Protective Effect of Aqueous Neem Leaf Extract on Erythromycin Induced Histomorphological Changes on Hepatocytes of (Albino Wistar) Rats

Abstract

Objective: To determine the protective effect of aqueous Neem leaf extract-induced hepatotoxicity by Erythromycin.Study Design: Laboratory-based Experimental study.Place and Duration of Study: The study was conducted at the Department of Anatomy and Animal House of Baqai Medical University (BMU) Karachi, Pakistan from January 2019 to March 2019.Materials and Methods: A total of 80 albino Wistar male rats were randomly divided into 4 groups. Control group, marked as Group A with no interventions. Only Erythromycin was administered to the rats in group B. Erythromycin and an aqueous extract of neemleaves were administered to group C rats. Group D only received an extract of aqueous neemleaves. Animals' body weight was noted both before and after experiments. Liver weight was also measured after 14 days when the animals were sacrificed.Results: Histopathological changes in group B include loss of architecture, portal vein observed with congestion, and hemorrhages. In Group C, histopathological changes showed hepatocytes with slightly disturbed and mild congestion with decreased sinusoidal spaces; however, Group A andGroup D observed normal hepatocyte architecture. Conclusion: According to the current study, Erythromycin-induced oxidative stress and hepatocyte damage weremarkedly reduced by aqueous neem leaf extract, which is a powerful antioxidant.