Protective effect of a naked DNA vaccine cocktail against lethal toxoplasmosis in mice

DNA Immunization with Na + -K + ATPase ( Sseat-6 ) Induces Protective Immunity to Larval Strongyloides stercoralis in Mice

“…In contrast, the peptide-specific IgG response to ATP1 and ATP2 peptides was almost completely eliminated when mice were immunized with all three constructs, but it still remained significantly higher than that of the control group. Cocktail vaccines often show enhanced protective effects compared to those of single antigens and genes (15,58). However, in other studies it has been observed that immunization with a cocktail vaccine gave no better protection than that seen with single antigens, and in fact the antibody responses to individual antigens was reduced after immunization with multiple antigens simultaneously (1).…”

GRA1 protein vaccine confers better immune response compared to codon-optimized GRA1 DNA vaccine

“…Other studies using ROP2 or SAG1 single gene DNA vaccinations did not increase survival significantly [12,18]; however, ROP2-SAG1 cocktail vaccine and DNA vaccine encoding T. gondii genomic library increased survival up to four times compared to controls [12,13]. Overall, the elicited immune response, as determined by IgG1, IgG2a, IFN-␥ and IL-4 levels, as well as the protection potency was somewhat higher for the CMV/co-GRA1 DNA vaccine than for the CMV/wt-GRA1 DNA vaccine and mammalian codon-optimized GRA1 DNA vaccine showed immunogenicity and protection comparable to previous vaccination studies.…”

“…RH strain tachyzoites were maintained in confluent monolayers of human foreskin fibroblasts (HFF) cell culture grown in Dulbeccos modified Eagle medium (DMEM) supplemented with 10% (v/v) heat inactivated fetal bovine serum (Hyclone, Logan, UT), 2 mM l-glutamine and 50 mg gentamycin per ml (Invitrogen-Gibco, Carlsbad, CA). T. gondii antigen (TAg) was prepared with the RH strain tachyzoites obtained from HFF cell culture as described [12].…”

Vaccines against Toxoplasma gondii: challenges and opportunities

“…Prolonged protection against acute toxoplasmosis upon infection with T. gondii RH in BALB/c mice was reported for a cocktail DNA vaccine combining SAG1 with ROP2 (Fachado et al 2003a), but not for the single gene DNA vaccines. Similarly, enhanced protection was observed in mice after immunization with a plasmid based combination of GRA4 and SAG1.…”