Protective activity of melanin-like pigment derived from tea on Drosophila melasnogaster against the toxic effects of benzidine

Sava, Vasyl; Hung, Yao-Ching; Галкин, Б. Н.; Hong, Meng-Yen; Huang, G. S.

doi:10.1016/s0963-9969(01)00163-6

Cited by 15 publications

(9 citation statements)

References 28 publications

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“…37) RTM behaves as a chain-breaking antioxidant that prevents the propagation of free radicals through reactions between the phenol groups and the peroxide radicals. NRTM is not capable of terminating free radical chain reactions because of its lack of phenol groups, although its quinone groups can decrease the oxidation rate slightly 25) through their reactions with alkyl free radicals.…”

Section: Antioxidant Activity Of Tea Melaninmentioning

confidence: 99%

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Protective Effects of Tea Melanin against 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Induced Toxicity: Antioxidant Activity and Aryl Hydrocarbon Receptor Suppressive Effect

Hung

Huang

Sava

et al. 2006

Biological & Pharmaceutical Bulletin

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We examined the protective ability of tea melanin against 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced toxicity in C57BL6J mice. Reduced tea melanin (RTM) and non-reduced tea melanin (NRTM) were incorporated to distinguish anti-oxidant activity from alternative pathways. The mice were given a single oral dose of TCDD (100 m mg/kg body weight) and then they were administered daily with NRTM or RTM (40 mg/kg, p.o.) for next 14 d. RTM protected the animals against TCDD-induced lipid peroxidation, inhibition of glutathione peroxidase, alteration in reduced and oxidized glutathione concentrations, loss of body weight, and increased relative liver weight. NRTM was less effective as compared to RTM because of its inferior antioxidant activity, but it still displayed a strong protective effect against TCDD toxicity owing to its similar suppression of the activity of the aryl hydrocarbon receptor. Both NRTM and RTM suppressed the expression of CYP1A1 gene and prevented the activation of cytochrome P450 isozyme in the livers of animals exposed to TCDD. These results suggest that tea melanin might be a potential agent offering dual protection against the development of TCDD-induced oxidative stress.

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Section: Antioxidant Activity Of Tea Melaninmentioning

confidence: 99%

“…[20][21][22] Tea melanin also exhibits a protective activity against a number of toxic substances, such as benzidine, hydrazine, and snake venoms. [23][24][25][26] The current study was undertaken based on the multiple bioactivities of tea melanin. We employed preparations of a reduced form of tea melanin (RTM) and a non-reduced tea melanin (NRTM).…”

mentioning

confidence: 99%

Protective Effects of Tea Melanin against 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Induced Toxicity: Antioxidant Activity and Aryl Hydrocarbon Receptor Suppressive Effect

Hung

Huang

Sava

et al. 2006

Biological & Pharmaceutical Bulletin

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“…One severely deleterious agent is hydrazine. It is a mutagenic compound that is responsible for the hepatotoxicity of the anti-tubercular medicine isoniazid and increases risks of systemic diseases and cancer 5,31 . Thus in the present study, melanin from apricot kernels (MAKS) was isolated, and its physicochemical properties were examined.…”

Section: Introductionmentioning

confidence: 99%

Characterization of melanin extracted from apricot (Armeniaca sibirica) and its effect on hydrazine-induced rat hepatic injury

Li¹,

Li²,

Zhao³

2016

ScienceAsia

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ABSTRACT:Melanin is a complex, highly coloured natural antioxidant with potent biological activities and diverse applications. A potential source of melanin is the Siberian apricot (Armeniaca sibirica) whose kernel skin is currently a waste by-product. To explore its potential, melanin from apricot kernel skin was extracted. The molecular mass was determined by gel chromatography and its structure was examined by elemental analysis, UV-Vis, infrared, and solid-state nuclear magnetic resonance ( 13 C-NMR) spectroscopy. In addition, its protective effect against hydrazineinduced hepatic injury in rats was evaluated. The results showed that melanin has < 5% sulphur (S) content, indicating that it consists mostly of eumelanin. Its UV-Vis, infrared and 13 C-NMR spectra were very similar to those of synthetic melanin, although it had relatively high carboxylate, aliphatic, and phenol-skeleton content. Two distinct fractions (2.4 ± 0.12 and 0. ± 560.1 kDa) were detected. Injections of hydrazine at 65 mg/kg body-weight (b.wt.) doses resulted in significant (p < 0.01) increases in lipid peroxidation (malondialdehyde contents) in liver homogenates and serum alanine aminotransferase and aspartate aminotransferase activities (64%, 69%, and 98% increase relative to the controls, respectively). It also induced significant (p < 0.05 or 0.01) decreases in liver homogenate catalase, glutathione peroxidase, and superoxide dismutase activities and reduced glutathione content (48%, 28%, 7%, and 98% reduction, respectively). Administering melanin prior to hydrazine, at 10 mg/kg b.wt., strongly inhibited these changes and ameliorated histopathological hepatic injury. The results indicated that melanin alleviated hydrazine-induced liver injury by relieving oxidative stress, which may provide a novel protective strategy for protection against liver diseases.

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“…Melanin extracted from black tea (MEBT) represents the high molecular part of tea polyphenols (Sava et al, 2001b) with physico-chemical characteristics that are similar to those of typical melanin. However, MEBT revealed unexpected protective activity against various toxic substances (Sava et al, 2002;Sava et al, 2003;Hung et al, 2003).…”

Section: Introductionmentioning

confidence: 97%

Inhibitory effects on phospholipase A2 and antivenin activity of melanin extracted from Thea sinensis Linn

et al. 2004

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Protective activity of melanin-like pigment derived from tea on Drosophila melasnogaster against the toxic effects of benzidine

Cited by 15 publications

References 28 publications

Protective Effects of Tea Melanin against 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Induced Toxicity: Antioxidant Activity and Aryl Hydrocarbon Receptor Suppressive Effect

Protective Effects of Tea Melanin against 2,3,7,8-Tetrachlorodibenzo-p-dioxin-Induced Toxicity: Antioxidant Activity and Aryl Hydrocarbon Receptor Suppressive Effect

Characterization of melanin extracted from apricot (Armeniaca sibirica) and its effect on hydrazine-induced rat hepatic injury

Inhibitory effects on phospholipase A2 and antivenin activity of melanin extracted from Thea sinensis Linn

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