Protective activities of serum immunoglobulin G on the mucosal surface to Vibrio cholerae O1

Bougoudogo, F; Vély, Frederic; Nato, Farida; Boutonnier, A; Gounon, Pierre; Mazié, J. C.; Fournier, Jean‐Michel

doi:10.1016/0020-2452(96)85762-9

Cited by 21 publications

(19 citation statements)

References 27 publications

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“…Previous vaccine trials in Asia have shown that killed-cell Inaba vaccines protect against both serotypes, but Ogawa vaccines protect against only the homologous serotype (1). Similar results observed with serotype-specific mAbs tested for protective activity in neonatal mice (4)(5)(6) led to the conclusion that the Ogawa serotype contains a specific antigenic determinant, whereas the Inaba serotype contains a different antigenic determinant that crossreacts with the Ogawa serotype. The Ogawa serotype-specific epitope is probably associated with the upstream terminal perosamine residue of the O-SP, because Inaba strains are rfbT mutants of wild-type Ogawa strains in which methylation of the terminal perosamine is inactivated (2,3,7).…”

supporting

confidence: 62%

“…Indeed, the S-20-4 Ab shows no detectable binding to the synthetic terminal mono-and oligosaccharides of the Inaba O-SP lacking the 2-O-methyl group (8). Also, the binding of IgG1 S-20-3 (same clone as S-20-4) to Ogawa LPS could not be inhibited by the Inaba O-SP (6). The central pocket is primarily defined by residues Tyr L32, Trp L91, and Trp L96 from the light chain and residues His H95 and Ala H98 from the heavy (Fig.…”

Section: Resultsmentioning

confidence: 96%

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Crystal structure of an anti-carbohydrate antibody directed againstVibrio choleraeO1 in complex with antigen: Molecular basis for serotype specificity

Villeneuve

Souchon

Riottot

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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105

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The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is shown to be the primary antigenic determinant. Additional perosamine residues protrude outwards from the Ab surface and contribute only marginally to the binding affinity and specificity. A complementary water-excluding hydrophobic interface and five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine.

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supporting

confidence: 62%

Section: Resultsmentioning

confidence: 96%

Crystal structure of an anti-carbohydrate antibody directed againstVibrio choleraeO1 in complex with antigen: Molecular basis for serotype specificity

Villeneuve

Souchon

Riottot

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

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105

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“…In our effort to develop a conjugate vaccine that targets V. cholerae O1 and O139, we have developed monoclonal antibodies specific to V. cholerae O1 or O139 LPS (4,5). Here we have exploited the specificity of the monoclonal antibodies to develop rapid diagnostic tests for cholera O1 or O139 using colloidal gold particles and based on a recently optimized (7) one-step, vertical-flow immunochromatography principle (13).…”

mentioning

confidence: 99%

One-Step Immunochromatographic Dipstick Tests for Rapid Detection of Vibrio cholerae O1 and O139 in Stool Samples

Nato

Boutonnier

Rajerison

et al. 2003

Clin Vaccine Immunol

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We describe the development and evaluation of a rapid diagnostic test for Vibrio cholerae O1 and O139 based on lipopolysaccharide detection using gold particles. The specificity ranged between 84 and 100%. The sensitivity of the dipsticks ranged from 94.2 to 100% when evaluated with stool samples obtained in Madagascar and Bangladesh. The dipstick can provide a simple tool for epidemiological surveys.Vibrio cholerae strains belonging to the O1 and O139 serogroups are capable of causing epidemic and pandemic cholera. The O1 serogroup is subdivided into two serotypes, Ogawa and Inaba. Serogroup O139, which appeared in India in 1992, has spread rapidly throughout Asian countries and is considered to be the potential eighth pandemic strain of cholera. Prompt diagnosis of cholera is of key importance to initiate effective therapy and to institute proper epidemiological measures. There are definitive indications that the incidence of this serogroup is on the rise in India and Bangladesh (17).Several rapid diagnostic tests for cholera have been described. Some detect the cholera toxin (2, 19). The others detect the lipopolysaccharide (LPS) antigen of V. cholerae O1 (3, 6, 8, 12, 16) or O139 (1, 10, 14). Recently, a multistep colloidalgold-based colorimetric immunoassay known as SMART was also developed for direct detection of V. cholerae O1 (9, 11) or V. cholerae O139 (15) in stool specimens and has demonstrated 95% sensitivity and 100% specificity for O1 strains (11) and 100% sensitivity and 97% specificity for O139 strains (15).In our effort to develop a conjugate vaccine that targets V. cholerae O1 and O139, we have developed monoclonal antibodies specific to V. cholerae O1 or O139 LPS (4, 5). Here we have exploited the specificity of the monoclonal antibodies to develop rapid diagnostic tests for cholera O1 or O139 using colloidal gold particles and based on a recently optimized (7) one-step, vertical-flow immunochromatography principle (13). The detection threshold with purified LPS was 10 ng/ml for V. cholerae O1 and 50 ng/ml for V. cholerae O139. The dipsticks were stable after storage for 21 days at 60, 4, Ϫ20, and Ϫ80°C.We have evaluated the sensitivity and specificity of the rapid dipstick tests in the laboratory setting and in two areas of cholera endemicity, namely, in Madagascar and in Bangladesh. The specificity was assessed using 14 pure cultures of V. cholerae non-O1/non-O139 and 16 strains belonging to six other species of the genus Vibrio (V. alginolyticus, V. fluvialis, V. parahaemolyticus, V. furnissii, V. hollisae, and V. mimicus), seven strains of Aeromonas species (A. caviae, A. enteropelogenes, A. hydrophila, A. sobria, and A. trota), two strains of Plesiomonas shigelloides, and two strains of Campylobacter jejuni. Additionally, eight strains of Yersinia pseudotuberculosis, 10 strains of Yersinia enterocolitica, 35 other strains of Yersinia belonging to seven species, and another 47 strains belonging to 11 other genera of Enterobacteriaceae were included. The specificity of both dipsticks was ...

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“…PBS, pH 7.3 is a mixture of 0.05 M potassium phosphate buffer and 0.15 M NaCl. Mouse anti-LPS monoclonal Abs (mAbs), IgG I-24-2, F-22-30, and S-20-3 were prepared at the Institut Pasteur by immunizing mice with a lysate from V. cholerae O1 serotype Inaba [31][32][33]. Analytical and semi-preparative RP-HPLC separations were performed using a PerkinElmer Series 200 pump and a 785A UV/VIS detector system on a Kromasil C18 (A. I. T. chromato, France) (100 Å, 4.6×250 mm), column at a flow rate of 1 mL min −1 (monitoring and analysis), or on a Nucleosil 100-5 C18 (Macherey-Nagel) (100 Å, 5 μm, 10×250 mm) column, at a flow rate of 3 mL min −1 (semi-preparative), with detection at 215 nm.…”

Section: Methodsmentioning

confidence: 99%

Investigation towards bivalent chemically defined glycoconjugate immunogens prepared from acid-detoxified lipopolysaccharide of Vibrio cholerae O1, serotype Inaba

et al. 2008

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A free amino group present on the acid-detoxified lipopolysaccharide (pmLPS) of V. cholerae O1 serotype Inaba was investigated for site-specific conjugation. Chemoselective pmLPS biotinylation afforded the corresponding mono-functionalized derivative, which retained antigenicity. Thus, pmLPS was bound to carrier proteins using thioether conjugation chemistry. Induction of an anti-LPS antibody (Ab) response in BALB/c mice was observed for all conjugates. Interestingly, the sera had vibriocidal activity against both Ogawa and Inaba strains opening the way to a possible bivalent vaccine. However, the level of this Ab response was strongly affected by both the nature of the linker and of the carrier. Furthermore, no switch from IgM to IgG, i.e. from a T cell-independent to a T cell-dependent immune response was detected, a result tentatively explained by the possible presence of free polysaccharide in the formulation. Taken together, these results encourage further investigation towards the development of potent pmLPS-based neoglycoconjugate immunogens, fully aware of the challenge faced in the development of a cholera vaccine that will provide efficient serogroup coverage.

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Protective activities of serum immunoglobulin G on the mucosal surface to Vibrio cholerae O1

Cited by 21 publications

References 27 publications

Crystal structure of an anti-carbohydrate antibody directed againstVibrio choleraeO1 in complex with antigen: Molecular basis for serotype specificity

Crystal structure of an anti-carbohydrate antibody directed againstVibrio choleraeO1 in complex with antigen: Molecular basis for serotype specificity

One-Step Immunochromatographic Dipstick Tests for Rapid Detection of Vibrio cholerae O1 and O139 in Stool Samples

Investigation towards bivalent chemically defined glycoconjugate immunogens prepared from acid-detoxified lipopolysaccharide of Vibrio cholerae O1, serotype Inaba

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