Protection of Mice from Lethal Endogenous <i>Candida albicans</i> Infection by Immunization with <i>Candida</i> Membrane Antigen

Tansho, Shigeru; Ab, Shigeru; Mizutani, Shigetoshi; Ono, Yasuo; Takesako, Kazutoh; Yamaguchi, Hideyo

doi:10.1111/j.1348-0421.2002.tb02700.x

Cited by 8 publications

(4 citation statements)

References 13 publications

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“…Preparation of Candida inoculate for challenge C. albicans TIMM 0239 12,13 was grown in Sabouraud dextrose broth in an L-tube. After growing at 378C overnight, cells were harvested by centrifugation, washed three times with saline, and adjusted to a cell density appropriate for inoculation to mice.…”

Section: Methodsmentioning

confidence: 99%

“…We reported previously that application of antibiotics and anti-inflammatory corticosteroids induced an overgrowth of C. albicans in the intestinal tract of mice. 12 Here, we find that prednisolone treatment of mice intestinally colonized by C. albicans induced production of specific IgE and IgG antibodies, perhaps through systemic dissemination of this fungus from the gut.…”

Section: Introductionmentioning

confidence: 93%

“…For immunosuppression, animals were subcutaneously given 100 mg/kg body weight of prednisolone (Mitaka Seiyaku, Tokyo, Japan) 7 and 9 days after the Candida challenge as described elsewhere. 12 Intestinal colonization of C. albicans was monitored by counts of viable C. albicans cells in their stools as follows. Every stool sample was homogenized in a volume of sterile saline and serial 10-fold dilutions with saline were made.…”

Section: Animals and Inoculationmentioning

confidence: 99%

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Production of anti‐Candida antibodies in mice with gut colonization of Candida albicans

Tansho

Abe

Ishibashi

et al. 2004

Mediators of Inflammation

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BACKGROUND: Production of antibodies that are specific for allergens is an important pathological process in inflammatory allergic diseases. These contain the antibodies against antigens of Candida albicans, one of the normal microbial flora in an intestinal tract. We studied the effects of the prednisolone administration on the production of anti-Candida antibodies in the gastrointestinally C. albicans-colonized mice. METHODS AND MATERIALS: BALB/c mice, treated with antibacterial antibiotics to decontaminate indigenous intestinal bacterial flora, were inoculated intragastrically with C. albicans. The mice, in which C. albicans grows intestinally, were administered prednisolone to induce temporary immunosuppression. The Candida growth in their intestinal tract and their antibody response to Candida were examined. RESULTS: Antibiotic treatment allowed establishment of C. albicans gastrointestinal colonization, but did not cause subsequent systemic dissemination of C. albicans in all the animals. When these animals received an additional treatment with prednisolone, they showed a significantly higher population of C. albicans in their feces than those of animals treated with antibiotics alone, and the organisms were recovered even from their kidney. This systemic dissemination by C. albicans appeared to be temporal, because all the mice survived without any symptoms for more than 2 months. Examination of the serum titers of total immunoglobulin (Ig)E antibodies and specific IgE and IgG antibodies against Candida antigens demonstrated that titers of total IgE increased, partially by day 14 and clearly at day 27, in prednisolone-treated Candida-colonized mice. Without prednisolone treatment, an increment of the serum titer was scarcely observed. By day 27, corresponding to the increase of total IgE, the anti-Candida IgE and IgG titer increased in mice of the prednisolone-treated group. CONCLUSION: Administration of prednisolone to Candida-colonized mice can induce production of the IgG, IgE antibodies against Candida antigens, perhaps through temporal systemic dissemination of Candida from the intestinal tract.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

Section: Animals and Inoculationmentioning

confidence: 99%

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Production of anti‐Candida antibodies in mice with gut colonization of Candida albicans

Tansho

Abe

Ishibashi

et al. 2004

Mediators of Inflammation

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“…Each mouse in the groups was injected with 50 ul of P.aeruginosa in day 5 in the right foot pad and normal saline in the left foot pad (except control group), arthus reaction was observed and determined as an increase in footpad swelling 4 hrs while delayed type hypersensitivity peak after 24 and 48 hours by a digital vernia and given in a unit of millimeter, as suggested by (Tansho et al, 2002) [13] .…”

Section: Arthus and Delayed Hypersensitivity Testmentioning

confidence: 99%

Effect of Staphylococcus aureus Thermostable DNase on Some Immunological Parameters

Noor Naeem Khwen,

Sawsan Hassan Authman,

Mohammed Faraj AL- Marjani

2021

Indian Journal of Forensic Medicine & Toxicology

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Thermostable DNase was purified from Staphylococcus aureus isolated from local clinical source. The current study includes different immunological assays to evaluate the role of purified DNase. The phagocytic activity showed that the group of mice infected with P.aeruginosa recorded the highest values with significant differences (P <0.05) in comparison with the group that received mixed injection of 1 µg /ml of DNase and P. aeruginosa showed decrease in phagocytic activity followed by group that injected by 1 µg /ml DNase only with phagocytic activity 2%. DNase has a positive immunomodulatory effect on IL-10 and IL-4 level. The data showed that the highest value of IL-10 is in group that injected with 1 µg /ml DNase only (IV group) was 86.79±1.68. While the highest value of IL-4 was in group that injected with both DNase (1 µg /mouse) and P.aeruginosa gave the highest values (III group) was 84.02 ±1.19. In Arthus reactions(Immediate type hypersensitivity), the group that have been injected with both DNase and P. aeruginosa (group III ) gave the highest values (4.50±0.70) while in delayed type hypersensitivity the same group III showed higher values after 24 and 48 hrs (5.00±0.00) , (4.00±0.00) respectively.

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Current Awareness on Yeast

2002

Yeast

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In order to keep subscribers up‐to‐date with the latest developments in their field, this current awareness service is provided by John Wiley & Sons and contains newly‐published material on yeasts. Each bibliography is divided into 10 sections. 1 Books, Reviews & Symposia; 2 General; 3 Biochemistry; 4 Biotechnology; 5 Cell Biology; 6 Gene Expression; 7 Genetics; 8 Physiology; 9 Medical Mycology; 10 Recombinant DNA Technology. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted. (3 weeks journals ‐ search completed 26th. June 2002)

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Protection of Mice from Lethal Endogenous Candida albicans Infection by Immunization with Candida Membrane Antigen

Cited by 8 publications

References 13 publications

Production of anti‐Candida antibodies in mice with gut colonization of Candida albicans

Production of anti‐Candida antibodies in mice with gut colonization of Candida albicans

Effect of Staphylococcus aureus Thermostable DNase on Some Immunological Parameters

Current Awareness on Yeast

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