Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa

Chi, Hee-Jun; Kim, J.H.; Ryu, C.S.; Lee, J.Y.; Park, J.S.; Chung, D.Y.; Choi, S.Y.; Kim, M.H.; Chun, Eunyoung; Roh, Sangho

doi:10.1093/humrep/den060

Cited by 107 publications

(73 citation statements)

References 33 publications

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“…[93][94][95][96] In contrast, other studies have reported that antioxidants (e.g., glutathione and catalase) are ineffective in protecting spermatozoa from the loss of motility during sperm processing. [97][98][99] It is important to note that sperm samples from infertile men may be more susceptible to oxidative injury (from semen processing) and be afforded greater protection by antioxidants than samples from fertile men. 92 Antioxidants appear to be of limited value in protecting sperm DNA from gentle semen processing (e.g., incubation or densitygradient centrifugation) ( Table 5).…”

Section: Role Of In Vitro Antioxidants In Protecting Spermatozoa Frommentioning

confidence: 99%

“…92 Antioxidants appear to be of limited value in protecting sperm DNA from gentle semen processing (e.g., incubation or densitygradient centrifugation) ( Table 5). [98][99][100][101] In some cases, antioxidants supplementation in vitro (e.g., combination of vitamins C and E) may cause sperm DNA damage. 99,101 ROLE OF IN VITRO ANTIOXIDANTS IN PROTECTING SPERMATOZOA FROM CRYOPRESERVATION AND THAWING Several studies have evaluated the role of antioxidants in protecting spermatozoa from the loss of motility that occurs following cryopreservation and thawing.…”

Section: Role Of In Vitro Antioxidants In Protecting Spermatozoa Frommentioning

confidence: 99%

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Antioxidant therapy in male infertility: fact or fiction?

Zini¹,

Alhathal²

2011

Asian J Androl

110

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Infertile men have higher levels of semen reactive oxygen species (ROS) than do fertile men. High levels of semen ROS can cause sperm dysfunction, sperm DNA damage and reduced male reproductive potential. This observation has led clinicians to treat infertile men with antioxidant supplements. The purpose of this article is to discuss the rationale for antioxidant therapy in infertile men and to evaluate the data on the efficacy of dietary and in vitro antioxidant preparations on sperm function and DNA damage. To date, most clinical studies suggest that dietary antioxidant supplements are beneficial in terms of improving sperm function and DNA integrity. However, the exact mechanism of action of dietary antioxidants and the optimal dietary supplement have not been established. Moreover, most of the clinical studies are small and few have evaluated pregnancy rates. A beneficial effect of in vitro antioxidant supplements in protecting spermatozoa from exogenous oxidants has been demonstrated in most studies; however, the effect of these antioxidants in protecting sperm from endogenous ROS, gentle sperm processing and cryopreservation has not been established conclusively. Keywords: antioxidant; male infertility; oxidative stress; sperm DNA fragmentation; vitamins RELATIONSHIP BETWEEN OXIDATIVE STRESS AND SPERM DYSFUNCTION Seminal oxidative stress (OS) results from an imbalance between reactive oxygen species (ROS) production and ROS scavenging by seminal antioxidants. Seminal OS is believed to be one of the main factors in the pathogenesis of sperm dysfunction and sperm DNA damage in male infertility.1-4 Indeed, it is estimated that 25% of infertile men possess high levels of semen ROS, whereas fertile men do not have high levels of semen ROS.1,4-6 Although a controlled production of these ROS is required for sperm physiology (sperm hyperactivation, capacitation and acrosome reaction) and for natural fertilization, [7][8][9] the excessive production of ROS by immature germ cells and leukocytes causes sperm dysfunction (lipid peroxidation, loss of motility and sperm DNA damage). 9,10 Spermatozoa are particularly susceptible to oxidative injury due to the abundance of plasma membrane polyunsaturated fatty acids. [10][11][12] These unsaturated fatty acids provide fluidity that is necessary for membrane fusion events (e.g., the acrosome reaction and sperm-egg interaction) and for sperm motility. However, the unsaturated nature of these molecules predisposes them to free radical attack and ongoing lipid peroxidation throughout the sperm plasma membrane. Once this process has been initiated, accumulation of lipid peroxides occurs on the sperm surface (this results in loss of sperm motility) and oxidative damage to DNA can ensue. 13,14SEMINAL ANTIOXIDANT CAPACITY AND SPERM DYSFUNCTION Seminal plasma and spermatozoa themselves are well endowed with an array of protective antioxidants to protect spermatozoa from OS, especially, at the post-testicular level. 6,15,16 Seminal plasma contains a number of high-molecular weigh...

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Section: Role Of In Vitro Antioxidants In Protecting Spermatozoa Frommentioning

confidence: 99%

Section: Role Of In Vitro Antioxidants In Protecting Spermatozoa Frommentioning

confidence: 99%

Antioxidant therapy in male infertility: fact or fiction?

Zini¹,

Alhathal²

2011

Asian J Androl

110

View full text Add to dashboard Cite

show abstract

“…In general, antioxidants appear to be of limited value in protecting sperm DNA from gentle semen processing (e.g. incubation or density-gradient centrifugation) (see Table 4) [62][63][64][65]. In some cases, antioxidants supplementation in vitro (e.g.…”

Section: In Vitro Antioxidants and Sperm Dna Damagementioning

confidence: 99%

Antioxidants and sperm DNA damage: a clinical perspective

Zini

Gabriel

Baazeem

2009

J Assist Reprod Genet

175

110

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Purpose Infertile men possess substantially more sperm DNA damage than do fertile men, damage that may impact negatively on reproductive outcomes. In this era of assisted reproductive technologies there is mounting concern regarding the safety of utilizing DNA-damaged spermatozoa in this setting. Therefore, it is important to identify strategies that may reduce sperm DNA damage. The purpose of this review is to discuss the rationale for antioxidant therapy in men with sperm DNA damage and to evaluate the data on the efficacy of dietary and in vitro antioxidant preparations on sperm DNA damage. Methods We reviewed the literature on antioxidants and sperm DNA damage. Results To date, the data suggest that dietary antioxidants may be beneficial in reducing sperm DNA damage, particularly, in men with high levels of DNA fragmentation. However, the mechanism of action of dietary antioxidants has not been established and most of the clinical studies are small. A beneficial effect of in vitro antioxidant supplements in protecting sperm DNA from exogenous oxidants has been demonstrated, however, the effect of these antioxidants in protecting sperm from endogenous ROS, gentle sperm processing and cryopreservation has not been established.

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“…Superoxide dismutase and catalase were shown to improve sperm survival, reduce ROS generation in boar spermatozoa and prevent human sperm membrane lipid peroxidation during freeze-thaw procedures [12,13]. Catalase had pronounced effects on improving the post-thaw quality of canine spermatozoa and the overall functional parameters of human spermatozoa [14,15]. Glutathione peroxidase activity was shown to be important against lipid peroxidation in human spermatozoa because lipid peroxidation increased significantly either through the inhibition of glutathione peroxidase action or by depleting glutathione availability [16].…”

Section: Introductionmentioning

confidence: 99%

The protective effects of α-ketoacids against oxidative stress on rat spermatozoa in vitro

Li¹,

Liu²,

Zhang³

et al. 2009

Asian J Androl

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The aim of this study was to determine the effects of antioxidants, including a-ketoacids (a-ketoglutarate and pyruvate), lactate and glutamate/malate combination, against oxidative stress on rat spermatozoa. Our results showed that ) significantly enhanced tyrosine-phosphorylated proteins with the size of 95 kDa (P ≤ 0.04). At the same time, a-ketoglutarate, pyruvate, lactate, glutamate and malate supplemented in media can be used as important energy sources and supply ATP for sperm motility. In conclusion, the present results show that a-ketoacids could be effective antioxidants for protecting rat spermatozoa from H 2 O 2 attack and could be effective components to improve the antioxidant capacity of Biggers, Whitten and Whittingham media.

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Protective effect of antioxidant supplementation in sperm-preparation medium against oxidative stress in human spermatozoa

Abstract: Supplementing sperm preparation medium with EDTA or catalase significantly improved the overall functional parameters of the spermatozoa by reducing the ROS levels.

Cited by 107 publications

References 33 publications

Antioxidant therapy in male infertility: fact or fiction?

Antioxidant therapy in male infertility: fact or fiction?

Antioxidants and sperm DNA damage: a clinical perspective

The protective effects of α-ketoacids against oxidative stress on rat spermatozoa in vitro

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