Protective effect of Astragaloside IV to inhibit thiram-induced tibial dyschondroplasia

Mehmood, Khalid; Zhang, Hui; Yao, Wangyuan; Jiang, Xiong; Waqas, Muhammad; Li, Aoyun; Wang, Yaping; Lei, Li; Zhang, Lihong; Qamar, Hammad; Li, Jiakui

doi:10.1007/s11356-019-05032-1

Cited by 17 publications

(20 citation statements)

References 28 publications

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“…Grape seed extract supplementation reduced the TD lesion in growth plate cartilage significantly and decreased the TD score and proper gait and normal standing ability was restored. These outcomes were also reported by former scholars (Iqbal et al, 2018;Mehmood et al, 2019b).…”

Section: Discussionsupporting

confidence: 82%

Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract through Downregulating CA2 Gene and Enhancing Liver Functions

Qamar¹

2019

PVJ

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Globally Tibial Dyschondroplasia (TD) is a serious metabolic syndrome in broilers that is identified by the accretion of the growth plate (GP) into the tibialmetaphyseal portion. Grape seed extract (GSE) is a well-known significant antioxidant since it possesses multifaceted anti-inflammatory, anti-cancer and reducing reactive oxygen ability. The experiment was intended to evaluate the proficiency of GSE to recover the chicks affected with TD. For this purpose, 210 chicks were bought and fed normal starter feed for the first 3 days. After that two groups were made having 70 (Control group; normal feed) and 140 chickens (Thiram group; normal feed+ thiram @ 50 mg/kg). Further two equal divisions were made in Thiram group on 7 th day viz TD group and GSE group (@ 50 mg/kg/day). GSE significantly (P<0.05) reverted the blood serum profile and liver antioxidants metabolites and, reduced the histopathological abrasion of the liver as compared to the TD group. Furthermore, GSE chicks exhibited a significant decrease (P<0.05) in CA2 gene expression and less mortality, TD score and GP size. In inference, GSE is a natural plant-based extract which owns solid scavenging and bone healing property, and strong antioxidant capacity. GSE provided recovery to chicks affected with TD chicks via dropping TD score, whereas stabilized the GP width through the CA2 mRNA downregulation. ©2019 PVJ. All rights reservedKey words: Broiler Carbonic anhydrase Liver oxidative stress Tibial dyschondroplasia lesion Toxicity To Cite This Article: Qamar H, Mehmood K, Li A, Waqas M, Iqbal M, Zhang L, Tong X and Li J, 2019. Recovery of chickens affected with tibial dyschondroplasia by application of grape seed extract through downregulating ca2 gene and enhancing liver functions. Pak Vet J, 39(4): 527-533. http://dx.

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Section: Discussionsupporting

confidence: 82%

Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract through Downregulating CA2 Gene and Enhancing Liver Functions

Qamar¹

2019

PVJ

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“…Conversely, after PTE administration, the chickens in the PTE group became alert and healthy, and performance indices were significantly improved (p < 0.05) on day 18 in comparison with the TD group. The TD chickens showed poor growth performance due to the reduced feed intake, weight gain, difficulty in movement and standing as in agreement with other literature [50]. After the administration of PTE, chickens not only become alert and healthy but also improved the feed intake, body weight, and feed conversion ratio.…”

Section: Discussionsupporting

confidence: 89%

“…The cDNA was synthesized using a commercial kit (first-strand reverse transcription cDNA kit, Tian Gen, China) according to the manufacturer's specifications. A 20 µL, the reaction mixture containing oligo (dT)18, 2× reaction Mix, and 5 µL RNA was used for reverse transcription in a thermocycler (Applied Biosystem CA, USA) at the reaction temperature of 42 • C for 30 min and 85 • C for 5 min [50]. Primer Premier Sofware version 5.0 (Wuhan biotechnology Co. Ltd., Wuhan, China) was used to design the gene specific primers from already reported sequences for respective genes [39] (Table 1).…”

Section: Rna Extraction and Rt-qpcrmentioning

confidence: 99%

Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling

Qamar

Waqas

et al. 2019

Biomolecules

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Tibial dyschondroplasia (TD) negatively affects broilers all over the world, in which the accretion of the growth plate (GP) develops into tibial proximal metaphysis. Plastrum testudinis extract (PTE) is renowned as a powerful antioxidant, anti-inflammatory, and bone healing agent. The current study was conducted to evaluate the efficacy of PTE for the treatment of thiram-induced TD chickens. Broilers (day old; n = 300) were raised for 3 days with normal feed. On the 4th day, three groups (n = 100 each) were sorted, namely, the control (normal diet), TD, and PTE groups (normal diet+ thiram 50 mg/kg). On the 7th day, thiram was stopped in the TD and PTE group, and the PTE group received a normal diet and PTE (30 mg/kg/day). Plastrum testudinis extract significantly restored (p < 0.05) the liver antioxidant enzymes, inflammatory cytokines, serum biochemicals, GP width, and tibia weight as compared to the TD group. The PTE administration significantly increased (p < 0.05) growth performance, vascularization, AKT (serine/threonine-protein kinase), and PI3K expressions and the number of hepatocytes and chondrocytes with intact nuclei were enhanced. In conclusion, PTE has the potential to heal TD lesions and act as an antioxidant and anti-inflammatory drug in chickens exposed to thiram via the upregulation of AKT and PI3K expressions.

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“…The schematic experiment design is explained in Figure 2 [32]. An equal number of chickens were incorporated into three groups designated as control, TD, and osthole groups (n = 80).…”

Section: Methodsmentioning

confidence: 99%

Osthole: A Coumarin Derivative Assuage Thiram-Induced Tibial Dyschondroplasia by Regulating BMP-2 and RUNX-2 Expressions in Chickens

Waqas

Wang

et al. 2019

Antioxidants

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Avian tibial dyschondroplasia affects fast growing broiler chickens accounting for almost 30% of leg ailments in broilers. The present project was designed to assess the efficacy of osthole against avian tibial dyschondroplasia (TD). Two hundred and forty chickens were equally allocated into control, TD and osthole groups (n = 80). The TD and osthole group chickens were challenged with tetramethylthiuram disulfide (thiram) at 50 mg/kg of feed from 4–7 days, followed by osthole administration at 20 mg/kg orally to the osthole group only from 8–18 days. Thiram feeding resulted in lameness, increased mortality, and decreased production parameters, alkaline phosphatase (ALP), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and glutathione peroxidase (GSH-PX) levels, along with significantly increased aspartate aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA) levels, and growth plate size. Moreover, the genes and protein expressions of BMP-2 and RUNX-2 were significantly down-regulated in TD affected chickens (p < 0.05). Osthole administration showed promising results by alleviating lameness; increased ALP, SOD, T-AOC, and GSH-Px levels; and decreased the AST, ALT, and MDA levels significantly. It restored the size of the growth plate and significantly up-regulated the BMP-2 and RUNX-2 expressions (p < 0.05). In conclusion, the oxidative stress and growth plate anomalies could be assuaged using osthole.

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Protective effect of Astragaloside IV to inhibit thiram-induced tibial dyschondroplasia

Cited by 17 publications

References 28 publications

Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract through Downregulating CA2 Gene and Enhancing Liver Functions

Recovery of Chickens Affected with Tibial Dyschondroplasia by Application of Grape Seed Extract through Downregulating CA2 Gene and Enhancing Liver Functions

Plastrum Testudinis Extract Mitigates Thiram Toxicity in Broilers via Regulating PI3K/AKT Signaling

Osthole: A Coumarin Derivative Assuage Thiram-Induced Tibial Dyschondroplasia by Regulating BMP-2 and RUNX-2 Expressions in Chickens

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