Protective action of hydroxyethyl rutosides on singlet oxygen-challenged cardiomyocytes

Olbrich, H. G.; Grabisch, P.; Matheis, G.; Mutschler, E.

doi:10.1016/0967-2109(95)94323-o

Cited by 1 publication

(1 citation statement)

References 24 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The protective action occurred at comparatively low (nanomolar) concentrations and displayed a bell-shaped dose-response curve. This finding confirms a preliminary observation from our laboratory (Olbrich et al, 1994). Interestingly, HR caused an improvement of the number of elongated rod shaped cardiomyocytes during exposure to singlet oxygen without reducing the accumulation of TBARS.…”

Section: Discussionsupporting

confidence: 92%

Protective action of hydroxyethyl rutosides on singlet oxygen challenged cardiomyocytes

Olbrich

Grabisch

Großmann

et al. 1996

British J Pharmacology

View full text Add to dashboard Cite

The effect of a standardized mixture of β‐hydroxyethyl rutosides against oxidative damage in singlet oxygen‐challenged isolated cardiac myocytes from adult rats was investigated. The morphology of the myocytes was evaluated as an indicator for cell viability (elongated, rod shaped cells vs. hypercontracted, rounded cells). The determination of the production of thiobarbituric acid reactive substances served as an indicator for lipid peroxidation. Exposure to singlet oxygen which was generated by photo‐excitation of rose bengal (10−7 m) reduced the number of rod shaped (vital) cardiomyocytes by 78.5 ± 2.5% and increased the production of thiobarbituric acid reactive substances by 1180 ± 150% in comparison to incubation with control buffer. Coincubation of the cells with β‐hydroxyethyl rutosides (concentration range: 6.7 pg ml−1 to 670 μg ml−1) increased the number of rod shaped cardiomyocytes after exposure to singlet oxygen in a dose‐dependent bell‐shaped manner. A significant protective effect was observed at β‐hydroxyethyl rutosides concentrations ranging from 0.67 ng ml−1 to 67 ng ml−1. In spite of their protective action, β‐hydroxyethyl rutosides did not reduce the accumulation of thiobarbituric acid reactive substances, used as an indicator for lipid peroxidation. The data suggest that β‐hydroxyethyl rutosides exert a protective action against oxygen radical‐induced damage of cardiac myocytes at very low concentrations without interfering with lipid peroxidation.

show abstract

Section: Discussionsupporting

confidence: 92%