Protective Effect of Acteoside on Ovariectomy-Induced Bone Loss in Mice

Yang, Lingling; Zhang, Bo; Liu, Jingjing; Dong, Yanhong; Li, Yanting; Li, Nan; Zhao, Xiaojun; Snooks, Hunter; Hu, Changling; Ma, Xueqin

doi:10.3390/ijms20122974

Cited by 30 publications

(15 citation statements)

References 31 publications

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“…Furthermore, VB suppresses RANKL‐mediated osteoclastogenesis by inhibiting c‐Fos and NF‐κB pathways 146 . Daily oral administration of VB enhances bone mineral density and ameliorates biochemical parameters of bone resorption indicators as well as bone formation index 147 …”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Cistanches Herba, from an endangered species to a big brand of Chinese medicine

Song

Zeng

Jiang

et al. 2021

Medicinal Research Reviews

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Cistanches Herba (CH, Chinese name: Roucongrong), is a very precious, tonic Chinese medicine. Cistanche deserticola and Cistanche tubulosa are the two commonly used species and authenticated in Chinese Pharmacopoeia. Due to the parasitic nature of Cistanche plants, the wild source was once endangered and listed in the Appendix II of Convention on International Trade in Endangered Species of Wild Fauna and Flora. However, after continuously struggling in the past decades, CH has grown up to a big brand of Chinese medicine featured with the cultivation area as 1.26 million mu, the annual output as 6000 tons, and the related industrial output value as more than 20 billion China Yuan, attributing to large‐scale cultivation and in‐depth phytochemical and pharmacological investigations. Noteworthily, great achievements have reached concerning the research and development of relevant products, such as modern drugs, traditional Chinese medicine prescriptions, and dietary supplements. The current review summarizes the research progresses concerning the distribution and cultivation, phytochemistry, pharmacology, metabolism and product development of CH in the past decades, and the emerging challenges and developing prospects are discussed as well.

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Section: Pharmacological Activitiesmentioning

confidence: 99%

Cistanches Herba, from an endangered species to a big brand of Chinese medicine

Song

Zeng

Jiang

et al. 2021

Medicinal Research Reviews

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“…And, ginsenoside rb3 defends cardiomyocytes against oxidative injury via activation of PERK/Nrf2/HMOX1 axis [37]. Acteoside signi cantly in uenced osteoclastogenesis by downregulated NFKBIA expression to prevent the development of osteoporosis [38]. Most of these genes have been linked to the treatment of mechanism of OP.…”

Section: The Potential Key Targetsmentioning

confidence: 99%

A network pharmacology approach to explore and validate the potential targets underlying the effect of Ginsenoside on Osteoporosis

Zhen

Ling

Zhen

et al. 2021

Preprint

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Objective This study aim to investigate the potential targets involving the effect of ginsenoside on osteoporosis using a network pharmacology approach. Methods Ginsenoside and its drug targets associated to osteoporosis (OP) were identified by using network analysis. First, the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP), DrugBank database, Pharmmapper database and Cytoscape software were used to mine information relevant to Ginsenoside ingredients and Ginsenoside -related targets. Second, the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of ginsenoside-target gene and ginsenoside-OP target gene were performed in String, Phenopedia, DisGeNET database and Metascape software. Eventually, the protein to protein interaction (PPI) of key ginsenoside-OP targets were created by String database and Cytoscape software. The validation of the binding of ginsenoside to target proteins was plotted by utilizing SwissDock tool, UCSF Chimera and Pymol software. Results A total of 8 important active ingredients of ginsenosides were obtained in the TCMSP. Eighty potential targets of ginsenoside and 1304 related targets involved in OP were subjected to network analysis, and the 17 intersection targets were indicated to be linked to ginsenoside treating OP. GO and KEGG analysis showed the top 10 items of biological processes, cellular components, molecular functions and signaling pathways in the 80 targets of ginsenoside. Then, 14 key targets were determined to be the most crucial genes by protein to protein interaction (PPI) analysis. In the 14 intersection potential targets, 10 signaling pathways were defined by Metascape software. Validation plots of four target proteins IL1B, TNF, IFNG, NFKBIA binding to ginsenoside rh2 was drew, lastly. Conclusion This study investigated the potential targets and signaling pathways of ginsenoside during the treatment of OP, which might be beneficial to elucidate the mechanism concerned to the action of ginsenoside and might supply a better understanding of its anti-OP effects.

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“…Moreover, osteoporosis is a chronic disease and it can threaten people's health, reduce activities of daily living and worsen quality of life. Generally, females are more prone to osteoporosis than males especially aged women after menopause (Yang et al, 2019). Postmenopausal osteoporosis (PMOP) is associated with the lack of estrogen after menopause (Miyauchi et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rg3 attenuates ovariectomy‐induced osteoporosis via AMPK/mTOR signaling pathway

Zhang

Huang

Chen

et al. 2020

Drug Development Research

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Ginsenoside Rg3, a ginsenoside isolated from Panax ginseng, can regulate autophagy via AMP‐activated protein kinase/mammalian target of rapamycin (AMPK/mTOR) signaling pathway. AMPK/mTOR signaling and autophagy have been reported to be involved in osteogenesis. Here, the effect of Rg3 on ovariectomy (OVX)‐induced osteoporosis is explored. In vivo, rats were treated with 20 mg/kg Rg3 after OVX and the body weight (BW) was monitored. Bone mineral density (BMD), hematoxylin–eosin staining of femur tissues, osteogenesis, autophagy, and AMPK/mTOR signaling were analyzed. In vitro, MC3T3‐E1 cells were treated with 0, 1, 5, 10, 20, and 100 μmol/L Rg3. 10 and 20 μmol/L Rg3, which had no significant effect on cell viability and significantly affected AMPK/mTOR signaling, were chosen for further analysis. Then osteogenic differentiation was induced with Rg3 or/and AMPK inhibitor (Compound C). AMPK/mTOR signaling, autophagy, osteogenic differentiation, and mineralization by Alizarin Red staining were analyzed. The expression or activity of AMPK/mTOR signaling‐related proteins, autophagy markers, and osteogenesis markers was measured by western blotting or commercial kits, and cell viability by cell counting kit‐8 assay kits. Rg3 significantly alleviated OVX‐induced BW increases, BMD declines and histological changes of femur tissues, promoted osteogenesis, autophagy, and AMPK signaling, but inhibited mTOR signaling in vivo. Moreover, Rg3 significantly enhanced AMPK signaling, autophagy, osteogenic differentiation, and mineralization, but suppressed mTOR signaling in vitro. However, Compound C significantly reversed Rg3‐induced alterations in vitro, indicating that Rg3 regulated autophagy, osteogenic differentiation, and mineralization via AMPK/mTOR signaling. Hence, it was speculated that Rg3 might attenuate OVX‐induced osteoporosis via AMPK/mTOR signaling pathway.

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Protective Effect of Acteoside on Ovariectomy-Induced Bone Loss in Mice

Cited by 30 publications

References 31 publications

Cistanches Herba, from an endangered species to a big brand of Chinese medicine

Cistanches Herba, from an endangered species to a big brand of Chinese medicine

A network pharmacology approach to explore and validate the potential targets underlying the effect of Ginsenoside on Osteoporosis

Ginsenoside Rg3 attenuates ovariectomy‐induced osteoporosis via AMPK/mTOR signaling pathway

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