Protection of Penaeus monodon against white spot syndrome virus using a WSSV subunit vaccine

Witteveldt, Jeroen; Vlak, Just M.; Hulten, M.C.W. van

doi:10.1016/j.fsi.2003.09.006

Cited by 198 publications

(156 citation statements)

References 23 publications

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“…The envelope protein VP28 was chosen as a vaccine candidate in this study because of its credibility to provide better survival upon WSSV infection [7,9]. Moreover, VP28 is more stable in the digestive environment than VP19 or better escapes from digestion.…”

Section: Wssv-vaccines and Immunohistochemistrymentioning

confidence: 99%

Truncated VP28 as oral vaccine candidate against WSSV infection in shrimp: An uptake and processing study in the midgut of Penaeus monodon

Kulkarni

Rombout

Singh

et al. 2013

Fish & Shellfish Immunology

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a b s t r a c tSeveral oral vaccination studies have been undertaken to evoke a better protection against white spot syndrome virus (WSSV), a major shrimp pathogen. Formalin-inactivated virus and WSSV envelope protein VP28 were suggested as candidate vaccine components, but their uptake mechanism upon oral delivery was not elucidated. In this study the fate of these components and of live WSSV, orally intubated to black tiger shrimp (Penaeus monodon) was investigated by immunohistochemistry, employing antibodies specific for VP28 and haemocytes. The midgut has been identified as the most prominent site of WSSV uptake and processing. The truncated recombinant VP28 (rec-VP28), formalin-inactivated virus (IVP) and live WSSV follow an identical uptake route suggested as receptor-mediated endocytosis that starts with adherence of luminal antigens at the apical layers of gut epithelium. Processing of internalized antigens is performed in endo-lysosomal compartments leading to formation of supra-nuclear vacuoles. However, the majority of WSSV-antigens escape these compartments and are transported to the inter-cellular space via transcytosis. Accumulation of the transcytosed antigens in the connective tissue initiates aggregation and degranulation of haemocytes. Finally the antigens exiting the midgut seem to reach the haemolymph. The nearly identical uptake pattern of the different WSSV-antigens suggests that receptors on the apical membrane of shrimp enterocytes recognize rec-VP28 efficiently. Hence the truncated VP28 can be considered suitable for oral vaccination, when the digestion in the foregut can be bypassed.

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Section: Wssv-vaccines and Immunohistochemistrymentioning

confidence: 99%

Truncated VP28 as oral vaccine candidate against WSSV infection in shrimp: An uptake and processing study in the midgut of Penaeus monodon

Kulkarni

Rombout

Singh

et al. 2013

Fish & Shellfish Immunology

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“…Research conducted by Rodríguez et al (2003) suggested an apparent association between lower temperature and increased shrimp mortality rates. Thus, given importance of this industry and the limitations of high-intensity cultivation due to viral infections causing high mortality (Ramos-Carreño et al, 2014;Melena et al, 2015), particularly in extreme salinities (50), the development of prevention methods against WSV and other viruses would be necessary (Witteveldt et al, 2004a(Witteveldt et al, , 2004b). …”

Section: ______________________mentioning

confidence: 99%

“…Nevertheless, our results are in line with findings by Prior et al (2003) showing that no increase in the survival rate was given by injection of inactive virus, under these experimental conditions. Thus, further analysis should be conducted to elucidate whether 1) WSV amount which is available to enter the specimen's system differs among injection methods or specimens, 2) strain virulence might differ among isolation sites, favouring a higher/lower mortality rate, and 3) growth stage and shrimp species would play an important role by influencing the adaptation to stress stimuli and the prevention of further infections (Witteveldt et al, 2004a(Witteveldt et al, , 2004bVaseeharan et al, 2006;Melena et al, 2015;Chen et al, 2016).…”

Section: ______________________mentioning

confidence: 99%

Survival in juvenile shrimps (Penaeus vannamei) exposed to inactive against active white spot virus: a challenge bioassay perspective

Muñoz-Naranjo¹,

Gilbert‐Jaramillo²,

Marcillo-Gallino³

et al. 2018

lajar

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ABSTRACT. White spot syndrome virus (WSSV) has damaged the Ecuadorian shrimp culture industry severely. The shrimp industry is highly important as it has generated high revenues over the past few years in Ecuador. Therefore, research on preventing devastating viral infections such as WSSV, is of major relevance. This study aimed to evaluate the survival rate in white shrimps (Penaeus vannamei) by using a vaccination method of inactive white spot virus (WSV) against an active WSV infection. A bioassay using 120 shrimps, 60 injected with inactive WSV and 60 injected with shrimp tissue without WSV infection, was conducted. Firstly, 30 specimens injected with inactive WSV were challenged against active WSV after 48 h. The remaining 30 specimens were challenged against shrimp tissue without WSV infection, as a negative control. Secondly, 60 specimens injected with the tissue without WSV infection were challenged as follows: 30 against active WSV and 30 against shrimp tissue without WSV infection. Kaplan-Meier analysis (P  0.961) of the results showed no significant differences between the groups. Thus, these results showed no decrease in the mortality rate in juvenile shrimps (P. vannamei) after treatment.

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“…The immune inductions of shrimp through vaccination techniques have been successfully conducted using protein recombinant WSSV on P. chinensis (Kim et al, 2004) and antiviral dsRNA on L. vannamei (Robalino et al, 2004), and to stimulate the gonad maturation in tiger shrimp (Wulandari, 2010;Treerattrakool et al, 2013), to protect against infectious virus on L. vannamei (Loy et al, 2012;Solis-Lucero et al, 2016),P. japonicas (Namikoshi et al, 2004), and P. monodon (Witteveldt et al, 2004). The application of antisense RNA in protecting L. vannamei from WSSV infection was also reported by Akhila et al (2015).…”

Section: Characteristic Of a Viral Protein (Vp-15) Of White Spot Syndmentioning

confidence: 99%

CHARACTERISTICS OF VIRAL PROTEIN, VP-15, OF WHITESPOT SYNDROME VIRUS ISOLATED FROM INFECTED TIGER SHRIMP Penaeus monodon (Fabricius, 1798)

Parenrengi

Alimuddin

Tenriulo

2017

Indonesian Aquaculture Journal

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White spot syndrome virus (WSSV) has caused mass mortality on tiger shrimp (Penaeus monodon) culture and adversely affects prawn industry worldwide including Indonesia. It is well known that the protein structure of WSSV plays an important role in the virus infection and morphogenesis process. A viral protein structure called VP-15 is located in the nucleocapsid of virion virus. The protein structure involves in the life cycle of WSSV in host cells. A gene encoding VP-15 could be involved in constructing the RNA interference (RNAi), so it is needed to isolate and characterize for RNAi technology purpose. The study was aimed to isolate and characterize the VP-15 from the infected WSSV tiger shrimp. The characterization of VP-15 was undertaken through assessment of nucleotide sequence, amino acid deduction, alignment nucleotide/protein searches using Genetyx and BLAST program, and dendrogram construction analysis. The results showed that VP-15 was successfully isolated in form of ORFDNA with a fragment size of 243 bp. The phylogenetic tree analysis revealed three clusters corresponding to the time (year) of isolates collection. The VP-15 consisted of 80 amino acids, two start codons (ATG), one stop codon (TAA), and one Kozak context (AAAATGG). Hydrophilic amino acid was the highest composition (44.2%), followed by neutral (31.2%) and hydrophobic (24.6%) amino acid groups. The VP-15 was rich in amino acid of lysine (21.3%), arginine (22.9%) and serine (24.6%). The successful isolation of VP-15 is a very important step in providing a basic yet suitable material in constructing the dsRNA vaccine to control shrimp diseases in aquaculture.

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Protection of Penaeus monodon against white spot syndrome virus using a WSSV subunit vaccine

Cited by 198 publications

References 23 publications

Truncated VP28 as oral vaccine candidate against WSSV infection in shrimp: An uptake and processing study in the midgut of Penaeus monodon

Truncated VP28 as oral vaccine candidate against WSSV infection in shrimp: An uptake and processing study in the midgut of Penaeus monodon

Survival in juvenile shrimps (Penaeus vannamei) exposed to inactive against active white spot virus: a challenge bioassay perspective

CHARACTERISTICS OF VIRAL PROTEIN, VP-15, OF WHITESPOT SYNDROME VIRUS ISOLATED FROM INFECTED TIGER SHRIMP Penaeus monodon (Fabricius, 1798)

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