Protective effect of Anneslea fragrans ethanolic extract against CCl4-induced liver injury by inhibiting inflammatory response, oxidative stress and apoptosis

Wang, Yudan; Deng, Xiaocui; Liu, Yaping; Wang, Yifen; Luo, Xiaodong; Zhao, Ting; Wang, Zhengxuan; Cheng, Guiguang

doi:10.1016/j.fct.2023.113752

Cited by 8 publications

(5 citation statements)

References 64 publications

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Section: Discussionmentioning

confidence: 70%

“…An intraperitoneal injection of CCl4 can result in liver inflammation and oxidative stress through the downregulation of superoxide dismutase and GSH [30]. CCl 4 increased plasma ALT and AST activities and induced hepatic fibrosis formation by decreasing α-SMA and collagen proteins [31]. We found that significant changes in ALT, AST, DBIL, TBIL, and liver structures occurred in the CCl 4 -treated rats; these results are supported by those of Ebaid [32], who showed that CCl 4 significantly upregulated the levels of lipid peroxidation (MDA), cholesterol, LDL, and bilirubin but significantly suppressed the activity of GSH.…”

Section: Discussionmentioning

confidence: 99%

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Dimethyl Bisphenolate Ameliorates Carbon Tetrachloride-Induced Liver Injury by Regulating Oxidative Stress-Related Genes

Wang,

Shen,

Zhang

et al. 2023

Molecules

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Liver disease accounts for millions of deaths per year all over the world due to complications from cirrhosis and liver injury. In this study, a novel compound, dimethyl bisphenolate (DMB), was synthesized to investigate its role in ameliorating carbon tetrachloride (CCl4)-induced liver injury through the regulation of oxidative stress-related genes. The structure of DMB was confirmed based on its hydrogen spectrum and mass spectrometry. DMB significantly reduced the high levels of ALT, AST, DBIL, TBIL, ALP, and LDH in a dose-dependent manner in the sera of CCl4-treated rats. The protective effects of DMB on biochemical indicators were similar to those of silymarin. The ROS fluorescence intensity increased in CCl4-treated cells but significantly weakened in DMB-treated cells compared with the controls. DMB significantly increased the content of oxidative stress-related GSH, Nrf2, and GCLC dose-dependently but reduced MDA levels in CCl4-treated cells or the liver tissues of CCl4-treated rats. Moreover, DMB treatment decreased the expression levels of P53 and Bax but increased those of Bcl2. In summary, DMB demonstrated protective effects on CCl4-induced liver injury by regulating oxidative stress-related genes.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Dimethyl Bisphenolate Ameliorates Carbon Tetrachloride-Induced Liver Injury by Regulating Oxidative Stress-Related Genes

Wang,

Shen,

Zhang

et al. 2023

Molecules

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“…The bioactivities of natural plants are dependent on their chemical compositions [ 18 , 19 , 20 ]. More than 2300 compounds have been identified ( Supplementary Material Table S1 ) in DH extract, and these chemical compounds could be classified into over eighteen different types according to their structures ( Figure 1 A).…”

Section: Resultsmentioning

confidence: 99%

“…Phenolics and flavones are specific substances among natural products, which show bioactivity in many studies [ 17 , 19 , 21 ]. Thereby, the levels of total phenolic content (TPC) and total flavone content (TFC) were measured in this study.…”

Section: Resultsmentioning

confidence: 99%

A Comparative Analysis of Chemical Constituents and Antioxidant Effects of Dendrobium fimbriatum Hook Fractions with Different Polarities

Zhao

Liu

et al. 2023

IJMS

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The aim of this study was to investigate the chemical composition and antioxidant capacity of various polar fractions obtained from Dendrobium fimbriatum Hook (DH). First, a 90% ethanol-aqueous extract of DH (CF) was subjected to sequential fractionation using different organic solvents, resulting in the isolation of a methylene chloride fraction (DF), an ethyl acetate fraction (EF), an n-butanol fraction (BF), and a remaining water fraction (WF) after condensation. Additionally, the CF was also subjected to column chromatography via a D101 macroreticular resin column, eluted with ethanol-aqueous solution to yield six fractions (0%, 20%, 40%, 60%, 80%, and 100%). UPLC-Q-Exactive Orbitrap-MS/MS analysis identified a total of 47 chemical compounds from these polar fractions, including fatty acids, amino acids, phenolic acids, flavonoids, organic heterocyclic molecules, and aromatic compounds. Moreover, DF, EF, and the 60%, 80%, and 100% ethanol-aqueous fractions had higher total phenol content (TPC) and total flavonoid content (TFC) values and greater 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS-) and 1,1-diphenyl-2-picrylhydrazyl (DPPH)-scavenging abilities. In H2O2-induced HepG2 cells, the aforementioned fractions could increase the activities of antioxidative enzymes NAD(P)H: quinone oxidoreductase 1 (NQO1), superoxide dismutase (SOD), heme oxygenase-1 (HO-1) and catalase (CAT), stimulate glutathione (GSH) synthesis by increasing the activities of glutamic acid cysteine ligase (GCL) and glutathione synthetase (GS), regulate GSH metabolism by increasing glutathione peroxidase (GSH-Px) and glutathione reductase (GR) activities, and reduce levels of reactive oxygen species (ROS) and malondialdehyde (MDA). Furthermore, the antioxidative stress effect of the DH fractions was found to be positively correlated with the activation of nuclear factor-erythroid 2-related factor 2 (Nrf2) protein and the presence of antioxidative chemical constituents. In conclusion, this study highlights the efficacy of both liquid–liquid extraction and macroporous resin purification techniques in the enrichment of bioactive compounds from natural food resources. The comprehensive analysis of chemical constituents and antioxidant effects of different polar fractions from Dendrobium fimbriatum Hook contributes to the understanding of its potential application in functional foods and nutraceuticals.

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“…TUNEL staining was performed to visualize apoptotic cells in the liver tissue. 16 The deparaffinized and dehydrated liver sections were treated with proteinase K solution (15 min, 37 °C) and then incubated with TUNEL and DAPI reagents (Wuhan Servicebio Technology Co., Ltd, China). The results were detected using a panoramic MIDI digital slide scanner, in which blue and green fluorescence indicated the nuclei and apoptotic cells, respectively.…”

Section: Methodsmentioning

confidence: 99%

Comparison of the hepatoprotection of intragastric and intravenous cyanidin-3-glucoside administration: focus on the key metabolites and gut microbiota modulation

Wang,

Tang,

Mao

et al. 2024

Food Funct.

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Protective effect of Anneslea fragrans ethanolic extract against CCl4-induced liver injury by inhibiting inflammatory response, oxidative stress and apoptosis

Cited by 8 publications

References 64 publications

Dimethyl Bisphenolate Ameliorates Carbon Tetrachloride-Induced Liver Injury by Regulating Oxidative Stress-Related Genes

Dimethyl Bisphenolate Ameliorates Carbon Tetrachloride-Induced Liver Injury by Regulating Oxidative Stress-Related Genes

A Comparative Analysis of Chemical Constituents and Antioxidant Effects of Dendrobium fimbriatum Hook Fractions with Different Polarities

Comparison of the hepatoprotection of intragastric and intravenous cyanidin-3-glucoside administration: focus on the key metabolites and gut microbiota modulation

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