Protective effect of 3-O-methyl quercetin and kaempferol from Semecarpus anacardium against H2O2 induced cytotoxicity in lung and liver cells

Abstract

BackgroundHydrogen peroxide is continuously generated in living cells through metabolic pathways and serves as a source of reactive oxygen species. Beyond the threshold level, it damages cells and causes several human disorders, including cancer.MethodsEffect of isolated 3-O-methyl quercetin and kaempferol on H2O2 induced cytotoxicity, ROS formation, plasma membrane damage, loss of mitochondrial membrane potential, DNA damage was evaluated in normal liver and lung cells. The RT-PCR analysis used to determine Nrf 2 gene expression. Calorimetric ELISA was used to determine Nrf2 and p-38 levels. Expression of SOD and catalase was analyzed by Western blot analysis.ResultsThe present study isolated 3-O-methyl quercetin and kaempferol from the stem bark. They protected normal lung and liver cells from H2O2 induced cytotoxicity, ROS formation, membrane damage and DNA damage. Pre-treatment with 3-O-methyl quercetin and kaempferol caused translocation of Nrf2 from cytosol to nucleus. It also increased expression of p-p38, Nrf2, SOD and catalase in H2O2 treated lung and liver cells.ConclusionThe flavonoids isolated from S. anacardium significantly reduced H2O2 induced stress and increased expression of Nrf2, catalase and superoxide dismutase-2 indicating cytoprotective nature of 3-O-methylquercetin and kaempferol.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1354-z) contains supplementary material, which is available to authorized users.

Ameliorative Effects of Kaempferol and Zinc Gluconate on Erythrocyte Osmotic Fragility and Haematological Parameters in Wistar Rats Exposed to Noise Stress

“…The PCV, Hb concentration and total erythrocyte count were decreased. The findings from this study are in agreement with the results obtained by Kumar et al [49] and Minka and Ayo [50] who reported. That the decrease observed in the PCV, Hb and total erythrocyte count in the stressed rats may be due to increased lipid peroxidation in erythrocyte cell membranes and subsequent haemoglobin oxidation [50].…”

Kaempferol alleviates the reduction of developmental competence during aging of porcine oocytes

“…KAE has been reported to upregulate Nrf2-mediated HO-1 expression (Hong et al, 2009) Kim, Ha, et al, 2008;Kumar, Bevara, Kaja, Badana, & Malla, 2016;Martin et al, 2004;Samhan-Arias, Martıń-Romero, & Gutiérrez-Merino, 2004). These effects may also have been important for the significant reduction in ROS levels induced by KAE in the aged oocyte group in this study.…”

“…SOD, Nrf2 expression abnormalities and mitogen‐activated protein kinase (MAPK), phosphatidylinositol 3‐kinase (PI3K) signaling pathway disorder are key pathways for generating oxidative stress/damage (Ryter et al, ; Singh, Vrishni, Singh, Rahman, & Kakkar, ). KAE has been reported to upregulate Nrf2‐mediated HO‐1 expression (Hong et al, ), and relieve H 2 O 2 ‐induced oxidative stress by increasing the levels of p‐p38, Nrf2, SOD, and catalase (Kim, Lee, et al, ; Kim, Ha, et al, ; Kumar, Bevara, Kaja, Badana, & Malla, ; Martin et al, ; Samhan‐Arias, Martı́n‐Romero, & Gutiérrez‐Merino, ). These effects may also have been important for the significant reduction in ROS levels induced by KAE in the aged oocyte group in this study.…”

Virtual screening of MAP-Tauprotein inhibitors fromSemecarpus anacardiumLinn. leaf extract for cancer prevention

“…It have been reported by scientific study recently to posse various properties like hypoglycemic, antiatherogenic, antimicrobial, antioxidant, anticarcinogenic, antiinflammatory, anti-reproductive, CNS stimulant, skin diseases and hair growth promoter [28][29][30][31][32][33]. It shows in vitro antioxidant properties and induces cytotoxicity in liver, lung [34,35], breast, leukaemia, cervical cancer cells [36,37] through induction of apoptosis [37,38]. It also has antitumor activity in tumor bearing rat [39,40] and mice model [41].…”