Protection of Shuxuetong combined with aspirin against cerebral ischemia/reperfusion injury through ameliorating coagulation and fibrinolysis system

Wu, Yongmei; Zhai, Yadong; Zhou, Yuting; Tang, Shimin; Li, Xin-yue; Jia, Lifu; Meng, Xiangbao; Zhang, Hui; Sun, Guibo

doi:10.1016/j.chmed.2018.03.011

Cited by 2 publications

(1 citation statement)

References 14 publications

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“…It has the effect of promoting blood circulation and removing blood stasis. Clinically, it is used in the acute phase of meridian in ischemic stroke caused by congestion, with syndromes containing hemiparalysis, deviation of eyes and mouth, language spasm, and acute cerebral infarction [1][2] . At present, the studies on SXT injection are mainly focused on evaluation and observation of pharmacological activity and clinical effects, and their results show that SXT has anticoagulation, thrombolytic, lipid regulation, and cell protection effects [3][4][5] .…”

Section: Introductionmentioning

confidence: 99%

The integrated analysis strategy of unstable hypoxanthine, a potential quality marker in Shuxuetong injection based on standard addition method and multi-level pharmacokinetics by LC-MS/MS

Xing

Wang

et al. 2023

Acupuncture and Herbal Medicine

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Objective: As an injection made from traditional Chinese medicine, Shuxuetong (SXT) injection is used for the treatment of ischemic stroke. Hypoxanthine is regarded as one of its potential quality markers. The purpose of this study is to lay the foundation for the quality control of SXT injection by the analysis of the quantitation and pharmacokinetic behavior of hypoxanthine. Methods: A quantitative method of hypoxanthine in SXT injection based on standard addition method by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was established for the first time. On the other hand, a determination method of hypoxanthine in rat plasma samples after administration of SXT was also successfully established based on LC-MS/MS. Results: It was found that the content of hypoxanthine was higher using conventional liquid-mass spectrometry technology compared to the application of LC-MS/MS combined standard addition method in the same batch of SXT injection. The ratio of low, medium and high doses of intravenous SXT were 1:2:4, and the AUC0-t was (848.34 ± 324.53) μg·h/L, (1483.94 ± 497.74) μg·h/L, and (3074.84 ± 910.29) μg·h/L, respectively. AUC0-t shows a good linear dose-dependent relationship. Conclusions: The influences of endogenous substances tend to be eliminated by calibrating the concentration level of the target compound by the introduction of the standard addition method. The added allopurinol could inhibit the conversion of the target compound, and ensure the accuracy of the detection during the pharmacokinetic studies. “Blank biological matrix” obtained from the pretreatment of blank plasma successfully distinguished endogenous and drug-derived hypoxanthine. There is a good linear relationship between the blood concentration of intravenous hypoxanthine and the dosage of administration. Similarly, there was no drug accumulation in the multiple medium-dosage group, which is similar to the pharmacokinetic characteristics of the single medium-dosage group. Graphical abstract: http://links.lww.com/AHM/A59

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