Protection of piglets against enteric colibacillosis by intranasal immunization with K88ac (F4ac) fimbriae and heat labile enterotoxin of Escherichia coli

Immunogenicity of recombinantLactobacillus casei-expressing F4 (K88) fimbrial adhesin FaeG in conjunction with a heat-labile enterotoxin A (LTAK63) and heat-labile enterotoxin B (LTB) of enterotoxigenicEscherichia colias an oral adjuvant in mice

“…Our results indicate that LT can enhance the immune response induced by F4 fimbriae and enhance protection against ETEC challenge (Lin et al . ). Moreover, our data demonstrate that non‐toxic LTA mutant LTAK63 retained its adjuvant properties and eliminated the toxicity associated with the native toxin, which agrees with that of a previous report (Sjökvist Ottsjö et al .…”

“…; Lin et al . ), the protective effect observed in the vaccine/adjuvant co‐administration groups might because LTAK63 and LTB acted not only as mucosal adjuvants that enhanced the FaeG‐specific antibody response but also as antigens that could directly prime an immune response against ETEC. Thus, oral immunization with genetically engineered L. casei ‐expressing FaeG coupled with rLpPG‐2‐LTAK63‐co‐LTB or rLpPG‐2‐LTAK63‐fu‐LTB as an adjuvant is a novel approach to protection against F4+ ETEC infection.…”

Virulence factors of Escherichia coli in relation to the importance of vaccination in pigs

“…Other methods to reduce losses due to ETEC are active immunization (intramuscular immunization with fimbriae or oral immunization with attenuated or non-virulent strains which contain fimbriae), passive immunization (oral administration of yolk immunoglobulin from eggs (IgY) against fimbriae), use of powdered plasma proteins (FAIRBROTHER et al, 2005), and intranasal vaccines in piglets to prevent diarrhea (LIN et al, 2013). Measures of control and prevention are listed in table 2.…”

Identification and quantification of virulence factors of enterotoxigenic Escherichia coli by high-resolution melting curve quantitative PCR

“…Successful immunisation strategies target the different fimbriae addition to the toxins, and hence require knowledge on the fimbriae that are related to disease development [47]. Oral immunization with recombinant vaccines such as recombinant K88/LT vaccine [22] or recombinant K88/K99/F6/F41/F18 fimbriae proteins [15] was more advantageous in stimulation of systematic and mucosal immunity [22]. Strategies that employ receptor decoys to prevent binding of specific fimbriae types to the glycan receptor on the surface of the intestinal mucosa provide an alternative therapeutic option to prevent ETEC-induced diarrhea in young pigs.…”

“…The profile of virulence genes in swine isolates of ETEC varies with the age of the animals and the geographical location; ETEC carrying K88 fimbriae are more frequent in neonate animals while ETEC carrying F18 fimbriae are more frequent in weanling pigs [8–10, 19, 21]. Moreover, vaccination of piglets with a recombinant K88/LT vaccine provided superior protection against ETEC K88 challenge when compared to vaccine with K88 or LT antigens alone [22]. The diversity of virulence factors of ETEC and role of fimbriae as targets for therapeutic intervention highlight the need of effective methods that differentiate fimbriae of ETEC [18, 23–25].…”