Protective Activity of S-PT84, a Heat-killed Preparation of Lactobacillus pentosus, against Oral and Gastric Candidiasis in an Experimental Murine Model

Hayama, Koremasa; Ishijima, Sanae A.; Ono, Yuichi; Izumo, Takayuki; Ida, Masayuki; Shibata, Hiroshi; Abe, Shigeru

doi:10.3314/mmj.55.j123

Cited by 11 publications

(8 citation statements)

References 13 publications

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“…Recently, certain probiotic bacteria have been studied as a potential method to prevent opportunistic infectious diseases by stimulating the host immune system (7–9). Additionally, previous studies have reported the positive effects of probiotics in mucosal candidiasis, such as Candida vaginitis and vulvovaginal candidiasis, oral candidiasis, and gastrointestinal infection (10–13). To date, however, very little research has been done on the effect of probiotics in the setting of CD.…”

Section: Introductionmentioning

confidence: 99%

Effects of a Novel Probiotic Combination on Pathogenic Bacterial-Fungal Polymicrobial Biofilms

Hager

Isham

Schrom

et al. 2019

mBio

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Dysbiosis of the gut microbiome has been implicated in inflammatory bowel diseases. We have shown that levels ofCandida tropicalis, along with those ofEscherichia coliandSerratia marcescens, are significantly elevated in Crohn’s disease (CD) patients. Here, we evaluated the ability of a novel probiotic to prevent and treat polymicrobial biofilms (PMB) formed byC. tropicaliswithE. coliandS. marcescens. SinceCandida albicanshas been reported to be elevated in CD patients, we investigated the interactions ofC. albicanswith these bacterial species in biofilm formation. We determined whether the interaction betweenCandidaspp. and bacteria is specific by usingTrichosporon inkinandSaccharomyces fibuligeraas comparators. Additionally, the effects of probiotics onC. albicansgermination and biofilm formation were determined. To determine the ability of the probiotic to prevent or treat mature biofilms, probiotic filtrate was added to the PMB at early (prevention) and mature (treatment) phases. Biofilm thickness and architecture were assessed by confocal scanning laser microscopy. The effects of the probiotic on germination were evaluated in the presence of serum. Exposure ofC. tropicalisPMB to probiotic filtrate reduced biofilm matrix, decreased thickness, and inhibited hyphal formation. We showed thatC. albicansorC. tropicalisformed significantly thicker PMB than control biofilms, indicating that this interaction isCandidaspecific. Treatment with probiotic filtrate inhibitedC. albicansgermination and prevented/treatedC. albicansPMB. The designed probiotic may have utility in the management of biofilm-associated gastrointestinal diseases such as Crohn’s and colorectal cancer.IMPORTANCEThe effects of diversity of the gut microbiome on inflammation have centered mainly on bacterial flora. Recent research has implicated fungal species and their interactions with other organisms in the inflammatory process. New ways to restore microbial balance in the gut are being explored. Our goal was to identify beneficial probiotic strains that would antagonize these fungal and bacterial pathogens that are elevated in the inflamed gut, and which also have antibiofilm activity. Fungus-bacterium correlation analysis allowed us to identify candidate probiotic species that can antagonize microbial pathogens, which we subsequently incorporated into a novel probiotic formulation. Amylase, which is known to have some antibiofilm activity, was also added to the probiotic mixture. This novel probiotic may have utility for the management of inflammatory bowel diseases by disrupting polymicrobial biofilm formation.

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Section: Introductionmentioning

confidence: 99%

Effects of a Novel Probiotic Combination on Pathogenic Bacterial-Fungal Polymicrobial Biofilms

Hager

Isham

Schrom

et al. 2019

mBio

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“…Izumo et al (28,29) and Hayama et al (30) reported that the Lactobacillus (L.) pentosus strain S-PT84, isolated from Kyoto pickles, has inhibitory activity against influenza virus, Salmonella Typhimurium, and Candida albicans infection in mice, and S-PT84 enhances IFN-α production from plasmacytoid dendritic cells by virus stimulation (31).…”

Section: Introductionmentioning

confidence: 99%

Sulforaphane enhances apoptosis induced by Lactobacillus pentosus strain S‑PT84 via the TNFα pathway in human colon cancer cells

2019

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Sulforaphane and Lactobacilli induce apoptosis in several cancer cells. Sulforaphane, a dietary isothiocyanate, is an attractive agent due to its potent anticancer effects. Sulforaphane suppresses the proliferation of various cancer cells in vitro and in vivo. The present study investigated the effect of sulforaphane and a co-culture with Lactobacillus-treated peripheral blood mononuclear cells (PBMCs) in human colon cancer cells. The combination markedly induced apoptosis in human colon cancer HCT116 and SW480 cells. A pan-caspase inhibitor markedly inhibited apoptosis, and a tumor necrosis factor (TNF) receptor/Fc chimera partially inhibited apoptosis in both cells. The amount of TNFα secretion in the culture supernatant was significantly increased by co-culture with Lactobacillus-treated normal human PBMCs. On the other hand, the expression of cellular inhibitor of apoptosis-2 (cIAP-2), an anti-apoptotic protein, was increased by co-culture with Lactobacillus-treated PBMCs in colon cancer cells, but sulforaphane treatment significantly suppressed the induction of cIAP-2. The present results revealed that sulforaphane enhances apoptosis in human colon cancer cells under co-culture with Lactobacillus-treated PBMCs via the TNFα signaling pathway.Abbreviations: PBMCs, peripheral blood mononuclear cells; TNF, tumor necrosis factor

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“…Certain probiotic bacteria have been evaluated for their potential to re-establish the microbial balance and thus prevent or reverse the adverse effects of such diseases as vaginal and oral yeast infections and GI tract infections [7][8][9][10][11]. In previous studies, we were able to identify probiotic species of bacteria and fungi that are able to support the beneficial GI microbes while simultaneously antagonizing pathogenic ones [12].…”

Section: Introductionmentioning

confidence: 99%

BIOHM Probiotics Retain Viability in Low pH Environments Simulating the Digestive Environment

Ghannoum¹,

Ghannoum²,

Long³

et al. 2019

J Prob Health

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Probiotics in various combinations and in various forms have become popular supplements for establishing and maintaining gut health. However, commercial products vary in the effectiveness of their specific probiotic strains and the ability of these strains to survive the acidic environment of the stomach. A novel probiotic, manufactured by BIOHM, LLC, has a unique formulation of Saccharomyces boulardii, Lactobacillus acidophilus, L. rhamnosus, and Bifidobacterium breve, in combination with amylase, which has been developed to re-balance the bacterial and fungal population of the human gastrointestinal tract and combat digestive biofilms formed by pathogenic bacteria and fungi. Our data shows that these strains have the ability to survive the acidic environment when taken within 30 minutes of meal, a factor that is vital to ensure probiotic effectiveness.

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Protective Activity of S-PT84, a Heat-killed Preparation of Lactobacillus pentosus, against Oral and Gastric Candidiasis in an Experimental Murine Model

Cited by 11 publications

References 13 publications

Effects of a Novel Probiotic Combination on Pathogenic Bacterial-Fungal Polymicrobial Biofilms

Effects of a Novel Probiotic Combination on Pathogenic Bacterial-Fungal Polymicrobial Biofilms

Sulforaphane enhances apoptosis induced by Lactobacillus pentosus strain S‑PT84 via the TNFα pathway in human colon cancer cells

BIOHM Probiotics Retain Viability in Low pH Environments Simulating the Digestive Environment

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