. graminicola to between 40 and 60% compared with non–treated roots of rice plants [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Bacterial Microbiota Isolated from Cysts of Globodera rostochiensis (Nematoda: Heteroderidae)

Oro

Knežević²,

Dinić³

et al. 2020

Plants

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The potato cyst nematode (PCN) Globodera rostochiensis is a plant parasite of potato classified into a group of quarantine organisms causing high economic losses worldwide. Due to the long persistence of the parasite in soil, cysts harbor numerous bacteria whose presence can lead to cyst death and population decline. The cysts of G. rostochiensis found in two potato fields were used as a source of bacteria. The universal procedure was applied to extract DNA from bacteria which was then sequenced with 16S primers. The aims of the study were to identify bacterial microbiota associated with the PCN populations and to infer their phylogenetic relationships based on the maximum likelihood and Bayesian phylogeny of the 16S sequences. In addition, the impact of the most significant climate and edaphic factors on bacterial diversity were evaluated. Regarding the higher taxonomy, our results indicate that the prevalent bacterial classes were Bacilli, Actinobacteria and Alphaproteobacteria. Phylogenetic analyses clustered Brevibacterium frigoritolerans within the family Bacillaceae, confirming its recent reclassification. Long-term climate factors, such as air temperature, insolation hours, humidity and precipitation, as well as the content of soil organic matter, affected the bacterial diversity. The ability of cyst nematodes to persist in soil for a long time qualifies them as a significant natural source to explore the soil bacterial microbiota.

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“…Nematicides provide very effective and faster results by reducing nematode numbers and reproduction rates, but they are not environmentally friendly and have many drawbacks (Abd-El-Khair et al, 2019).…”

Section: Yield (Pod)-related Parametersmentioning

confidence: 99%

“…Trichoderma spp. are basically mycoparasites (Papavizas, 1985), but in recent years, their suppressive effects against plant nematodes have also been reported (Siddiqui and Shaukat, 2004;Javeed et al, 2016;Abd El-Khair et al,2019). Soils rich in organic matter are generally colonized by some biocontrol agents such as T. harzianum that improve biocontrol activity.…”

Section: Introductionmentioning

confidence: 99%

Effect of certain organic amendments and Trichoderma species on the root-knot nematode, Meloidogyne incognita, infecting pea (Pisum sativum L.) plants

El-Nagdi

Youssef

Abd-El-Khair

et al. 2019

Egypt J Biol Pest Control

Self Cite

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This study was conducted to evaluate the effects of three fungal species, Trichoderma harzianum (Th), T. viride (Tv), and T. vierns (Tvi), and ground seeds of fennel (Foeniculum vierns) and caraway (Carum carvi), and powdered leaves of basil (Ocimum basilicum) as soil amendments against the root-knot nematode, Meloidogyne incognita on pea root, Pisum sativum under screen house conditions. The treatments were compared to a nematicide, carbofuran 10G, and untreated check. The tested materials significantly (P ≤ 0.05) reduced M. incognita on pea as indicated by the numbers of galls, egg masses, and J 2 per root system in pots, either as single or combined treatments. The combined treatments caused higher average total percentages of nematode reduction than those achieved by single ones. The single treatments recorded the averages total percentages of nematode reduction ranged from 72.4 to 82.0%. Basil waste as single was superior in reducing nematode numbers of J 2 and egg masses in root system per plant, as it achieved the highest average total percentages of nematode reduction (77.9%) than 73. 9 and 72.4% caused by fennel and caraway, respectively. In combined treatments, the average total percentages of nematode reduction ranged from 72.4 to 86.4%. Basil + (Th) caused the highest reduction (86.4%), followed by caraway+Tvi (86.3%) and caraway+Th (86.2%). The nematicide, carbofuran was as effective as most as the treatments in reducing nematode parameters. Also, the treatments highly increased growth and yield criteria of pea infected with M. incognita as indicated by shoot length and fresh and dry weights and root fresh weight, number and fresh and dry weights of pods.

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Protective effect of Bacillus subtilis, B. pumilus, and Pseudomonas fluorescens isolates against root knot nematode Meloidogyne incognita on cowpea

Cited by 41 publications

References 14 publications

Impact of Plant Growth Promoting Rhizobacteria in the Orchestration of Lycopersicon esculentum Mill. Resistance to Plant Parasitic Nematodes: A Metabolomic Approach to Evaluate Defense Responses Under Field Conditions

Impact of Plant Growth Promoting Rhizobacteria in the Orchestration of Lycopersicon esculentum Mill. Resistance to Plant Parasitic Nematodes: A Metabolomic Approach to Evaluate Defense Responses Under Field Conditions

Bacterial Microbiota Isolated from Cysts of Globodera rostochiensis (Nematoda: Heteroderidae)

Effect of certain organic amendments and Trichoderma species on the root-knot nematode, Meloidogyne incognita, infecting pea (Pisum sativum L.) plants

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